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Report on Harmonisation of freshwater biological methods

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12. Table 2 shows that sampling <strong>methods</strong> and metrics <strong>of</strong>ten differ between countriesat GIG level, and that apart from biomass as indicated from chlorophyllc<strong>on</strong>centrati<strong>on</strong>s, for a specific lake or lake type each country uses different metrics.Also, there are no countries that completely fulfill the WFD metric requirements.Table 2. Phytoplankt<strong>on</strong> sampling device and metrics used in different countries within theGIGs.GIG/ Sampling methodMetricCountryALPINEATabundance, biomass, group ratios, indicator taxaITabundanceAT point samples abundance, biomass, indicator taxa, group ratiosSI single depth samples temporal and spatial distributi<strong>on</strong> <strong>of</strong> different speciesDE integrated samples species compositi<strong>on</strong> and abundance or abundance and biomass<strong>of</strong> single speciesFR net <strong>on</strong> secchi depth or <strong>on</strong>integrated sampling from 0to 50mabundanceATLANTICIE standard net or aliquot <strong>of</strong> relative abundance, indicator taxa and group ratios, annual cycle,GBPLLVEEPLNLDEROintegrated sampleRutner water sampler everymetre from surfacecomposited, supplementedby phytoplankt<strong>on</strong> net towover lake surface.Composite tube sampling.abundance estimated by analysis <strong>of</strong> the pigment chlorophyllabundance and identificati<strong>on</strong> <strong>of</strong> algal speciesBALTIC15 l <strong>of</strong> water filtered by dominance structure, indicator taxa.plankt<strong>on</strong> net No25 from thedepth <strong>of</strong> 1m and 5m.with Ruttner bathometer, biomass, indicator taxa, relative c<strong>on</strong>tributi<strong>on</strong> <strong>of</strong> Cyanophyta,from 0,5 m depth other Chrysophyta, Bacillariophyta etc, saprobic index, number <strong>of</strong>from 0.2-0.3 m depth and 5-species10mRuttner sampler or VanDorn sampler Utermöhl's(1958) technique1l and qualitative drawnsample from photic z<strong>on</strong>e.plankt<strong>on</strong>-net no 25.abundance, frequency <strong>of</strong> occurrence, indicator taxa, group ratios,percentages <strong>of</strong> larger tax<strong>on</strong>omic groups, community structure,chlorophyll a c<strong>on</strong>centrati<strong>on</strong>CENTRALdominance structure, indicator taxa.cell counting (from col<strong>on</strong>ies cells are estimated), identificati<strong>on</strong> tospecies or to genera level, share <strong>of</strong> groups (diatoms-blue – greenotheralgae)surface sample or standard No informati<strong>on</strong> availablewater sampler in a depth <strong>of</strong>1 m, or 5-liter integralsampler, sampling theepilimnetic layersamples takes with bucket,Knopp method SR ISOspecies density, abundance, wet biomass, biomass <strong>on</strong>systematical gr, probic index52

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