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Pipetting and dispensing29Summary of applicationsApplication Product PageManual pipettingInstrumentsSingle-channel, combined tip ejection and pipetting button Eppendorf Reference ® 38Single-channel, separate tip ejection button Eppendorf Research ® , Research plus 31Multichannel, separate tip ejection button Eppendorf Research ® , Research plus 31Single-channel, combined tip ejection and pipetting button,BioMaster ® Positive Displacement Pipette 58positive displacementSingle-channel, combined tip ejection and pipetting button,Maxipettor ® 59can be used as either a positive-displacement or an air-cushion pipetteElectronic pipetting, single-channel and multichannel,Eppendorf Xplorer ® 43separate tip ejection buttonPipet fillers for use with serological pipets Easypet ® /Pipet Helper ® 73ConsumablesStandard application epT.I.P.S. ® Bulk/Racks/Reloads 47Standard application, tips for Maxipettor Maxitip P, Maxitip S 59Contamination-free pipetting ep Dualfilter T.I.P.S. ® 56Contamination-free pipetting, tips for Positive Displacement Pipette Positive Displacement Pipette Tips 58Pipetting with the highest level of biological purityepT.I.P.S. Racks/Singles,47Eppendorf Biopur ®Applications with samples that contain detergents epT.I.P.S. LoRetention 50Manual dispensing (dispensing of partial volumes)InstrumentsManual dispensing, single-channel, positive displacement,Repeater ® Plus 65contamination-freeElectronic dispensing, single-channel, positive displacement,Repeater stream/Xstream 62contamination-freeElectronic dispensing, single-channel and multichannelEppendorf Xplorer 43separate tip ejection buttonConsumablesTips for Repeater Plus and Repeater stream/Xstream,contamination-free, standard application and highest level of biological purityCombitips Plus ® , Standard,Sterile and Biopur68Tips for Eppendorf XplorerContamination-freeService products for manual pipettingepT.I.P.S.ep Dualfilter T.I.P.S.Preventive Maintenance QuickCheck PM SM 100Pipette Calibration Advantage Calibration 100Pipette Calibration and Certification Advantage Certification 100Custom Calibration and Certification GLP/GMP Custom Calibration 100471 | Info | Pipetting | DispensingIn the U.S.: Eppendorf North America 800-645-3050 · In Canada: Eppendorf Canada Ltd. 800-263-8715E-mail: info@eppendorf.com · www.eppendorf.com

Adjustable- and fixed-volume pipettes31NEW! Eppendorf Research® plusNEW!1 | Instruments | Pipetting | DispensingEppendorf ® sets a new standard in pipetting. Theultra light Eppendorf Research plus pipette meets thehighest needs in precision and accuracy—combinedwith ultimate ergonomics and increased flexibility.It is a good feeling to know you are working with oneof the most advanced pipettes in the world. A springloaded tip cone, a second ary adjustment option, animproved volume display—and all that in an ultra light,fully autoclavable pipette: That is the new EppendorfResearch plus pipette.Ergonomics: Feel the difference in weight, pipetting forcesand the spring loaded tip cone.Flexibility: Your next experiment will need new pipettingdemands. Your new laboratory might have different sources ofcontamination. Your new pipette should offer all the flexibilityyou need. Adjust your Research plus to your needs, autoclavethe entire pipette or only the lower part. Choose among singlechannel,multichannel and fixed-volume pipettes in different sizes.Robustness: Durability was tested thoroughly and has met ourhighest quality requirements. Made with space-age materials,resistance to chemical, thermal and mechanical stress is nowcontinuously achieved!In the U.S.: Eppendorf North America 800-645-3050 · In Canada: Eppendorf Canada Ltd. 800-263-8715E-mail: info@eppendorf.com · www.eppendorf.com

32Adjustable- and fixed-volume pipettesNEW! Eppendorf Research® plus1 | Instruments | Pipetting | DispensingAn Eppendorf Research plus pipette is ofextremely low weight!This can only be achieved by using the most advancedmaterials and production processes, including the newEppendorf PerfectPiston system, made of Fortron ® .Fortron is an organic polymer with impressive characteristics.It is highly resistant to heat, acids and alkalies, mildew,bleaches, aging, sunlight and abrasion. It absorbs onlysmall amounts of solvents and resists dyeing. This meansyour pipette is safe, robust and ultra light.Eppendorf Research ® plusCompetitor BrCompetitor BiCompetitor GnCompetitor RCompetitor RL≤10 µl10–100 µl100–1000 µlCompetitor F50 g 60 g 70 g 80 g 90 g 100 g 110 g 120 g 130 g‡ See the low weight of the Research plus—no matter which size.Order from your laboratory supply dealer.Product appearance, specifications, and/or prices are subject to change without notice.

38 Adjustable- and fixed-volume pipettesReference® fixed-volume and adjustable-volume pipettes1 | Instruments | Pipetting | DispensingDescriptionThe patented 1 Reference pipette is synonymous with pipettingaccuracy, a smooth stroke, a compact and robust designand superior operating comfort. One multifunctional buttonoperates measuring stroke and blow-out as well as ejectsthe tip. There are 8 adjustable-volume models to cover therange from 0.1 µL to 2,500 µL.Packaged in 100% recyclable material; for more informationon epGreen products, see pages 376 and 377.Eppendorf epGreenEppendorf ® recognizes its responsibility as a global citizen andis striving to reduce the environmental impact of its businessoperations and the products we produce, as well as to supportthe sustainable green initiatives of our customers. In this sectionyou will find products with special epGreen features.For more information on epGreen,see pages 376 and 377.Order from your laboratory supply dealer.Product appearance, specifications, and/or prices are subject to change without notice.

40Adjustable- and fixed-volume pipettesReference® adjustable-volume pipettes1 | Instruments | Pipetting | DispensingProduct features‡‡Fully autoclavable‡‡One-button operation foraspiration, dispensing andtip ejection‡‡Volume settingautomatically locks‡‡Chemically resistant‡‡TÜV award: user friendlyand user-tested‡‡Single-handed operation‡‡4-digit display‡‡3-year warranty‡‡100% recyclablepackaging materialsOrdering information: Reference adjustable-volume pipettesAll Reference 1 pipettes can bereadjusted and calibratedfor use with liquids that have adifferent density than water.Each pipette contains aservice kit and an epT.I.P.S. ®Box with the appropriate tips.VolumeVolume range increment Volume Inaccuracy 2 Imprecision 2 Catalog No. List USDDark grey operating button, for use with 10 μL pipette tips0.1–2.5 µL 0.002 µL 0.25 µL ±12.0% ≤6.0% 022470001 $388.001.25 µL ±2.5% ≤1.5%2.5 µL ±1.4% ≤0.7%Grey operating button, for use with 20 μL pipette tips0.5–10 µL 0.01 µL 1 µL ±2.5% ≤1.8% 022470051 388.005 µL ±1.5% ≤0.8%10 µL ±1.0% ≤0.4%2–20 µL 0.02 µL 2 µL ±3.0% ≤2.0% 022470108 388.0010 µL ±1.0% ≤0.5%20 µL ±0.8% ≤0.3%Yellow operating button, for use with 200 μL and 300 μL pipette tips2–20 µL 0.02 µL 2 µL ±5.0% ≤1.5% 022470159 388.0010 µL ±1.2% ≤0.6%20 µL ±1.0% ≤0.3%10–100 µL 0.1 µL 10 µL ±3.0% ≤0.7% 022470205 388.0050 µL ±1.0% ≤0.3%100 µL ±0.8% ≤0.15%50–200 µL 0.2 µL 50 µL ±1.0% ≤0.3% 022470256 388.00100 µL ±0.9% ≤0.3%200 µL ±0.6% ≤0.2%Blue operating button, for use with 1,000 μL pipette tips100–1,000 µL 1 µL 100 µL ±3.0% ≤0.3% 022470302 388.00500 µL ±1.0% ≤0.2%1,000 µL ±0.6% ≤0.2%Red operating button, for use with 2,500 μL pipette tips500–2,500 µL 2 µL 500 µL ±1.5% ≤0.3% 022470353 388.001,000 µL ±0.8% ≤0.2%2,500 µL ±0.6% ≤0.2%Liquid: Bidistilled water, degassedTemperature: 20°C–25°C, constant to ±0.5 °CNo. of measurements: 10, in accordance with EN ISO 8655 using original Eppendorf ® pipette tips 1 U.S. Pat. 5,511,4332The data for imprecision (random measurement error) and inaccuracy (systematic measurement error) according to EN ISO 8655 is valid only when original Eppendorf pipette tips are used.Ordering information: AccessoriesDescription Catalog No. List USDPipette Carousel Stand, for six pipettes 022444905 $129.00Pipette Holder, for wall mounting 022444913 29.00Order from your laboratory supply dealer.Product appearance, specifications, and/or prices are subject to change without notice.

