chitosan and plga microspheres as drug delivery ... - UniCA Eprints
chitosan and plga microspheres as drug delivery ... - UniCA Eprints
chitosan and plga microspheres as drug delivery ... - UniCA Eprints
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6. RFP Loaded PLGA Microspheres Prepared by Solvent Evaporation Method30% Mucin Adsorbed25201510500,025 mg/ml mucin 0,1 mg/ml mucin 0,5 mg/ml mucinFigure 6.7: Mucoadhesive Properties of PLGA Microspheres6.2.8. Viability Studies with A549 CellsThe cytotoxic effects of RFP against A549 cells were examined by MTT <strong>as</strong>say. In theexperiments, the cytotoxicity w<strong>as</strong> evaluated by varying the concentration of RFP <strong>and</strong> RFPentrapped in PLGA <strong>microspheres</strong>. Cytotoxicity w<strong>as</strong> observed to be concentration-dependentfor free RFP <strong>and</strong> for the formulations. Higher cytotoxic effects were found for free RFP. Infact, <strong>as</strong> can be seen in figure 6.8, also with the lowest concentration of RFP the viability isvery low. This means that the free <strong>drug</strong> can cause side effects when it is nebulized directlyinto the lung, where the infection is. Even using the smallest concentration, the free RFP isable to kill the lung cells (epithelial cells similar to A549 cells). As written before, the targetof this work w<strong>as</strong> especially the macrophages where mycobacteria are able to survive <strong>and</strong>replicate. As can be seen from the figure, encapsulation of RFP in the PLGA <strong>microspheres</strong> ledto a decre<strong>as</strong>e of the cytotoxicity, which w<strong>as</strong> quite close to that of the free <strong>drug</strong> only at thelowest tested RFP concentration .98
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