Report on Harmonisation of freshwater biological methods

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A. International Organisation for Standardization (ISO) and ComitéEuropéen de Normalisation (CEN): General background80. The standardization of biological methods is undertaken in ISO TechnicalCommittee (TC) 147 subcommittee (SC) 5 – Biological methods and CEN/TC 230working group (WG) 2 – Biological and Ecological Assessment Methods. Thesecommittees and their parent bodies work closely together to ensure that there is nooverlap in their respective work programmes.81. The work programme of ISO/TC 147/SC 5 is primarily to develop toxicity andbiodegradability methods including the supporting statistical methodologies andhence are not relevant to the intercalibration exercise and will not be considered indetail here.82. The development of biological and ecological assessment methods is now solelywithin CEN/TC 230/WG 2, which is comprised of 6 task groups (TGs) and an ad hocstrategy group (Fig. 3).Figure 3. Structure of WG 2 biological and ecological assessment methods within the contextof the technical committee, CEN/TC 230.83. The work programme of WG 2 is in support of all legislation where there is aninherent or specific requirement under existing EU legislation for biological andecological assessment methods. However, it is obvious from the titles of the Taskgroups that WFD has a large influence on the standards being developed.20
84. The development of specific standards is very much the domain of the individualtask groups. WG 2 manages the work programme of its task groups advised by its adhoc strategy group in liaisons with expert groups, DG Environment, Joint ResearchCentre, ECOSTAT (Ecological Status) etc, in prioritising future work items for thetask groups.B. Current work programme85. The CEN/TC 230/WG 2 work programme will change over time as standards arecompleted and as new work items are initiated. Table 2 provides a summary of activeformal work items up to July 2005.Table 2. CEN/TC 230/WG 2 formal work programme (July 2005).CEN Reference Work item CommentCEN230217 Water Quality – Guidance standard for the surveying of NWIP approvedprENXXX macrophytes in lakesCEN 230175 Water Quality – Guidance standard on the routinesampling of benthic algae in fast flowing, shallowwaters to include laboratory proceduresOriginal WI deleted butNWIP will be requested toinclude expanded scopeCEN 230171 Water Quality – Guidance on the scope and selection ofPrEN14962CEN 230169prEN/ISO16665CEN 230216prEN/ISO 19493CEN 230207prEN15204CEN230209prEN 14996CEN 230208prEN15110CEN230171prEN14962CEN 230213prEN/ISO15196WI 230165CEN230172prEN 14757WIXX N72 ECEN230118prEN 14614prEN 14393prEN 8692fish sampling methods.Water Quality – Guidelines for quantitativeinvestigations of marine soft-bottom benthic fauna inthe marine environmentWater Quality – Guidance on marine biological surveysof littoral and sublittoral hard bottomWater Quality – Guidance standard for routine analysisof phytoplankton abundance and composition usinginverted microscopy (Utermöhl technique)Water Quality – Guidance on assuring the quality ofbiological and ecological assessments in the aquaticenvironmentWater Quality – Guidance standard for the routinesampling of zooplankton from standing watersWater Quality – Guidance on the scope and selection offish sampling methodsWater Quality – Guidance on the sampling andprocessing of the pupal exuviae of Chironomidae (OrderDiptera) for ecological assessmentWater quality – Guidance on data collation,interpretation and classification of running waters basedon aquatic macrophytesWater quality – Sampling of fish with multi-meshgillnetsWater quality – Guidance standard for surveying ofbenthic macro-invertebrates in lentic watersWater quality – Guidance standard for assessing thehydromorphological features of riversWater quality – Guidance on quality assurance aspectsof aquatic macrophytes surveying and analysis inrunning watersWater quality – Fresh water algal growth inhibition testISO lead but proposed forparallel adoptionCEN leadInclude in workprogramme in June 200421
Page 1 and 2: Institute for Environment and Susta
Page 3 and 4: CONTENTSBackground and purpose of t
Page 5 and 6: Background and purpose of the docum
Page 7 and 8: States and candidate countries. Inf
Page 9 and 10: classification, each of these being
Page 11 and 12: BIOLOGICAL QUALITY ELEMENT: PHYTOPL
Page 13 and 14: indicators, species lists, frequenc
Page 15 and 16: 53. The identification and enumerat
Page 17 and 18: 64. In general, this technique is t
Page 19: RIVER BIOLOGICAL QUALITY ELEMENT: F
Page 23 and 24: practice guides for identification
Page 25 and 26: 100. The information received from
Page 27 and 28: Evaluation of the suitability of cu
Page 29 and 30: group should update its primary fin
Page 31 and 32: ReferencesAFNOR (Association Franç
Page 33 and 34: EN ISO 8689-2 Water quality - Biolo
Page 35 and 36: Lazaridou-Dimitriadou, M., C. Kouko
Page 37 and 38: Shannon, C.E. and W. Weaver, 1949.
Page 39: Annex I: Composition of the Geograp
Page 43 and 44: Annex III: River biological assessm
Page 45 and 46: Annex IV: Analysis of lake biologic
Page 47 and 48: Number of sampling stations10080%60
Page 49 and 50: IT 90% acetone spectrophotometricPT
Page 51 and 52: 10. The sampling depth and volume s
Page 53 and 54: PTESFIIE5667-2/98 Romanianstandardi
Page 55 and 56: Sampling stations%1008060402001 2-1
Page 57 and 58: MACROPHYTES16. The aquatic Macrophy
Page 59 and 60: Plants sampled per GIG1008060%40Eme
Page 61 and 62: NO qualitativ method species number
Page 63 and 64: indicators, species lists, frequenc
Page 65 and 66: 26. The sampling frequency is varia
Page 67: CEN/TC 230/WG 2/ TG 4 N28, 2 nd wor
Page 70 and 71:
programs are based only on the diat
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21. Some countries like France, Est
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Table 1. European methods for monit
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countries also covers Non-EU Member
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49. The Danish Stream Fauna Index i
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Hungary58. Since 2002 a modificatio
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Acidification Index, based on the s
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Identification is predominantly to
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size of the net range between 250 t
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water bug genus (Aphelocheirus) and
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Table 5. Common abundance classific
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108. Process Assessment focuses on
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Austria120. MuLFA: Ecological Integ
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Sweden126. Swedish fish Index: Appe
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ut comparisons have been made with
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seasons for sampling are summer and
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102
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Consultation open to ECOSTAT &inter
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Table 1. List of European standards
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108
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development of typologySweden Yes,
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one or other option depends on the
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114
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• An integrated holistic evaluati
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• Phytoplankton: Yes; Clorophyll
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• Macroalgae: No• Benthic inver
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• Macrophytes: No• Macroalgae:
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Mission of the JRCThe mission of th
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Report on Harmonisation of freshwater biological methods

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