Adjustable- and fixed-volume pipettes41epReference 3 Pack1 | Instruments | Pipetting | DispensingepReference 3 Packs include:‡‡Option 1:3 Eppendorf ® Reference ® PhysioCare Concept ® pipettes(0.5–10 µL, 10–100 µL, 100–1,000 µL); 3 refillable epT.I.P.S. ®boxes with 96 tips each; 3 Pipette Holders; SOP andEppendorf pipette pen!‡‡Option 2:3 Eppendorf Reference PhysioCare Concept pipettes(2–20 µL, 50–200 µL, 100–1,000 µL); 3 refillable epT.I.P.S.boxes with 96 tips each; 3 Pipette Holders; SOP andEppendorf pipette pen!DescriptionComfortable, high precision pipetting is the result of perfectteamwork between the pipette and tip. Why not rely uponthe unbeatable team of Eppendorf Reference pipettesand epT.I.P.S. pipette tips? Now conveniently available asepReference 3 Pack at a special price that’s too good to miss!Packaged in 100% recyclable material; for more informationon epGreen products, see pages 376 and 377.Ordering informationDescription Catalog No. List USDepReference 3 Pack 1, (10, 100 and 1,000 µL pipettes) 022470388 $876.00epReference 3 Pack 2, (20, 200 and 1,000 µL pipettes) 022470396 876.00In the U.S.: Eppendorf North America 800-645-3050 · In Canada: Eppendorf Canada Ltd. 800-263-8715E-mail: info@eppendorf.com · www.eppendorf.com

42Electronic pipettesEppendorf® pipettes accessories1 | Instruments | Pipetting | DispensingNEW!DescriptionEnjoy an organized workplace with our rotating pipette stand.Pipettes attach to the stand by the handle, not the nose cone,which rules out potential cross-contamination from the standto the pipettes.In addition to the Eppendorf Research ® and Reference ® pipettes,the stand can also hold all older models of Eppendorf pipettes.Also available: a pipette holder that attaches to the wall, whichkeeps the pipette within reach, even when you have limitedbench space.DescriptionThe charging rack for the Eppendorf Xplorer ® enables you tocharge your electronic pipette in an uncomplicated fashion whileat the same time avoiding contamination by improper storage. Inaddition, the quadruple charging rack allows you to charge yourRepeater Stream/Xstream on the same rack using an adapter.Product features‡‡Pipette carousel for 6 pcs, Reference and Research plus‡‡With an adapter, also suitable for use with Repeater ® plusand Maxipettor ®For pipette service and maintenance information, see page 100;pipette tips information begins on page 47.Ordering informationDescription Catalog No. List USDPipette carousel022444905 $129.00for 6 pipettesPipette holder for wall mounting 022444913 29.00Repeater Plus/ Maxipettor 022261584 18.00adapter for pipette carouselstandRepeater Plus/ Maxipettor 022260596 29.00adapter for wall mountingCharging rack for 1 Xplorer 4880000000 231.00Charging rack for 4 Xplorer 4880000026 681.00Order from your laboratory supply dealer.Product appearance, specifications, and/or prices are subject to change without notice.

Electronic pipettes45NEW! Eppendorf Xplorer® electronic pipettes‡ Selection dial– All functions at a glanceand easily selectable1 | Instruments | Pipetting | Dispensing‡ Separate power socket– Work continues while theXplorer is charging‡ Practical charging contacts– Pipette can also be chargedwith the charging stand‡ Modern color display– Self-explanatory with allparameters—without submenus‡ Multilingual menu navigation– Various languages available forselection‡ Powerful battery– Long service life without charging‡ Ergonomic display angle– Optimal readability in everypositionPipette tips information begins on page 47.Ordering information for other voltage optionsbegins on page 46.In the U.S.: Eppendorf North America 800-645-3050 · In Canada: Eppendorf Canada Ltd. 800-263-8715E-mail: info@eppendorf.com · www.eppendorf.com

