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Report on Harmonisation of freshwater biological methods

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53. The identificati<strong>on</strong> and enumerati<strong>on</strong> <strong>of</strong> relative proporti<strong>on</strong>s <strong>of</strong> diatom taxa is based<strong>on</strong> a European Standard EN 14407 (2004) Water quality: Guidance standard for theidentificati<strong>on</strong>, enumerati<strong>on</strong> and interpretati<strong>on</strong> <strong>of</strong> benthic diatom samples fromrunning water. Some countries use the OMNIDIA s<strong>of</strong>tware for tax<strong>on</strong>omicidentificati<strong>on</strong> (Lecointe et al., 1993), which allows the calculati<strong>on</strong> <strong>of</strong> a great number<strong>of</strong> diatom indices and c<strong>on</strong>tains a systematic and an ecological database. C<strong>on</strong>sultati<strong>on</strong><strong>of</strong> other literature is also recommended for the algal identificati<strong>on</strong>, like floras,identificati<strong>on</strong> guides and ic<strong>on</strong>ographs appropriated to the habitats and geographicregi<strong>on</strong> under c<strong>on</strong>siderati<strong>on</strong>.54. The recording <strong>of</strong> abundance is usually expressed as the number <strong>of</strong> cells per tax<strong>on</strong>per sample <strong>on</strong> occasi<strong>on</strong>s using abundance classes. Relative coverage <strong>of</strong> the river bedis also menti<strong>on</strong>ed by <strong>on</strong>e country.55. Three different categories <strong>of</strong> assessment <strong>methods</strong> were identified in this review:biotic indices, assemblage/community assessment and multimetric indices.Bioticindices are the most comm<strong>on</strong> assessment method including 5 different approaches allbased in diatom assemblages, applied to 9 countries. Only <strong>on</strong>e country is usingassemblage/community assessment (Austria) and two are using multimetric indicesEst<strong>on</strong>ia and Germany). The Biological Diatom Index’ (IBD- Indice BiologiqueDiatomées; AFNOR, 2000) is the <strong>on</strong>ly standardized method applied <strong>on</strong> a nati<strong>on</strong>allevel.RIVER BIOLOGICAL QUALITY ELEMENT: MACROPHYTES56. Macrophytes are m<strong>on</strong>itored in ten countries <strong>of</strong> the European Uni<strong>on</strong> also includingNorway. All <strong>methods</strong> comprise the investigati<strong>on</strong> <strong>of</strong> watercourse vegetati<strong>on</strong> by means<strong>of</strong> regular visual from the banks or by wading in the stream to record the tax<strong>on</strong>omiccompositi<strong>on</strong> and abundance <strong>of</strong> water plants. Assessment <strong>of</strong> the ecological quality <strong>of</strong>watercourses is d<strong>on</strong>e by five different schemes. Recently 3 countries have developedmacrophyte <strong>methods</strong> to meet the requirements <strong>of</strong> the WFD.57. Sampling area differs between individual <strong>methods</strong>: separate transects <strong>of</strong> 0.25 x0.25 m² are sampled, survey an area <strong>of</strong> at least 100 m², other schemes prescribe theinvestigati<strong>on</strong> <strong>of</strong> stream reaches <strong>of</strong> 100 m or 500 m. Only two countries follow thestandard EN 14184:2003 Water quality: Guidance standard for the surveying <strong>of</strong>aquatic macrophytes in running waters.15

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