Sampling frequencyNOMEECCEBAATAL12-67-12+120 20 40 60 80 100Figure 22. Benthic invertebrates sampling frequency in the GIGs.Sampling period%120100806040200AL AT BA CE EC ME NOWhole yearVegetati<strong>on</strong> periodIce free periodWinterSpringSummerAutumnFigure 23 Benthic invertebrates sampling period in the GIGs.23. In Table 5 the sampling <strong>methods</strong> and metrics for benthic invertebrates arepresented. It shows that a wide number <strong>of</strong> sampling approaches and metrics are used.The collecti<strong>on</strong> <strong>of</strong> samples is performed by several different devices: grab, Eckmanskip, sticking cylinder, triangle bottom scraper and hand net. The mesh size varieswidely from 100µm to 670µm. When kick sampling is used time (1-3 minutes), meshsize (100-670µm) and habitat sampled (littoral in general or st<strong>on</strong>es) are different.Some countries sampled in lake littoral, other the pr<strong>of</strong>undal and some in both lakez<strong>on</strong>es (IE). Two countries make use <strong>of</strong> internati<strong>on</strong>al sampling standard <strong>methods</strong>,Est<strong>on</strong>ia and Romania. These standards are currently under revisi<strong>on</strong>.24. Every country uses a different combinati<strong>on</strong> <strong>of</strong> metrics, the following metrics areeither used al<strong>on</strong>e or in combinati<strong>on</strong>: abundance and relative abundance, diversity62
indicators, species lists, frequency <strong>of</strong> occurrence <strong>of</strong> individual taxa, number <strong>of</strong> taxa,group ratios, average score per taxa, biotic score, biotic integrity index, saprobicindex, average score per tax<strong>on</strong> and ratio <strong>of</strong> littoral to pr<strong>of</strong>undal taxa.Table 5. Benthic invertebrates sampling and metrics used in different countries withinthe GIGs..GIG/CountrySampling MethodMetricDEFRIEGBPLLTEEEEALPINEsampling with grab or sticking cylinder; meshsize for sieving 0.2 mmEckman skip + strainerlittoral samples taken by kick sampling <strong>on</strong> st<strong>on</strong>y abundance, frequency <strong>of</strong> occurrence,substrate for 2 minutes using 670µm mesh size; presence <strong>of</strong> indicator taxa, group ratios andp<strong>on</strong>d net bag with rectangular frame (260mm diversitywide, 200mm high); pr<strong>of</strong>undal samples taken byEckman Grab (5 replicates) at deepest point <strong>of</strong>lake3 minute kick sample with Freshwater biotic score, number <strong>of</strong> taxa and averageBiological Associati<strong>on</strong> p<strong>on</strong>d net mesh 100um score per tax<strong>on</strong>, log abundance estimates,indicator taxa.BALTICEkmann and Birdge sampler, samples from the dominance structuredepth <strong>of</strong> 20 - 50 m5 samples taken in 1/40 m2 area by special biotic integrity indexdevice for quantitative measurements and 1sample by triangle bottom scraper, which isdragged for 2-5 metresguidance <strong>of</strong> sampling <strong>of</strong> aquatic benthic macroinvertebrates(ISO 7828:1985),(ISOspecies compositi<strong>on</strong>, number <strong>of</strong> species8265:1988),(ISO 9391:1993),(ISO 8689-1:2000),(ISO 8689-1:2000).Borutsky or Zabolotsky bottom sampler;internati<strong>on</strong>al standards: (ISO 7828:1985). (ISO8689-1:2000)(ISO 8689-2:2000).percentages <strong>of</strong> larger tax<strong>on</strong>omic groups,ASPT, EPT indexesCENTRALNL depending <strong>on</strong> substrate macroinvertebrates are no metric used for classificati<strong>on</strong>; Zebrasampled from st<strong>on</strong>es (5 st<strong>on</strong>es), or the substrate mussels: biovolume and number <strong>of</strong>itself is sampled with a grab (Ekman samples sites0.15x0.15m), or tube (diameter ca. 0.1m) orhand net (mesh 0.5 mm).GB 1 minute kick/sweep species listBE littoral, standard netDE sampling stati<strong>on</strong>s in different depths (2-3, 5, 7, indicator taxa, abundance, group ratio:10, 15, 24 m) al<strong>on</strong>g <strong>on</strong>e transect, mesh size: 405 littoral/pr<strong>of</strong>undal faunaµm; or with grab or sticking cylinder; mesh sizefor sieving 0.2 mm and 0,5 mm; or Ekman-Birge sampler, dredgeEASTERN CONTINENTALRO Knopp method; Pantle - Buck method; SR ISO density, indicator taxa, abundance, number5667-2/98<strong>of</strong> species, saprobic indexES grabber samplers species determinati<strong>on</strong>63
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Institute for Environment and Susta
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CONTENTSBackground and purpose of t
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Background and purpose of the docum
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States and candidate countries. Inf
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classification, each of these being
- Page 11 and 12: BIOLOGICAL QUALITY ELEMENT: PHYTOPL
- Page 13 and 14: indicators, species lists, frequenc
- Page 15 and 16: 53. The identification and enumerat
- Page 17 and 18: 64. In general, this technique is t
- Page 19 and 20: RIVER BIOLOGICAL QUALITY ELEMENT: F
- Page 21 and 22: 84. The development of specific sta
- Page 23 and 24: practice guides for identification
- Page 25 and 26: 100. The information received from
- Page 27 and 28: Evaluation of the suitability of cu
- Page 29 and 30: group should update its primary fin
- Page 31 and 32: ReferencesAFNOR (Association Franç
- Page 33 and 34: EN ISO 8689-2 Water quality - Biolo
- Page 35 and 36: Lazaridou-Dimitriadou, M., C. Kouko
- Page 37 and 38: Shannon, C.E. and W. Weaver, 1949.
- Page 39: Annex I: Composition of the Geograp
- Page 43 and 44: Annex III: River biological assessm
- Page 45 and 46: Annex IV: Analysis of lake biologic
- Page 47 and 48: Number of sampling stations10080%60
- Page 49 and 50: IT 90% acetone spectrophotometricPT
- Page 51 and 52: 10. The sampling depth and volume s
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- Page 55 and 56: Sampling stations%1008060402001 2-1
- Page 57 and 58: MACROPHYTES16. The aquatic Macrophy
- Page 59 and 60: Plants sampled per GIG1008060%40Eme
- Page 61: NO qualitativ method species number
- Page 65 and 66: 26. The sampling frequency is varia
- Page 67: CEN/TC 230/WG 2/ TG 4 N28, 2 nd wor
- Page 70 and 71: programs are based only on the diat
- Page 72 and 73: 21. Some countries like France, Est
- Page 74 and 75: Table 1. European methods for monit
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- Page 90 and 91: Table 5. Common abundance classific
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- Page 94 and 95: Austria120. MuLFA: Ecological Integ
- Page 96 and 97: Sweden126. Swedish fish Index: Appe
- Page 98 and 99: ut comparisons have been made with
- Page 100 and 101: seasons for sampling are summer and
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- Page 104 and 105: Consultation open to ECOSTAT &inter
- Page 106 and 107: Table 1. List of European standards
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- Page 110 and 111: development of typologySweden Yes,
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one or other option depends on the
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114
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• An integrated holistic evaluati
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• Phytoplankton: Yes; Clorophyll
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• Macroalgae: No• Benthic inver
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• Macrophytes: No• Macroalgae:
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Mission of the JRCThe mission of th