46Electronic pipettesEppendorf Xplorer® electronic pipettes1 | Instruments | Pipetting | DispensingOrdering information: Eppendorf Xplorer electronic pipettesModelVolumerange Volume1Systematic error*1Random error* Catalog No. List USDSingle-channel pipetteMedium grey multi-function rocker for 20 μL pipette tipswith charging adapter 0.5–10 µL 1 µL ±2.5% ±0.025 µL ±1.8% ±0.018 µL 4861000015 $ 688.005 µL ±1.5% ±0.075 µL ±0.8% ±0.04 µL10 µL ±1.0% ±0.1 µL ±0.4% ±0.04 µLYellow multi-function rocker for 200 μL pipette tipswith charging adapter 5–100 µL 10 µL ±2.0% ±0.2 µL ±1.0% ±0.1 µL 4861000023 688.0050 µL ±1.0% ±0.5 µL ±0.3% ±0.15 µL100 µL ±0.8% ±0.8 µL ±0.2% ±0.2 µLOrange multi-function rocker for 300 μL pipette tipswith charging adapter 15–300 µL 30 µL ±2.5% ±0.75 µL ±0.7% ±0.21 µL 4861000031 688.00150 µL ±1.0% ±1.5 µL ±0.3% ±0.45 µL300 µL ±0.6% ±1.8 µL ±0.2% ±0.6 µLBlue multi-function rocker for 1,000 μL pipette tipswith charging adapter 50–1,000 µL 100 µL ±3.0% ±3 µL ±0.6% ±0.6 µL 4861000040 688.00500 µL ±1.0% ±5 µL ±0.2% ±1 µL1,000 µL ±0.6% ±6 µL ±0.2% ±2 µLViolet multi-function rocker for 5 mL pipette tipswith charging adapter 0.25–5 mL 500 µL ±3.0% ±15 µL ±0.6% ±3 µL 4861000058 688.002,500 µL ±1.2% ±30 µL ±0.3% ±6.25 µL5,000 µL ±0.6% ±30 µL ±0.15% ±7.5 µLTurquoise multi-function rocker for 10 mL pipette tipswith charging adapter 0.5–10 mL 1,000 µL ±3.0% ±30 µL ±0.6% ±6 µL 4861000066 688.005,000 µL ±0.8% ±40 µL ±0.2% ±10 µL10,000 µL ±0.6% ±60 µL ±0.15% ±15 µLMultichannel pipette incl. charging adapterMedium grey multi-function rocker for 20 μL pipette tips8-channel 0.5–10 µL 1 µL ±5.0% ±0.05 µL ±3.0% ±0.03 µL 4861000104 1,172.005 µL ±3.0% ±0.15 µL ±1.5% ±0.075 µL10 µL ±2.0% ±0.2 µL ±0.8% ±0.08 µL12-channel 4861000112 1,482.00Yellow multi-function rocker for 200 μL pipette tips8-channel 5–100 µL 10 µL ±2.0% ±0.2 µL ±2.0% ±0.2 µL 4861000120 1,172.0050 µL ±1.0% ±0.5 µL ±0.8% ±0.4 µL100 µL ±0.8% ±0.8 µL ±0.25% ±0.25 µL12-channel 4861000139 1,482.00Orange multi-function rocker for 300 μL pipette tips8-channel 15–300 µL 30 µL ±2.5% ±0.75 µL ±1.0% ±0.3 µL 4861000147 1,172.00150 µL ±1.0% ±1.5 µL ±0.5% ±0.75 µL300 µL ±1.8% ±1.8 µL ±0.25% ±0.75 µL12-channel 4861000155 1,482.00Green multi-function rocker for 1,200 μL pipette tips8-channel 50–1,200 µL 120 µL600 µL1,200 µL±6.0%±2.7%±1.2%±7.2 µL±16.2 µL±14.4 µL±0.9%±0.4%±0.3%±1.08 µL±2.4 µL±3.6 µL*1 The data for systematic error and random error according to EN ISO 8655 is valid only when original Eppendorf ® pipette tips are used.4861000163 1,172.00Order from your laboratory supply dealer.Product appearance, specifications, and/or prices are subject to change without notice.

48Pipette tipsHandling the reload system—Contamination Free!TIPS1 | Consumables | Pipetting | DispensingLiftLiftThe transparent blue protective lid for epT.I.P.S. ® Reloads allows individual trays to be lifted off of the respective stack (left) ordual tray (right) without contamination.TransferEngageAn audible “click” indicates that the tray is fully engaged. The blue lid can be used to reseal the epT.I.P.S. Reloads to protect the tipsfrom contamination.Order from your laboratory supply dealer.Product appearance, specifications, and/or prices are subject to change without notice.

Pipette tips49Eppendorf® purity levels for tips, Combitips®, tubes and platesEppendorfQualityContinuous quality control for the following quality criteria:EppendorfSterileEppendorfPCR cleanEppendorfPCR clean,sterile and freeof pyrogensEppendorfBiopur ®Function, tightness, precision ‡ ‡ ‡ ‡ ‡Defined flow dynamics ‡ ‡ ‡ ‡ ‡Minimal water retention ‡ ‡ ‡ ‡ ‡High chemical resistance ‡ ‡ ‡ ‡ ‡High thermal stability ‡ ‡ ‡ ‡ ‡Precisely molded ‡ ‡ ‡ ‡ ‡Batch testing 1 (certified) for the following purity criteria:Free of human DNA ‡ ‡ ‡Free of bacteria DNA (E. coli) ‡DNase-free ‡ ‡RNase-free ‡ ‡ ‡Free of PCR inhibitors ‡ ‡ATP-free ‡Pyrogen-free (endotoxin-free) ‡ ‡Sterile (Ph.Eur./USP) ‡ ‡ ‡Eppendorf productsTipsepT.I.P.S. ® Standard (Bulk) ‡epT.I.P.S. Box ‡epT.I.P.S. Reloads ‡ ‡epT.I.P.S. Starter Set ‡epT.I.P.S. Racks ‡ ‡ ‡ ‡epT.I.P.S. Singles (individually packaged) ‡ ‡ep Dualfilter T.I.P.S. ® ‡Combitips Plus tips ‡ ‡ ‡ 2epT.I.P.S. LoRetention ‡ ‡ ‡epT.I.P.S. Motion ‡ ‡epT.I.P.S. Motion filtertips ‡ ‡TubesSafe-Lock tubes ‡ ‡ ‡ 2Flex-Tube ® ‡ ‡Protein LoBind tubes ‡DNA LoBind tubes ‡PCR Tubes (PCR and qPCR) ‡Cap Strips (PCR and qPCR) ‡Plates and accessoriesDeepwell Plates ‡ ‡Protein LoBind Deepwell Plates ‡DNA LoBind Deepwell Plates ‡twin.tec PCR Plates (PCR and qPCR) ‡Self-adhesive sealing materials ‡Heat sealing materials ‡Capping Aid ‡in situ Frame ‡1 | Consumables | Pipetting | Dispensing1Batch-specific certificates can be downloaded from www.eppendorfna.com/certificates2Individually packaged.For more information on Eppendorf purity levels, see pages372 to 375, or visit www.eppendorfna.com/purity.In the U.S.: Eppendorf North America 800-645-3050 · In Canada: Eppendorf Canada Ltd. 800-263-8715E-mail: info@eppendorf.com · www.eppendorf.com

50Pipette tipsepT.I.P.S.® LoRetention1 | Consumables | Pipetting | Dispensing21Simple differentiation of racks and reloads througha new, clear lid with a white seal. When open, thewriting “LoRetention” can be seen from the inside.The reusable seal also makes it possible to labelexisting epT.I.P.S. boxes.The “Pearl Effect”—a comparison of the amount ofresidual liquid when pipetting solutions that containdetergents: Maximum recovery with epT.I.P.S.LoRetention Significant loss of sample using standard tipsProduct features‡‡Ultrahomogeneous surface for maximum reproducibility‡‡Ultrahydrophobic surface for minimum loss of sample‡‡Air bubbles and foaming are significantly reducedduring pipetting‡‡Autoclavable, high chemical resistance‡‡5-year shelf life‡‡Available as ep Dualfilter T.I.P.S. ® , as well as inPCR clean and Eppendorf QualityMaximum resultsThe ultrahydrophobic, extremely homogenous surfaceof the epT.I.P.S. LoRetention is achieved through an innovativetreatment at the molecular level—the Pearl Effect Technology.The tips are uncoated and free from additives, thus nothingcan bleed into the sample. Liquids roll off completely, sothat almost no sample remains in the tip. In comparison, theadhesive layer of liquid in standard tips retains considerablymore sample material.Reduce costs, increase reproducibilityParticularly with sensitive PCR and real-time PCRapplications, low-retention surfaces can increasereproducibility and notably reduce the loss ofexpensive reagents—depending on the reagentsolution by up to 5%!Various applicationsepT.I.P.S. LoRetention are ideally suited for allapplications with samples that contain detergents:‡ PCR and real-time PCR preparation of enzymesolutions and master mixes‡ Isolation, purification and denaturation of proteins‡ Restriction digestions and ligations‡ Pipetting DNA ladders during gel electrophoresisepT.I.P.S. LoRetention perform considerably betterin terms of precision and sample recovery thanlow-retention tips from other manufacturers.This unique material property guarantees maximum samplerecovery, improved reproducibility and higher sensitivity.Order from your laboratory supply dealer.Product appearance, specifications, and/or prices are subject to change without notice.

