(MS)?
(MS)? (MS)?
% Intensity% IntensityNative MALDI mass spec for siRNANative10090807060StandardSS 2DS12071.0 12211.8 12352.6 12493.4 12634.21.3E+4MALDI-MS andMS/MS for smallRNA delivers :50403020106263.86129.66020 6086 6152 6218 6284 635012380.803600 5580 7560 9540 11520 13500Mass (m/z)• stability of RNAdouble strands• excess of singlestrands1009080706050StandardDenatured1.2E+4when combinedto acid hydrolysis• RNA sequence(also for modified)40302010unspecific dimers03600 5580 7560 9540 11520 13500Mass (m/z)
ESI and today also MALDI solved an old analytical problem routine, sensitive and robust coupling of HPLC to MSfused silica capillaryID 50-75 µm
- Page 1 and 2: From Small-Molecule Drugs to Proteo
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- Page 8 and 9: 5000400030002000conferenceuntil 200
- Page 10 and 11: MALDI- Matrix AssistedLaser Desorpt
- Page 12: nevertheless a difficult start
- Page 16: can an exact protein molecular mass
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- Page 24 and 25: Small-molecule MALDIQuantification
- Page 26: Analyte/IS*ConcAnalyte/IS*Conc10.90
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- Page 34: 1-the conventional way :"MALDI pept
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- Page 40 and 41: in order to analyze a proteome key
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- Page 46 and 47: (self)critical discussion about pro
- Page 48 and 49: ut with large efforts and investmen
- Page 51: the alternative.....back to hypothe
- Page 55 and 56: A new tool MALDI imaging mass spect
- Page 57: AK KarasU. Bahr, Ph.DB. Meyer, Ph.D
% Intensity% IntensityNative MALDI mass spec for siRNANative10090807060StandardSS 2DS12071.0 12211.8 12352.6 12493.4 12634.21.3E+4MALDI-<strong>MS</strong> and<strong>MS</strong>/<strong>MS</strong> for smallRNA delivers :50403020106263.86129.66020 6086 6152 6218 6284 635012380.803600 5580 7560 9540 11520 13500Mass (m/z)• stability of RNAdouble strands• excess of singlestrands1009080706050StandardDenatured1.2E+4when combinedto acid hydrolysis• RNA sequence(also for modified)40302010unspecific dimers03600 5580 7560 9540 11520 13500Mass (m/z)
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