â Realizing the potential of IonWorks screening in a drug discovery ...

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Voltage-dependence of compound xpharmacologyCompound x IC 50vs. Holding Potential80706050IC 50(µM)403020100-50mV-55mVHolding Potential (mV)-65mV•This figure demonstrates the voltage-dependence of compound xpharmacology.•Compound x pharmacology is used as a measure of voltage control ineach day’s run
Sample ion channel recordingPre-compoundPost-compoundIC 50 (#1)Use-Dependency Ratio:IC 50 (#1) / IC 50 (#25)IC 50 (#25)
Page 1 and 2: Realizing the potential of IonWorks
Page 3 and 4: Groton ion channel drug discoveryCN
Page 5 and 6: Electrophysiology has an integralro
Page 7 and 8: Assessment of cell growth parameter
Page 9 and 10: Growth kinetics dictate experimenta
Page 11 and 12: Variables influencing assay success
Page 13: The importance of voltage control o
Page 17 and 18: Assay validation post-relocation•
Page 19 and 20: Resolution approachRe-chlorideE-hea
Page 21 and 22: I-V and inactivation curvesre-visit
Page 23 and 24: Pre- to post-addition current ampli
Page 25 and 26: Summary• Cell line husbandry and
Page 27 and 28: Back-up slides
Page 29 and 30: Kv70Single hole mode70Population pa

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â Realizing the potential of IonWorks screening in a drug discovery ...