Pipette tips51epT.I.P.S.® LoRetentionQuality and purity Eppendorf ® styleIt goes without saying that the new epT.I.P.S. LoRetention haveall the valued properties of the proven Eppendorf epT.I.P.S.They are produced in high-precision manu facturing processes,can be autoclaved, are extremely resistant to chemicals, and areResidual liquid [µl]6.05.04.03.02.00.1% Triton X-100PCR-Mastermixavailable in the PCR clean (free of human DNA, DNase, RNase,and PCR inhibitors) purity grade, as well as in EppendorfQuality. For maximum contamination protection, epT.I.P.S.LoRetention are also available as ep Dualfilter T.I.P.S. ®Residual liquid [µl]6.05.04.03.02.0ep T.I.P.S. LoRetentionLow-Retention Competitor ALow-Retention Competitor M1 | Consumables | Pipetting | Dispensing1.01.00.0epT.I.P.SLoRetentionCompetitor ALow-RetentionCompetitor MLow-RetentionCompetitor SLow-Retention0.0Untreated Acetonitrile Acetone Isopropanol AutoclavedFig. 1. Comparison of residual liquid from epT.I.P.S.LoRetention and low-retention tips of other manufacturers.200 µL tips were used to pipette a commercial PCR master mixand the detergent 0.1% Triton X-100. Once the liquid had beendispensed, the residual liquid in the tips was determined. Themeasurements were repeated several times and the standarddeviation determined. epT.I.P.S. LoRetention resulted in thelowest residual liquid, as well as the smallest standard deviation.Fig. 2. Comparison of resistance of the low-retentionproperty when subjected to solvents or autoclaving.200 µL epT.I.P.S. LoRetention and low-retention tips of othermanufacturers were autoclaved or treated with the solventspecified. An enzymatic buffer containing detergents was thenpipetted and the residual moisture was determined. epT.I.P.S.LoRetention resulted in reproducible low levels of residual liquidwith minimum standard deviation. Low-retention tips from othermanufacturers are less resistant to chemicals or autoclaving: thisresulted in an increase in residual liquid and standard deviation.Ordering informationDescription Catalog No. List USDepT.I.P.S. LoRetention Dualfilter,Racks, PCR clean/sterile/pyrogen-free, 10 × 96 tips0.1–10 µL Short 022493000 $144.000.5–20 µL Long 022493002 144.002–100 µL 022493006 134.0020–300 µL 022493004 144.0050–1,000 µL 022493008 144.00epT.I.P.S LoRetention, Reloads, PCR clean, 10 × 96 tips0.1–10 µL Short 022493010 98.000.5–20 µL Long 022493012 98.002–200 µL 022493014 80.0050–1,000 µL 022493016 86.00Description Catalog No. List USDepT.I.P.S LoRetention,Reloads, Eppendorf Quality, 10 × 96 tips0.1–10 µL Short 022493018 $ 86.000.5–20 µL Long 022493020 86.002–200 µL 022493022 68.0050–1,000 µL 022493024 75.00epT.I.P.S LoRetention, Racks, PCR clean, 10 × 96 tips0.1–10 µL Short 022493026 119.000.5–20 µL Long 022493028 119.002–200 µL 022493030 95.0050–1,000 µL 022493032 102.00For more information on Eppendorf purity levels, see pages372 to 375, or visit www.eppendorfna.com/purity.In the U.S.: Eppendorf North America 800-645-3050 · In Canada: Eppendorf Canada Ltd. 800-263-8715E-mail: info@eppendorf.com · www.eppendorf.com

52Pipette tipsPipette tipsTIPS1 | Consumables | Pipetting | DispensingVolume range/pipette tip(all tips shown are actual size Standard Reloads Reloadsand are colourless unlessotherwise stated)Eppendorf Quality Eppendorf Quality PCR clean0.1–10 µL, 34 mm 2 bags of 500 tips = 1,000 tips 10 trays of 96 tips = 960 tips *1 10 trays of 96 tips = 960 tips *1022492004 $63.00 022491504 $78.00 022491709 $90.000.1–20 µL, 40 mm 2 bags of 500 tips = 1,000 tips 10 trays of 96 tips = 960 tips 10 trays of 96 tips = 960 tips022492012 $63.00 022491512 $78.00 022491717 $90.000.5–20 µL L, 46 mm 2 bags of 500 tips = 1,000 tips 10 trays of 96 tips = 960 tips 10 trays of 96 tips = 960 tips022492021 $63.00 022491521 $78.00 022491725 $90.002–200 µL, 53 mm 2 bags of 500 tips = 1,000 tips 10 trays of 96 tips = 960 tips* 1 10 trays of 96 tips = 960 tips* 1022492039 $51.000030000870 yellow 022491539 $60.00 022491733 $72.0020–300 µL, 55 mm 2 bags of 500 tips = 1,000 tips 10 trays of 96 tips = 960 tips 10 trays of 96 tips = 960 tips022492047 $51.000030000897 yellow 022491547 $60.00 022491741 $72.0050–1,000 µL, 71 mm 2 bags of 500 tips = 1,000 tips 10 trays of 96 tips = 960 tips 10 trays of 96 tips = 960 tips022492055 $54.000030000919 blue 022491555 $67.00 022491750 $78.0050–1,250 µL, 76 mm 2 bags of 500 tips = 1,000 tips 10 trays of 96 tips = 960 tips 10 trays of 96 tips = 960 tips022492063 $54.00 022491563 $67.00 022491768 $78.00Note: All pipette tips meet the Eppendorf Quality purity standard ; where applicable, higher purity levels are noted.*1 Packed in the form of a stack that includes five reloads. All nonmarked epT.I.P.S. ® Reloads are packed dual-sided.For more information go to www.eppendorfna.com/consumablesOrder from your laboratory supply dealer.Product appearance, specifications, and/or prices are subject to change without notice.

Pipette tips 53Pipette tipsTIPSBox Set Singles RacksEppendorf Quality Eppendorf Quality Eppendorf Biopur ® (sterile) Eppendorf Biopur (sterile)1 reusable box incl. 96 tips 1 reusable box incl. 5 trays of96 tips1 | Consumables | Pipetting | Dispensing022491300 $35.00 022491407 $63.001 reusable box incl. 96 tips 1 reusable box incl. 5 trays of96 tips100 tips, individually wrapped 5 racks of 96 tips = 480 tips022491318 $35.00 022491415 $63.00 022491130 $29.00 022491067 $89.001 reusable box incl. 96 tips 1 reusable box incl. 5 trays of96 tips022491326 $35.00 022491423 $63.001 reusable box incl. 96 tips 1 reusable box incl. 5 trays of96 tips100 tips, individually wrapped 5 racks of 96 tips = 480 tips022491334 $35.00 022491431 $51.00 022491148 $24.00 022491083 $82.001 reusable box incl. 96 tips 1 reusable box incl. 5 trays of96 tips5 racks of 96 tips = 480 tips022491342 $35.00 022491490 $51.00 022491091 $82.001 reusable box incl. 96 tips 1 reusable box incl. 5 trays of96 tips100 tips, individually wrapped 5 racks of 96 tips = 480 tips022491351 $35.00 022491458 $54.00 022491156 $25.001 reusable box incl. 96 tips 1 reusable box incl. 5 trays of96 tips5 racks of 96 tips = 480 tips022491369 $35.00 022491466 $54.00 022491113 $88.00In the U.S.: Eppendorf North America 800-645-3050 · In Canada: Eppendorf Canada Ltd. 800-263-8715E-mail: info@eppendorf.com · www.eppendorf.com

