chitosan and plga microspheres as drug delivery ... - UniCA Eprints

Table Of Contents1. GENERAL INTRODUCTION 91.1. Pulmonary Mycobacterial Infections and Immuno-Compromised Hosts 101.2. Alveolar Macrophages and MTB/MAC 141.3. Lung Anatomy 161.4. Pulmonary Drug Delivery Following Aerosol Therapy 181.4.1. Delivery Device: Jet Nebulizer 181.4.2. Mechanism of Drug Deposition 201.4.3. Factors Controlling Respiratory Drug Deposition 211.4.4. Respiratory Tract Anatomy 221.4.5. Respiratory Patterns 231.4.6. Pulmonary Clearance 231.4.7. Mucociliary Clearance 241.5. Rifampicin 241.6. Microspheres as Controlled Delivery Systems 261.7. Bioadhesive Microspheres as Systems Able to Enhance Pulmonary Drug Delivery 291.8. Microspheres for Inhalation 302. CHITOSAN AND PLGA: GENERAL INFORMATIONS 352.1. Chitosan and Chitosan Microspheres as Controlled Delivery System 362.2. PLGA and PLGA Microspheres as Controlled Delivery System 403. AIM OF THE WORK 494. RIFAMPICIN LOADED CHITOSAN MICROSPHERES PREPARED BYPRECIPITATION METHOD 534.1. Materials and Methods 544.1.1. Material 544.1.2. Preparation of RFP-Loaded Chitosan Microspheres 544.1.3. Characterization of RFP-Loaded Chitosan Microspheres 554.1.4. Release/Stability Studies 574.1.5. Mucoadhesive Studies 574.1.6. Cell Culture 584.1.7. Statistical Analyses 594.2. Result and Discussion 594.2.1. Preparation of RFP-Loaded Chitosan Microspheres 594.2.2. Size and Morphological Characteristics of Microspheres 604.2.3. Surface Charge 614.2.4. Entrapment Efficiency (E%) 614.2.5. Nebulization Studies of Chitosan Microspheres 624.2.6. Release/Stability Studies 654.2.7. Mucoadhesive Studies 675