République Tunisienne - Ecole Nationale d'ingénieurs de Sfax

- Explorer les systèmes digestifs des animaux marins pour pouvoir dégager des traits communs entreles systèmes lipolytiques des différentes classes d’animaux marins (poissons osseux, poissonscartilagineux, arthropodes…).- Isoler les gênes codant pour les lipases de scorpion, de raie et de l’anguille pour déterminer lesséquences complètes en acides aminés de ces enzymes digestives et établir les relationsphylogénétiques avec les autres familles de lipases.- Explorer les possibilités d’utilisation un de ces enzymes lipolytiques d’animaux inférieurs commeagents thérapeutiques potentiels.- Purifier et caractériser des phospholipases de différentes origines (hépatopancréas, pancréas, venins,microorganismes).- Relations structure-fonctions de phospholipases anti-inflammatoires.- Relation entre activité phospholipasique et maladies.- Recherche d’inhibiteurs de phospholipases anti-inflammatoires.Dernières publications (2007-2009)20071. Habib Mosbah, Adel Sayari, Robert Verger and Youssef Gargouri. Gly311 residue triggers the enantioselectivity ofStaphylococcus xylosus lipase: A monolayer study. J Colloid Interface Sci. (2007) 310:196-204.2. Nacim Zouari, Nabil Miled, Souad Rouis and Youssef Gargouri. Scorpion digestive lipase: a member of a newinvertebrate's lipase group presenting novel characteristics. Biochimie. (2007) 89:403-409.3. Slim Cherif, Ahmed Fendri, Nabil Miled, Heykel Trabelsi, Hafedh Mejdoub and Youssef Gargouri. Crab digestivelipase acting at high temperature: Purification and biochemical characterization. Biochimie. (2007) 89: 1012-1018.4. Hanen Ghamgui, Nabil Miled, Maha Karra-chaâbouni and Youssef Gargouri. Immobilization studies andbiochemical properties of free and immobilized Rhizopus oryzae lipase onto CaCO3: A Comparative study. BiochemicalEngineering Journal (2007) 37:34-41.5. Adel Sayari, Habib Mosbah, Robert Verger and Youssef Gargouri. The N-terminal His-tag affects theenantioselectivity of Staphylococcal lipases: A monolayer study. Journal of Colloid and interface Science. (2007) 313:261-267.6. Habib Mosbah, Adel Sayari, Habib Horchani and Youssef Gargouri. Involvemnt of Gly311 residue on substratediscrimination, pH and temperature dependency of recombinant Staphylococcus xylosus lipase: A study with emulsifiedsubstrate. Protein Expression and purification. (2007) 55: 31-39.2Created with Print2PDF. To remove this line, buy a license at: http://www.software602.com/

7. Abir Ben Bacha, Ahmed Fendri, Youssef Gargouri, Hafedh Mejdoub and Nabil Miled. Proteolytic Cleavage of twobird pancreatic lipases: production of an active N-terminal domain. Pancreas. (2007). 35: 55-61.8. Adel Sayari et Youssef Gargouri. Synthèse enzymatique de triaglycérols homogènes dans un milieu sans solvent.Arch.Inst.Pasteur ; Tunis (2006) 83 : 1-4.Publié en 2007.9. Ahmed Fendri, Fakher Frikha, Nabil Miled, Abir Ben Bacha and Youssef Gargouri. Modulating the activity ofavian pancreatic lipases by alkyl chain reacting with an accessible sulfhdryl group. BBRC. (2007) 360: 765-771.10. Abir Ben Bacha, Youssef Gargouri, Sofiane Bezzine, Habib Mosbah and Hafedh Mejdoub.Ostrich pancreacticphospholipase A2: Purification and biochemical characterization. Journal of Chromatography B (2007)11. Riadh Ben Salah, Hanen Ghamgui, Nabil Miled, Hafedh Mejdoub and Youssef Gargouri. Production of butylacetate ester by lipase from novel strain of Rhizopus oryzae. Journal of Bioscience and Bioengineering. (2007) 103: 368-372.12. Imen Aissa, Mohamed Bouaziz, Hanen Ghamgui, Amel Kammoun, Nabil Miled, Sami Sayadi and YoussefGargouri. Optimization of Lipase-Catalysed synthesis of acetylated Tyrosol by response surface methodology J. Agric.Food Chem. (2007) 55: 10298-10305.200813. Slim Cherif, Fakher Frikha, Youssef Gargouri and Nabil Miled. Fatty acid composition of green crab (Carcinusmediterraneus) from the Tunisian Mediterranean coasts. Food Chemistry (2008) 111:930-93314. Maher Kammoun, Youssef Gargouri and Sofiane Bezzine. Purification and Characterization of the First ReptilePancreactic Lipase. Pancreas (2008) 36: 302-30815. Fakher Frikha, Moncef Ladjimi, Youssef Gargouri and Nabil Miled. 3-D structure modelling and biochemicalcharacterization of the Staphylococcus simulans lipase: conformational changes, substrate specificity and novel structuralfeatures. FEMS Microbiol Lett (2008) 286: 207-22116. Maha Karra-Chaabouni, Ines Bouaziz, Sami Boufi, Ana Maria Botelho do Rego and Youssef Gargouri. Physicalimmobilization of Rhizopus oryzae lipase onto cellulose substrate: Activity and stability. Colloids and Surface B:Biointerfaces (2008) 66:168-17717. Josiane De Caro, Cecilia Eydoux, Slim Cherif, Regine Lebrun, Youssef Gargouri, Frederic Carrière and Alain DeCaro. Occurrence of pancreatic lipase-related protein-2 in various species and its relationship with herbivore diet.Comparative Biochemistry and Physiology B (2008) 150 (1): 1-918. Faouzi Ben Rabah, Fakher Frikha, Walid Kamoun, Lobna Belbahri, Youssef Gargouri and Nabil Miled. Cultureof Staphylococcus xylosus in fish processing by –product-based media for lipase production. Letters in AppliedMicrobiology (2008) ISSN 0266-82543Created with Print2PDF. To remove this line, buy a license at: http://www.software602.com/

