ILP F F (Jatropha curcas L.) Development of ILP Marker to Assess ...

20 1 ก - 2555 ก กก (Tanngue, D2, Rwar-Ngn) กกก กกก( 2)3.4 กกกกกก 6 ก ก ( 1) กกกก ก 84.51% () 90.14%() 96.71% Neis geneticdiversity (He) Shannon index (I) 0.39-0.46 0.51-0.61 0.43 0.60 ( 4) กกก กก genetic diversity ก กก กก ก ก ne(effective number of allele) กก กก (4-17ก) กกกกกกก Gst (coefficient of genedifferentiation) ก 0.06 กกกกกกกก ก Nm75(gene flow index) ก (3.64) กกกก 4 กกกก 6 ก ก ILPGroup n na ne He INorth 15 2.11 1.84 0.43 0.61Northeast 6 1.97 1.85 0.46 0.61Central 17 1.95 1.82 0.42 0.59South 4 1.90 1.80 0.46 0.58Asia 11 2.18 1.77 0.40 0.59USA and Africa 6 1.88 1.70 0.39 0.51Total 59 2.47 1.84 0.43 0.60SD 0.12 0.06 0.03 0.04Ht Hs Gst Nm0.42 0.42 0.06 3.64Na = Number of allelesne = Number of effective allelesHe = Neis genetic diversityI = Shannon indexHt = Total gene diversityNm = gene flow indexGst = coefficient of gene differentiation3.5 กกกก J. curcasกก 59 6 ก Fst กก( 5) Fst กกก (Fst = -0.04052) กกกก (Fst =

20 1 ก - 2555-0.023) (p>0.05) Fst กกกกกกกก กก gene flow ก กกกก Fstกกกกกกก 0.1406 กก geneflow ก กกกกกก กก genetic identity UPGMA dendrogram 3 กก กกกกกกกก coefficient of genetic differentiationกก (G ST ) ก 0.06 กก AMOVA กกก 96.86% ( 6) กกกกกกกก 3 Dendrogram กกกก 6 ก4. ก4.1 ILP ILP กกก microsatellite ก กกกกกกกก [19] 5 ก () Fst () ILPGroups North Northeast Central South Asia USA/AfricaNorth - 0.9891 0.9946 0.9591 0.9511 0.9095Northeast -0.0405 ns - 0.9855 0.9550 0.9423 0.9040Central -0.0230 ns -0.0309 - 0.9565 0.9393 0.8962South -0.0113 -0.0376 -0.0043 - 0.9325 0.9000Asia 0.0351 0.0242 0.0534 0.0254 - 0.9597USA/Africa 0.1218 0.1044 0.1406 0.1128 0.0425 -ns not significant at p

20 1 ก - 2555 2 Dendrogram กก77

20 1 ก - 2555 6 Analysis of molecular variance (AMOVA) กก 6 กSource ofSum of Variance Percentage ofd.f.Variationsquares Component variationF STAmong groupWithin group5112128.051785.470.51716Va15.9417Vb3.1496.860.03142 ก กกก ก กก [20,21] ILP codominant กก กกก กก กกกกกกก ILPกก กก EST ก Euphorbiaceae กก ก ILP 84% กILP กกกก ILPกกกกก [3,4]กกกกกกกกก Ming et al. (2010) [22] กกก ILP SSR ILP กกกกกก 4.2 กกก ก กกกกกกก กกกกกก กก ก ก RAPD ISSR, AFLP, gSSR EST-SSR กกก [7,23,24,25,26] กกกก ILP กก78

20 1 ก - 2555 ILP กกกกกกกก กกก (Ht=0.44) กก กกกกกกกก กกกกก กกกกกกก [27] กกกก กกก ก AMOVA กก กกก ก กก กกกกกกกก ก(germplasm) กก กกกกก กกก กก F ST ( 5) กกกกกกกกกกกกกกก กกก 79ก กก กกกกกก dendrogram กกกก6 ก ( 2) กกกกกก กกกกกก ก F ST กกกก กกกกก ก F ST กก- (0.39) กก (0.51) กก ( 4) กกกกกกกกกกกกก Namlat กกกกก NE-CP กก5. ก ILP 71 กกกก

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