54 Pipette tipsPipette tipsTIPS1 | Consumables | Pipetting | DispensingStandard Reloads ReloadsVolume range/pipette tip(most tips shown are actual size) Eppendorf Quality Eppendorf Quality PCR clean50–1,250 µL L, 103 mm 2 bags of 500 tips = 1,000 tips 10 trays of 96 tips = 960 tips 10 trays of 96 tips = 960 tips022494018 $55.00 022494004 $70.00 022494006 $80.00500–2,500 µL, 115 mm 5 bags of 100 tips = 500 tips 10 trays of 48 tips = 480 tips 10 trays of 48 tips = 480 tips022492071 $63.00 022491571 $67.00 022491776 $78.00100–5,000 µL, 120 mm 5 bags of 100 tips = 500 tips022492080 $87.001–10 mL, 165 mm 2 bags of 100 tips = 200 tips1–10 mL L, 243 mm(Image reduced in size)022492098 $49.002 bags of 100 tips = 200 tips Fits in deep and narrow test tubes, cell culture bottles andmeasuring cylinders022492101 $55.00For more information go to www.eppendorfna.com/consumablesOrder from your laboratory supply dealer.Product appearance, specifications, and/or prices are subject to change without notice.

Pipette tips55Pipette tipsTIPSBox Set Singles RacksEppendorf Quality Eppendorf Quality Eppendorf Biopur ® (sterile) Eppendorf Biopur (sterile)1 reusable box incl. 96 tips 5 racks of 96 tips = 480 tips1 | Consumables | Pipetting | Dispensing022494016 $33.00 022494014 $48.001 reusable box incl. 48 tips 1 reusable box incl. 5 trays of48 tips5 racks of 48 tips = 240 tips022491377 $35.00 022491474 $64.00 022491121 $88.001 reusable box incl. 24 tips022491385 $35.005 racks of 24 tips = 120 tips022491164 $76.00In the U.S.: Eppendorf North America 800-645-3050 · In Canada: Eppendorf Canada Ltd. 800-263-8715E-mail: info@eppendorf.com · www.eppendorf.com

56Pipette tipsPipette tips1 | Consumables | Pipetting | DispensingVolume range/pipette tip(all tips shown are actual size)DualfilterPCR clean, sterile and pyrogen free0.1–10 µL S, 34 mm 10 racks of 96 tips = 960 tips022491202 $136.000.1–10 µL M, 40 mm 10 racks of 96 tips = 960 tips022491211 $136.000.5–20 µL L, 46 mm 10 racks of 96 tips = 960 tips022491229 $136.002–20 µL, 53 mm 10 racks of 96 tips = 960 tips022491270 $136.002–100 µL, 53 mm 10 racks of 96 tips = 960 tips022491237 $126.002–200 µL, 55 mm 10 racks of 96 tips = 960 tips022491296 $126.0020–300 µL, 55 mm 10 racks of 96 tips = 960 tips022491245 $126.0050–1,000 µL, 76 mm 10 racks of 96 tips = 960 tips022491253 $136.00For more information go to www.eppendorfna.com/consumablesOrder from your laboratory supply dealer.Product appearance, specifications, and/or prices are subject to change without notice.

Pipette tips57Pipette tipsVolume range/pipette tip(most tips shown are actual size)DualfilterPCR clean, sterile and pyrogen free50–1,250 µL L, 103 mm 5 Racks of 96 Tips = 480 Tips1 | Consumables | Pipetting | Dispensing022494002 $71.00100–5,000 µL, 120 mm 5 racks of 24 tips = 120 tips1–10 mL L, 243 mm(Image reduced in size)022491261 $75.00100 tips, individually wrapped022491288 $63.00Note: All pipette tips meet the Eppendorf Quality purity standard; where applicable, higher purity levels are noted.Special tipsTIPSVolume range/pipette tip GELoader ®0.5–20 µL, 62 mm 2 racks of 96 tips = 192 tips022351656 $123.00Microloader0.5–20 µL, 100 mm 2 racks of 96 tips = 192 tips930001007 $150.00In the U.S.: Eppendorf North America 800-645-3050 · In Canada: Eppendorf Canada Ltd. 800-263-8715E-mail: info@eppendorf.com · www.eppendorf.com

60 Handheld dispensersElectronic and manual Repeater® Plus and stream/Xstream1 | Instruments | Pipetting | DispensingThe instrumentsThe manual Repeater Plus 1 and the electronic Repeaterstream/Xstream hand dispensers are ideal for processacceleration and saving time on long-series pipetting: theirincreased volume selection provides outstanding flexibilityand exceptional support for research as well as routine tasks;and their smooth handling reduces the fatigue associatedwith repetitive work.The consumablesCombitips Plus ® tips 2 easily attach to the Repeaters; their built-insensor recognizes the size of the tip, and the volume appearsautomatically in the display—eliminating time-consuming volumecalculations and incorrect dispensing volumes. The nine sizesof Combitips Plus allow a wider dispensing range of up to112 different volumes and longer series dispensing (e. g., 100 x250 µL) without stopping for a refill. Always contamination-free!A wide variety of solutionsCombitips Plus tips function according to the positivedisplacement principle, which ensures that the correct volumeis dispensed regardless of the density or viscosity of the liquid.The positive displacement system provides safer dispensing ofsolutions that contain radioactive and/or toxic substances, aswell as solutions that have a high vapor pressure, becauseno aerosols are formed. Combitips are easily replaced—ejectwith the push of a button—and dispose after use, alwayscontamination-free.1U.S. Patents 5,620,660/5,620,661/5,591,405/5,573,7292U.S. Patents 5,620,660/5,620,661Order from your laboratory supply dealer.Product appearance, specifications, and/or prices are subject to change without notice.

62 Electronic pipettesElectronic Repeater® stream and Repeater Xstream handheld dispensers1 | Instruments | Pipetting | Dispensing‡ Switch selection wheel to bold– Easily toggle between functions‡ Dispense monitoring– Real-time countdown ofdispensings displayed‡ Vibrant screen– Illuminated displayfor easy viewing‡ Effortless dispensing– Rapid, ergonomic dispensingwith motorized piston‡ Continuous volume selection– Dispensing from 1 µL to 50 mLBenefit from the high quality ofEppendorf epT.I.P.S. ® pipette tips(More informationwww.eppendorfna.com/consumables)‡ Combitip sensor– Automatic Combitips Plus ®tip recognitionOrder from your laboratory supply dealer.Product appearance, specifications, and/or prices are subject to change without notice.

