(MS)?
(MS)? (MS)?
(self)critical discussion about proteomics strategiesNATURE|VOL 429 | 3 JUNE 2004
the actual Proteomics debatediscovery high-throughputapproach finally deliveringon its promise"Proteomics has progressed radically in the last 5 years and is now on par with most genomictechnologies in throughput and comprehensiveness. Analyzing peptide mixtures by liquidchromatography coupled to high-resolution mass spectrometry (LC-MS) has emerged as the maintechnology for in-depth proteome analysis .... MS-based proteomics is rapidly becoming quantitativethrough both label-free and stable isotope labeling technologies. The latest generation of massspectrometers combines extremely high resolving power,mass accuracy, and very high sequencingspeed in routine proteomic applications...Thus, in a few specialized laboratories, ‘‘precision proteomics’’ can nowidentify and quantify almost all fragmented peptide peaks.
- Page 1 and 2: From Small-Molecule Drugs to Proteo
- Page 5 and 6: Relative AbundanceTandemMSorMS/MSM
- Page 8 and 9: 5000400030002000conferenceuntil 200
- Page 10 and 11: MALDI- Matrix AssistedLaser Desorpt
- Page 12: nevertheless a difficult start
- Page 16: can an exact protein molecular mass
- Page 20 and 21: % Intensity% IntensityNative MALDI
- Page 22 and 23: ESI and today also MALDI solved an
- Page 24 and 25: Small-molecule MALDIQuantification
- Page 26: Analyte/IS*ConcAnalyte/IS*Conc10.90
- Page 32 and 33: in order to analyze a proteome key
- Page 34: 1-the conventional way :"MALDI pept
- Page 37: Protein Identifizierung : ESI or MA
- Page 40 and 41: in order to analyze a proteome key
- Page 44 and 45: Remaining problems :• possibly lo
- Page 48 and 49: ut with large efforts and investmen
- Page 51: the alternative.....back to hypothe
- Page 55 and 56: A new tool MALDI imaging mass spect
- Page 57: AK KarasU. Bahr, Ph.DB. Meyer, Ph.D
(self)critical discussion about proteomics strategiesNATURE|VOL 429 | 3 JUNE 2004
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