Report on Harmonisation of freshwater biological methods

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12. Table 2 shows that sampling methods and metrics often differ between countriesat GIG level, and that apart from biomass as indicated from chlorophyllconcentrations, for a specific lake or lake type each country uses different metrics.Also, there are no countries that completely fulfill the WFD metric requirements.Table 2. Phytoplankton sampling device and metrics used in different countries within theGIGs.GIG/ Sampling methodMetricCountryALPINEATabundance, biomass, group ratios, indicator taxaITabundanceAT point samples abundance, biomass, indicator taxa, group ratiosSI single depth samples temporal and spatial distribution of different speciesDE integrated samples species composition and abundance or abundance and biomassof single speciesFR net on secchi depth or onintegrated sampling from 0to 50mabundanceATLANTICIE standard net or aliquot of relative abundance, indicator taxa and group ratios, annual cycle,GBPLLVEEPLNLDEROintegrated sampleRutner water sampler everymetre from surfacecomposited, supplementedby phytoplankton net towover lake surface.Composite tube sampling.abundance estimated by analysis of the pigment chlorophyllabundance and identification of algal speciesBALTIC15 l of water filtered by dominance structure, indicator taxa.plankton net No25 from thedepth of 1m and 5m.with Ruttner bathometer, biomass, indicator taxa, relative contribution of Cyanophyta,from 0,5 m depth other Chrysophyta, Bacillariophyta etc, saprobic index, number offrom 0.2-0.3 m depth and 5-species10mRuttner sampler or VanDorn sampler Utermöhl's(1958) technique1l and qualitative drawnsample from photic zone.plankton-net no 25.abundance, frequency of occurrence, indicator taxa, group ratios,percentages of larger taxonomic groups, community structure,chlorophyll a concentrationCENTRALdominance structure, indicator taxa.cell counting (from colonies cells are estimated), identification tospecies or to genera level, share of groups (diatoms-blue – greenotheralgae)surface sample or standard No information availablewater sampler in a depth of1 m, or 5-liter integralsampler, sampling theepilimnetic layersamples takes with bucket,Knopp method SR ISOspecies density, abundance, wet biomass, biomass onsystematical gr, probic index52
PTESFIIE5667-2/98 Romanianstandardisated method(STAS-ICIM)bottle sampler andUtermohl method -qualitative analysisMEDITERRANEANspecies abundance classes (present, frequent, abundant andbloom)abundance and species compositionNORTHERNOne composite sample from biomass, species number, relative abundance.the epilimnion, usually 0 - 2m. Sampling, preservationand analysis described indetail in Swedish EPAreports 4860 and 4861.Standard net, haul from 6mfor quantitative data andsub-surface waterrelative abundance, indicator taxa and group ratiosGB 5m integrated tube, species listNO 2m tube sampler taken atsite of max.depth. Separatesamples are mixed fromdifferent depth intervals: 0-2, 2-4, 4-6 m etc. down to2x secchi depthbiomass per species for each species presentSEdepth- and surfaceintegrated pelagial samplescollected with a plexiglasstube. Sampling,preservation, and analysesdescribed in detail inSwedish EPA reports 4860and 4861total biovolumePHYTOBENTHOS13. Most countries do not use phytobenthos in their biological monitoring (Fig. 12),The highest number of lakes monitored for phytobenthos is found in the EC GIGfollowed closely by CE and AL GIGs, while in the NO GIG countries this biologicalelement is not used at all.53
Page 1 and 2: Institute for Environment and Susta
Page 3 and 4: CONTENTSBackground and purpose of t
Page 5 and 6: Background and purpose of the docum
Page 7 and 8: States and candidate countries. Inf
Page 9 and 10: classification, each of these being
Page 11 and 12: BIOLOGICAL QUALITY ELEMENT: PHYTOPL
Page 13 and 14: indicators, species lists, frequenc
Page 15 and 16: 53. The identification and enumerat
Page 17 and 18: 64. In general, this technique is t
Page 19 and 20: RIVER BIOLOGICAL QUALITY ELEMENT: F
Page 21 and 22: 84. The development of specific sta
Page 23 and 24: practice guides for identification
Page 25 and 26: 100. The information received from
Page 27 and 28: Evaluation of the suitability of cu
Page 29 and 30: group should update its primary fin
Page 31 and 32: ReferencesAFNOR (Association Franç
Page 33 and 34: EN ISO 8689-2 Water quality - Biolo
Page 35 and 36: Lazaridou-Dimitriadou, M., C. Kouko
Page 37 and 38: Shannon, C.E. and W. Weaver, 1949.
Page 39: Annex I: Composition of the Geograp
Page 43 and 44: Annex III: River biological assessm
Page 45 and 46: Annex IV: Analysis of lake biologic
Page 47 and 48: Number of sampling stations10080%60
Page 49 and 50: IT 90% acetone spectrophotometricPT
Page 51: 10. The sampling depth and volume s
Page 55 and 56: Sampling stations%1008060402001 2-1
Page 57 and 58: MACROPHYTES16. The aquatic Macrophy
Page 59 and 60: Plants sampled per GIG1008060%40Eme
Page 61 and 62: NO qualitativ method species number
Page 63 and 64: indicators, species lists, frequenc
Page 65 and 66: 26. The sampling frequency is varia
Page 67: CEN/TC 230/WG 2/ TG 4 N28, 2 nd wor
Page 70 and 71: programs are based only on the diat
Page 72 and 73: 21. Some countries like France, Est
Page 74 and 75: Table 1. European methods for monit
Page 76 and 77: countries also covers Non-EU Member
Page 78 and 79: 49. The Danish Stream Fauna Index i
Page 80 and 81: Hungary58. Since 2002 a modificatio
Page 82 and 83: Acidification Index, based on the s
Page 84 and 85: Identification is predominantly to
Page 86 and 87: size of the net range between 250 t
Page 88 and 89: water bug genus (Aphelocheirus) and
Page 90 and 91: Table 5. Common abundance classific
Page 92 and 93: 108. Process Assessment focuses on
Page 94 and 95: Austria120. MuLFA: Ecological Integ
Page 96 and 97: Sweden126. Swedish fish Index: Appe
Page 98 and 99: ut comparisons have been made with
Page 100 and 101: seasons for sampling are summer and
Page 102 and 103:
102
Page 104 and 105:
Consultation open to ECOSTAT &inter
Page 106 and 107:
Table 1. List of European standards
Page 108 and 109:
108
Page 110 and 111:
development of typologySweden Yes,
Page 112 and 113:
one or other option depends on the
Page 114 and 115:
114
Page 116 and 117:
• An integrated holistic evaluati
Page 118 and 119:
• Phytoplankton: Yes; Clorophyll
Page 120 and 121:
• Macroalgae: No• Benthic inver
Page 122 and 123:
• Macrophytes: No• Macroalgae:
Page 124:
Mission of the JRCThe mission of th
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Report on Harmonisation of freshwater biological methods

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