Pipette stand63Electronic Repeater® stream and Repeater Xstream handheld dispensers‡ Flexible power options– Charging while in use is possible1 | Instruments | Pipetting | Dispensing‡ Intuitive programming menu– Electronic dispensing withprogrammable memory functions‡ Ergonomic handle– One-button tip ejector‡ Sterile Combitip plus– Contamination-freedispensing using positivedisplacement principle‡ Integrated piston– Ideal for viscous solutions(e.g. glycerol, oil) or liquidswith high vapor pressure(e.g. acetone)Combitips Plus ® tips information begins on page 60.In the U.S.: Eppendorf North America 800-645-3050 · In Canada: Eppendorf Canada Ltd. 800-263-8715E-mail: info@eppendorf.com · www.eppendorf.com

64 Manual and electronic dispensersElectronic Repeater® stream and Repeater Xstream handheld dispensers1 | Instruments | Pipetting | DispensingIlluminated display for easy viewing.Product features‡‡Electronic dispensing with programmablememory functions‡‡Contamination-free dispensing using thepositive displacement principle‡‡Dispensing from 1 μL to 50 mL‡‡Dispensing volumes in long series‡‡See pages 61–63 for additional featuresDescriptionElectronic Repeater stream and Xstream combine easy handlingwith a broad range of features. When used with Combitips Plus ® *tips (pages 68–71), the dispenser/tip forms an unbeatablesystem that is the quintessential lab tool for all applications:Repeater stream is suitable for all standard pipetting anddispensing applications; for added versatility, the RepeaterXstream can also perform titrations, sequential dispensingand aspiration of super natants.* U.S. Patents 5,620,660/5,620,661For technical specifications, see page 69.Packaged in 100% recyclable material; for more informationon epGreen products, see pages 376 and 377.Ordering informationDescription Catalog No. List USDRepeater stream022460803 $ 897.00electronic dispenserRepeater Xstreamelectronic dispenser022460811 1,077.00Order from your laboratory supply dealer.Product appearance, specifications, and/or prices are subject to change without notice.

Manual and electronic dispensers65Manual Repeater® Plus handheld dispenser1 | Instruments | Pipetting | DispensingThe rounded, grooved grip of the Repeater Plus means easierdispensing with less stress on the hand and thumb muscles.Product features‡‡Dispensing volumes in long series‡‡Contamination-free dispensing using thepositive displacement principle‡‡Ideal for viscous solutions (e.g., glycerol, oil) or liquidswith high vapor pressure (e.g., acetone, ethanol)‡‡Easy to read: automatic Combitips Plus ® recognitionwith electronic volume display‡‡Easy volume setting‡‡More flexible with 112 different volumes from1 µL to 10 mL‡‡Up to 100 dispensings per filling for easy processingof long series‡‡Versatile, even for “uneven” volumes, e.g., 1 µL, 3 µLor 17 µL‡‡Safe, due to easy, contact-free Combitips Plusattachment and ejection‡‡2-year warranty‡‡See pages 61 and 66–67 for additional featuresDescriptionRepeater Plus 1 is the ideal precision instrument for reducingthe time associated with manual, long-series pipetting.Its increased volume selection provides outstanding flexibilityand all-around support for research and routine tasks.The electronic display ensures error-free dispensing, andthe rounded, grooved grip makes dispensing easier andergonomic—with less stress on the hand and thumb muscles.1U.S. Pats. 5,620,660/5,620,661/5,591,405/5,573,729Ordering informationDescription Catalog No. List USDRepeater Plus022260201 $492.00manual dispenserPackaged in 100% recyclable material; for more informationon epGreen products, see pages 376 and 377.In the U.S.: Eppendorf North America 800-645-3050 · In Canada: Eppendorf Canada Ltd. 800-263-8715E-mail: info@eppendorf.com · www.eppendorf.com

Manual dispenser67Manual Repeater® Plus handheld dispenser‡ Volume selection wheel– Easy volume setting‡ Versatile– Even for “uneven” volumes(e.g. 1 µL, 3 µL or 17 µL)1 | Instruments | Pipetting | Dispensing‡ Digital display– Automatic Combitips ®recognition with electronicvolume display‡ Flexible volume selection– 20 different dispensing volumesper each Combitip size‡ Ergonomic tip release– Contact-free Combitipattachment and ejectionCombitips Plus ® tips information begins on page 60.In the U.S.: Eppendorf North America 800-645-3050 · In Canada: Eppendorf Canada Ltd. 800-263-8715E-mail: info@eppendorf.com · www.eppendorf.com

68 Manual and electronic dispensersCombitips Plus® tips and Repeater® Plus, stream and Xstream accessories1 | Consumables | Pipetting | DispensingProduct features of Combitips Plus‡‡Ideal system component for all Repeater, Repeater Plusand Repeater stream/Xstream (electronic)‡‡20 different dispensing volumes for each Combitip size(Repeater Plus)‡‡Several hundred different volumes for each Combitip size(Repeater stream/Xstream)‡‡Higher throughput with up to 100 dispensings per filling‡‡Reliable results ensured by positive displacement system‡‡Available in Eppendorf Quality, Sterile and EppendorfBiopur ® purity levels‡‡Available in 9 different sizes from 0.1 mL to 50 mL‡‡Certified trace metal releaseTip purity is ensuredIn addition to standard purity, Combitips Plus tips are availablein Sterile and Eppendorf Biopur. Eppendorf Biopur (page 49),signifying outstanding biological purity, guarantees that thesetips are sterile as well as pyrogen-, RNase-, DNase and ATPfree.They meet the most stringent demands of the medical,pharmaceutical and food industries, PCR applications andmolecular biology/cell technology labs. This purity isensured through an automated manufacturing process,which safeguards the product against contamination withbiological substances. Individual lot certificates are availableat www.eppendorfna.com/certificates.Wall mount Pipette stand Charging standAccessoriesAs a safeguard against potential contamination, our carouselstand does not hold the pipette at the bottom—like most otherstands do. Eppendorf Repeater, Repeater Plus and Maxipettor ®pipettes easily fit on the carousel with an adapter. To store theelectronic Repeater stream/Xstream, a charging stand isavailable (sold separately). Is your bench space at a premium?We also offer the practical wall-mounted pipette bracket—itkeeps your Repeater Plus off the bench but still within reach.Ordering informationDescription Catalog No. List USDCharging stand, for Repeater 022460897 $197.00stream/Xstream, holds one unitStand for Repeater pipettes, 022260651 65.00holds two unitsRepeater Plus adapter, for new 022260588 18.00pipette carousel standRepeater Plus Wall Mount 022260596 29.00Order from your laboratory supply dealer.Product appearance, specifications, and/or prices are subject to change without notice.

