â Realizing the potential of IonWorks screening in a drug discovery ...

More documents

Recommendations

Info

Cell passage and growth phase are an integralpart of assay designNa v Cell Confluence (%) vs Time (hrs)Passage 7150K cells/ml50K cells/mlPassage 17Seeding densities influence cell growth ratesGrowth rates change over time withincreasing cell passage3 hours – 10% Confluence 48 hours – 40% Confluence 72 hours – 70% Confluence
Growth kinetics dictate experimental timingThe optimal assay window is defined by desired growth kinetics andexperimental timing
Page 1 and 2: Realizing the potential of IonWorks
Page 3 and 4: Groton ion channel drug discoveryCN
Page 5 and 6: Electrophysiology has an integralro
Page 7: Assessment of cell growth parameter
Page 11 and 12: Variables influencing assay success
Page 13 and 14: The importance of voltage control o
Page 15 and 16: Sample ion channel recordingPre-com
Page 17 and 18: Assay validation post-relocation•
Page 19 and 20: Resolution approachRe-chlorideE-hea
Page 21 and 22: I-V and inactivation curvesre-visit
Page 23 and 24: Pre- to post-addition current ampli
Page 25 and 26: Summary• Cell line husbandry and
Page 27 and 28: Back-up slides
Page 29 and 30: Kv70Single hole mode70Population pa

â Realizing the potential of IonWorks screening in a drug discovery ...

You also want an ePaper? Increase the reach of your titles

Delete template?

Save as template?

â Realizing the potential of IonWorks screening in a drug discovery ...