19. Faouzi Ben Rabah, Sana Smaoui, Fakher Frikha, Youssef Gargouri and Nabil Miled. Inhibotory Effects of TunisianMarine Algal Extracts on Digestive Lipases. Appl Biochem Biotechnol (2008) doi: 10.1007200920. Slim Cherif and Youssef Gargouri. Thermoactivity and effects of organic solvents on digestive lipase fromhepatopancreas of the green crab. Food Chemistry. (2009), 116: 82-8621. Dhouha Ghribi, Adel Sayari, Youssef Gargouri and Sofiane Bezzine. Improvement of Staphylococcus xylosus lipaseproduction through optimization of the culture conditions. Eur. J. Lipid ScI. Technol (2009) doi: 10.1002/ ejlt. 20090000422. Ikram Jemel, Ahmed Fendri, Youssef Gargouri, and Sofiane Bezzine. Kinetic properties of dromedary pancreaticlipase: A comparative study on emulsified and monomolecular substrate. Colloids and Surfaces B: Biointerfaces (2009)70 : 238-24223. Habib Horchani, Habib Mosbah, Nadia Ben Salem, Youssef Gargouri and Adel Sayari. Biochemical and molecularcharacterisation of a thermoactive, alkaline and detergent-stable lipase from a newly isolated Staphylococcus aureusstrain. Journal of Molecular Catalysis B: Enzymatic (2009) 56: 237-24524. Aida Karray, Fakher Frikha, Abir Ben Bacha, Yassine Ben Ali and Youssef Gargouri. Biochemical and molecularcharacterization of purified chicken pancreatic phospholipase A 2 .FEBS Journal (2009) 276: 4545-455425. Slim Cherif, Abir Ben Bacha, Yassine Ben Ali, Habib Horchani, Wiem Rekik, Youssef Gargouri. Crab digestivephospholipase: A new invertebrate member. Bioresource Technology, Volume 101, Issue 1, January 2010, Pages 366-371.26. Raoudha Zouari-Kessentini, José Luis, Aida Karray, Olfa Kallech-Ziri, Najet Srairi-Abid, Amine Bazaa, ErwannLoret, Sofiane Bezzine, Mohamed El Ayeb, Naziha Marrakchi. Two purified and characterized phospholipases A2from Cerastes cerastes venom that inhibits cancerous cell adhesion and migration. Toxicon, (2009) 53: 444-453.27. Slim Cherif, Abir Ben Bacha, Yassine Ben Ali, Habib Horchani, Wiem Rekik, Youssef Gargouri. Crab digestivephospholipase: A new invertebrate member (2009) doi:10.1016/j.biortech.2009.07.03128. Habib Horchani, Selmene Ouertani, Youssef Gargouri, Adel Sayari .The N- terminal His-tag and the recombinationprocess affect the biochemical properties of lipase produced in Echerichia coli. Journal of Molecular Catalysis B:Enzymatic. doi:10.1016/j.molcatb.(2009).07.00229. Habib Horchani, Moncef Chaâbouni, Youssef Gargouri, Adel Sayari. Solvent-free lipase-catalyzed synthesis of longchainstarch esters using micro-wave heating. Optimization by response surface methodology. Carbohydrate Polymers10.1016/j.carbpol.2009.09.0034Created with Print2PDF. To remove this line, buy a license at: http://www.software602.com/

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