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Outline• Ion channel drug discovery at Pfizer– Brief description of resources and team• Infrastructure– Hardware investment– IonWorks applications in a drug discovery environment• Case Study– Na v Assay• Challenges/Issues
Groton ion channel drug discoveryCNS ion channel lab groupsGrotonAnn Arbor• Re-deployment from AA to Groton• Hardware upgrade to 48 channeldispensing head and fluidics25 lab members
Page 1: Realizing the potential of IonWorks
Page 5 and 6: Electrophysiology has an integralro
Page 7 and 8: Assessment of cell growth parameter
Page 9 and 10: Growth kinetics dictate experimenta
Page 11 and 12: Variables influencing assay success
Page 13 and 14: The importance of voltage control o
Page 15 and 16: Sample ion channel recordingPre-com
Page 17 and 18: Assay validation post-relocation•
Page 19 and 20: Resolution approachRe-chlorideE-hea
Page 21 and 22: I-V and inactivation curvesre-visit
Page 23 and 24: Pre- to post-addition current ampli
Page 25 and 26: Summary• Cell line husbandry and
Page 27 and 28: Back-up slides
Page 29 and 30: Kv70Single hole mode70Population pa

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