Manual and electronic dispensers69Combitips Plus® for Repeater® Plus and Repeater stream/XstreamTechnical specificationsVolume Increment/ Max. dispensingCombitips Plus min./max. step size volume Volume Inaccuracy 1 Imprecision 1For Repeater Plus 2 and Combitips Plus 3 system0.1 mL 1 µL 1 µL 20 µL 2 µL ±1.6% ≤3.0%20 µL 20 µL ±1.0% ≤2.0%0.2 mL 2 µL 2 µL 40 µL 4 µL ±1.3% ≤2.0%40 µL 40 µL ±0.8% ≤1.5%0.5 mL 5 µL 5 µL 100 µL 10 µL ±0.9% ≤1.5%100 µL 100 µL ±0.8% ≤0.6%1 mL 10 µL 10 µL 200 µL 20 µL ±0.9% ≤0.9%200 µL 200 µL ±0.6% ≤0.4%2.5 mL 25 µL 25 µL 500 µL 50 µL ±0.8% ≤0.8%500 µL 500 µL ±0.5% ≤0.3%5 mL 50 µL 50 µL 1,000 µL 100 µL ±0.6% ≤0.6%1,000 µL 1,000 µL ±0.5% ≤0.25%10 mL 100 µL 100 µL 2,000 µL 200 µL ±0.5% ≤0.6%2,000 µL 2,000 µL ±0.5% ≤0.25%25 mL 250 µL 250 µL 5,000 µL 500 µL ±0.4% ≤0.6%5,000 µL 5,000 µL ±0.3% ≤0.25%50 mL 500 µL 500 µL 10,000 µL 1,000 µL ±0.3% ≤0.5%10,000 µL 10,000 µL ±0.3% ≤0.25%For Repeater stream/Xstream and Combitips Plus system0.1 mL 1 µL 0.1 µL 0.1 mL 10 µL ±1.6% ≤2.5%100 µL 100 µL ±1.0% ≤0.5%0.2 mL 2 µL 0.2 µL 0.2 mL 20 µL ±1.3% ≤1.5%200 µL 200 µL ±1.0% ≤0.5%0.5 mL 5 µL 0.5 µL 0.5 mL 50 µL ±0.9% ≤0.8%500 µL 500 µL ±0.9% ≤0.3%1 mL 10 µL 1 µL 1 mL 100 µL ±0.9% ≤0.55%1 mL 1,000 µL ±0.6% ≤0.2%2.5 mL 25 µL 2.5 µL 2.5 mL 250 µL ±0.8% ≤0.45%2.5 mL 2,500 µL ±0.5% ≤0.15%5 mL 50 µL 5 µL 5 mL 500 µL ±0.8% ≤0.35%5 mL 5,000 µL ±0.5% ≤0.15%10 mL 100 µL 10 µL 10 mL 1,000 µL ±0.5% ≤0.25%10 mL 10,000 µL ±0.4% ≤0.15%25 mL 250 µL 25 µL 25 mL 2,500 µL ±0.3% ≤0.25%25 mL 25,000 µL ±0.3% ≤0.15%50 mL 500 µL 50 µL 50 mL 5,000 µL ±0.3% ≤0.25%50 mL 50,000 µL ±0.3% ≤0.15%1 | Consumables | Pipetting | Dispensing1The data for imprecision (random measurement error) and inaccuracy (systematic measurement error) according to EN ISO 8655 is valid only when original Eppendorf ® Combitips Plus are used.2U.S. Pats. 5,620,660/5,620,661/5,591,408/5,573,7293U.S. Pats. 5,620,660/5,620,661For ordering information, see next page.In the U.S.: Eppendorf North America 800-645-3050 · In Canada: Eppendorf Canada Ltd. 800-263-8715E-mail: info@eppendorf.com · www.eppendorf.com

70 Manual and electronic dispensersCombitips Plus®1 | Consumables | Pipetting | DispensingVolume table for using Combitips Plus 1 with the Repeater ® Plus 2 :The positions and dispensing steps that are marked in bold are based on Combitips Plus used with the original Repeater pipettePosition of the volume selection dial:0.5 1/1 1.5 2/2 2.5 3/3 3.5 4/4 4.5 5/5 5.5 6 6.5 7 7.5 8 8.5 9 9.5 10Maximum number of dispensing volumes:100 50/48 33 25/23 20 16/15 14 12/11 11 10/8 9 8 7 7 6 6 5 5 5 5Combitips Plus with the following volumes:0.1 mL 1 µL 2 µL 3 µL 4 µL 5 µL 6 µL 7 µL 8 µL 9 µL 10 µL 11 µL 12 µL 13 µL 14 µL 15 µL 16 µL 17 µL 18 µL 19 µL 20 µL0.2 mL 2 µL 4 µL 6 µL 8 µL 10 µL 12 µL 14 µL 16 µL 18 µL 20 µL 22 µL 24 µL 26 µL 28 µL 30 µL 32 µL 34 µL 36 µL 38 µL 40 µL0.5 mL 5 µL 10 µL 15 µL 20 µL 25 µL 30 µL 35 µL 40 µL 45 µL 50 µL 55 µL 60 µL 65 µL 70 µL 75 µL 80 µL 85 µL 90 µL 95 µL 100 µL1.0 mL 10 µL 20 µL 30 µL 40 µL 50 µL 60 µL 70 µL 80 µL 90 µL 100 µL 110 µL 120 µL 130 µL 140 µL 150 µL 160 µL 170 µL 180 µL 190 µL 200 µL2.5 mL 25 µL 50 µL 75 µL 100 µL 125 µL 150 µL 175 µL 200 µL 225 µL 250 µL 275 µL 300 µL 325 µL 350 µL 375 µL 400 µL 425 µL 450 µL 475 µL 500 µL5 mL 50 µL 100 µL 150 µL 200 µL 250 µL 300 µL 350 µL 400 µL 450 µL 500 µL 550 µL 600 µL 650 µL 700 µL 750 µL 800 µL 850 µL 900 µL 950 µL 1.0 mL10 mL 100 µL 200 µL 300 µL 400 µL 500 µL 600 µL 700 µL 800 µL 900 µL 1.0 mL 1.1 mL 1.2 mL 1.3 mL 1.4 mL 1.5 mL 1.6 mL 1.7 mL 1.8 mL 1.9 mL 2.0 mL25 mL 3 250 µL 500 µL 750 µL 1.0 mL 1.25 mL 1.5 mL 1.75 mL 2.0 mL 2.25 mL 2.5 mL 2.75 mL 3.0 mL 3.25 mL 3.5 mL 3.75 mL 4.0 mL 4.25 mL 4.5 mL 4.75 mL 5 mL50 mL 3 500 µL 1.0 mL 1.5 mL 2.0 mL 2.5 mL 3.0 mL 3.5 mL 4.0 mL 4.5 mL 5 mL 5.5 mL 6.0 mL 6.5 mL 7.0 mL 7.5 mL 8.0 mL 8.5 mL 9.0 mL 9.5 mL 10 mL1U.S. Pats. 5,620,660/5,620,6612U.S. Pats. 5,620,660/5,620,661/5,591/408/5,573,729325 mL and 50 mL Combitips Plus must be used with the supplied adapter.Ordering informationDescription Catalog No. List USD Catalog No. List USD Catalog No. List USDCombitips Plus tipsStandard(set of 100)Sterile(10 bags of10 tips = 100 tips)Eppendorf Biopur ®1(individually wrapped,set of 100)0.1 mL 022265954 $107.00 022267001 $134.00 022496000 $152.000.2 mL 022266004 107.00 022267108 134.00 022496026 152.000.5 mL 022266101 107.00 022267205 134.00 022496042 152.001 mL 022266209 107.00 022267302 134.00 022496069 152.002.5 mL 022266306 107.00 022267400 134.00 022496085 152.005 mL 022266403 107.00 022267507 134.00 022496107 152.0010 mL 022266501 107.00 022267604 134.00 022496123 152.0025 mL 022266551 132.00 022267655 157.00 022496131 174.0050 mL 022266608 132.00 022267701 157.00 022496140 174.0025 mL adapter (1 piece), blue 022266993 19.0050 mL adapter (1 piece), dark grey 022266705 19.0025 mL adapter (set of 7) 022496158 119.0050 mL adapter (set of 7) 022496166 119.00Combitips Plus tips assortment pack, includes five 022266624 86.00of each size plus one 50 mL and one 25 mL adapterCombitube (set of two), aspirating aid for removingliquids from volumetric flasks and tall bottles; CombitipsPlus tips sizes 5 mL, 25 mL and 50 mL022261550 54.001 Eppendorf Biopur Combitips (individaully wrapped) are guaranteed to be sterile,Rnase-, DNA-, ATP- and pyrogen-free.For pipette service and maintenance information, see pages100 and 101.Order from your laboratory supply dealer.Product appearance, specifications, and/or prices are subject to change without notice.

