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in advanced TripleQ instruments• a list a putatively interesting proteins is generated based onactual biological knowledge (multiplexing) or hypothesis• proteotypic peptides are selected from experience or predicted,their <strong>MS</strong>/<strong>MS</strong> spectra are known and a few <strong>MS</strong>/<strong>MS</strong>transitions selected• the <strong>MS</strong> system carries out targeted <strong>MS</strong>/<strong>MS</strong> analyses of onlythese few specific <strong>MS</strong>/<strong>MS</strong> transitions( highest possible sensitivity)

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