â Realizing the potential of IonWorks screening in a drug discovery ...

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IonWorks application in drug discovery casestudy #2Kv assay format• HT & PPC modes• 50:50Trypsin/Versene mixto lift cells• Amphotericin B togain access• Pulse to -20mV from-80mV for 3 sec.
Kv70Single hole mode70Population patch mode606030μM retagabinecontrol5050Cell count40304030controlactivation10μM XE991inhibition20200.2nA0.2nA10102s2s000.0 0.2 0.4 0.6 0.8 1.0 1.2 0.0 0.2 0.4 0.6 0.8 1.0 1.2Kv current (nA)Population patch mode assay increases datafidelity and throughput 4xIonWorks assay can identify activators and inhibitors
Page 1 and 2: Realizing the potential of IonWorks
Page 3 and 4: Groton ion channel drug discoveryCN
Page 5 and 6: Electrophysiology has an integralro
Page 7 and 8: Assessment of cell growth parameter
Page 9 and 10: Growth kinetics dictate experimenta
Page 11 and 12: Variables influencing assay success
Page 13 and 14: The importance of voltage control o
Page 15 and 16: Sample ion channel recordingPre-com
Page 17 and 18: Assay validation post-relocation•
Page 19 and 20: Resolution approachRe-chlorideE-hea
Page 21 and 22: I-V and inactivation curvesre-visit
Page 23 and 24: Pre- to post-addition current ampli
Page 25 and 26: Summary• Cell line husbandry and
Page 27: Back-up slides

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