â Realizing the potential of IonWorks screening in a drug discovery ...

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What happened?• Run protocol withreference agents– Verify IC 50 valueshaven’t drifted-- Compound IC 50 values shifted from historical-- cell-cell variability unacceptably high-- run-down/run-up outside <strong>of</strong> allowable limits (± 5%)
Resolution approachRe-chlorideE-headThaw new cellsSteps takento mitigateissuesLiaise with MDCObta<strong>in</strong>freshreagentsPreparefreshsolutions
Page 1 and 2: Realizing the potential of IonWorks
Page 3 and 4: Groton ion channel drug discoveryCN
Page 5 and 6: Electrophysiology has an integralro
Page 7 and 8: Assessment of cell growth parameter
Page 9 and 10: Growth kinetics dictate experimenta
Page 11 and 12: Variables influencing assay success
Page 13 and 14: The importance of voltage control o
Page 15 and 16: Sample ion channel recordingPre-com
Page 17: Assay validation post-relocation•
Page 21 and 22: I-V and inactivation curvesre-visit
Page 23 and 24: Pre- to post-addition current ampli
Page 25 and 26: Summary• Cell line husbandry and
Page 27 and 28: Back-up slides
Page 29 and 30: Kv70Single hole mode70Population pa

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