â Realizing the potential of IonWorks screening in a drug discovery ...

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IonWorks application in drug discoverycase study•Na v Assay– Cell husbandry and preparation– Assay format– Indices of efficacy– Targeting pathophysiological states– Facilitate lead compound selection
Assessment of cell growth parameters areparamount to maximize screening success• IncuCyte – networked, incubator-based cell imaginganalysis system– Eliminates subjectivity– Characterizes growth kinetics– Characterizes most appropriate seeding densities– Evaluate expression as a function of confluence– Key for consistency and experimental scheduling
Page 1 and 2: Realizing the potential of IonWorks
Page 3 and 4: Groton ion channel drug discoveryCN
Page 5: Electrophysiology has an integralro
Page 9 and 10: Growth kinetics dictate experimenta
Page 11 and 12: Variables influencing assay success
Page 13 and 14: The importance of voltage control o
Page 15 and 16: Sample ion channel recordingPre-com
Page 17 and 18: Assay validation post-relocation•
Page 19 and 20: Resolution approachRe-chlorideE-hea
Page 21 and 22: I-V and inactivation curvesre-visit
Page 23 and 24: Pre- to post-addition current ampli
Page 25 and 26: Summary• Cell line husbandry and
Page 27 and 28: Back-up slides
Page 29 and 30: Kv70Single hole mode70Population pa

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