Manual and electronic dispensers71Mounting rack for Combitips Plus®Product featuresOrdering informationDescription Catalog No. List USDCombitips Rack, for mounting 022268007 $36.00Combitips Plus tips‡‡Autoclavable box with tray for storing up to 10 CombitipsPlus 2 tips from 0.1 mL to 10 mL sizes‡‡Particularly suitable for storing sterile products‡‡Can also be used as a stand for Repeater ® Plus 3 pipette‡‡Allows tips to be attached single-handedly‡‡Detachable sliding lid‡‡Nonslip stand‡‡Durable construction‡‡Labeling surfaceDescriptionTo save time and fully enjoy the improved convenience of theRepeater Plus and Repeater stream/Xstream, we have developedthe Combitips Rack—a box for 10 Combitips Plus tips that helpsyou to easily and single-handedly attach the tips.2U.S. Pats. 5,620,660/5,620,6613U.S. Pats. 5,620,660/5,620,661/5,591,408/5,573,7291 | Consumables | Pipetting | DispensingCombitips®For use only with original Repeater and Repeater Plus/8accessories. Combitips form a reliable and precise systemfor positive displacement dispensing in combination with theoriginal Repeater.Ordering informationDescription Catalog No. List USDCombitips, 0.05 mL set of 50, plus 96 x 10 µL pipette tips, autoclavable 022260952 $151.00Combitips, Standard, set of 1001.25 mL 022261100 127.0012.5 mL 022261401 127.00Combitips, Eppendorf Biopur ® , individually wrapped, set of 1001.25 mL 022495101 163.0012.5 mL 022495208 163.00In the U.S.: Eppendorf North America 800-645-3050 · In Canada: Eppendorf Canada Ltd. 800-263-8715E-mail: info@eppendorf.com · www.eppendorf.com

72 Manual and electronic dispensersDispensing acids, alkalis and organic solvents with the Repeater® Plus pipette1 | Info | Pipetting | DispensingWe have checked a number of acids, alkalis and organic solventswith a variety of viscosities and densities in our laboratories toprovide you with practical data in this regard.Vapor Density Viscosity100 µL 100 µL 1,000 µL 1,000 µL pressure at at 20 ºC at 20 ºCLiquid Conc. [d] [cv] [d] [cv] 20 ºC [hPa] [g/cm 3 ] [mPa s]Double-distilled water 100% ±0.6 ≤0.6 ±0.5 ≤0.25 23 1 1Acetone 100% 0.4 0.5 –0.2 0.3 240 0.79 0.32Acetonitrile 100% –0.4 1.1 –0.6 0.2 97 0.79 0.31 [25 ºC]Formid acid 98% –1.7 0.3 –1.9 0.2 42 1.22 1.8Ammonium hydroxide 32% 1.7 0.5 2.4 0.8 837 0.89 1.3 [26%]Aniline 100% –1.8 0.8 –1.3 0.3 0.5 1.03 4.4Dimethyl formamide (DMF) 100% 0.4 0.6 0.3 0.1 3.77 0.94 0.82Dimethyl sulfoxide (DMSO) 100% 0.1 0.7 0.1 0.2 0.56 1.1 2.14Ethyl acetate 100% –0.4 1.0 –0.4 0.2 97 0.9 0.44Ethanol 98% –0.1 0.6 0.1 0.1 58 0.78 1.20Formaldehyde 37% 0.8 0.3 0.8 0.1 1.3 1.08 2.5 [25 ºC]Isopropanol 99% –0.8 1.4 –0.3 0.2 43 0.78 2.2Perchloric acid 60% –2.0 0.5 –1.9 0.1 9 1.53 –Phosphoric acid 85% –1.7 0.9 –1.9 0.3 2 1.71 47Nitric acid 65% –1.3 0.3 1.9 0.1 9.4/9 1.41 1.49 [40%]Hydrochloric acid 37% –2.1 0.5 –1.7 0.3 213 1.19 2.0Sulfuric acid 95–97% –2.6 0.6 –4.4 0.3 0.0016 1.84 26.9Tetrachloromethane 100% –0.5 1.0 –0.2 0.2 122 1.59 0.96Tetrahydrofuran (THF) 100% –2.7 0.7 –0.3 0.2 173 0.88 0.48Toluene (methyl benzene) 100% –0.7 0.9 –1.1 0.1 29 0.87 0.6Trichloroethylene 100% –0.4 1.9 –0.1 0.3 77 1.46 0.55Trichloromethane 100% –0.1 0.4 0.3 0.2 213 1.47 0.56(chloroform)Triethylamine 100% –0.4 0.4 0.1 0.1 69 0.72 0.36Mean value 1.1 0.7 1.0 0.2Table 1: Random [d] and systematic [cv] measurement deviations in percent when dispensing selected liquidsThe experiments were performed using a Repeater Plus 1 with liquids at room temperature; the results of 10 dispensingoperations with a 5 mL Combitips Plus ®2 tip are shown.1U.S. Pats. 5,620,660/5,620,661/5,591,408/5,573,7292U.S. Pats. 5,620,660/5,620,661Order from your laboratory supply dealer.Product appearance, specifications, and/or prices are subject to change without notice.

Pipet fillers73Easypet® electronic pipet fillerProduct features‡‡Accurate, safe and fatigue-free series pipetting usinggraduated pipets from 0.1 mL to 100 mL‡‡Lightweight, balanced design provides comfortable,fatigue-free operation‡‡Touch-control adjustable speed for rapid or slowpipetting needs‡‡Fine control of the meniscus movement‡‡High aspirating speed: 25 mL in 5 seconds‡‡One-finger control for the dispensing andblow-out functions‡‡Cordless, works for up to 7 hours after recharging‡‡Autoclavable pipet adapters and filter holders‡‡Space-saving wall holder‡‡1-year warranty1 | Instruments | Pipetting | DispensingOrdering informationDescription Catalog No. List USDEasypet, with transformer, 022230204 $391.00wall holder and 2 membranefilters/0.45 µmMembrane filter, for Easypet,0.45 µm with black lettering,set of 5022232002 23.00Filter Disk, for Easypet, 0.45 µmwith blue lettering, set of 10022231006 56.00NiCd batteries KR 15/30 (qty. 3) 022236016 33.00Wall-mountable holder 022230905 27.00Pipet Helper® manual pipet fillerProduct features‡‡Safe and convenient filling and dispensing of glass andplastic pipets‡‡Easy-pull slide lever applies vacuum to eliminate the handstress caused by conventional “squeeze bulb” apparatus‡‡Single rocker-switch controls aspiration, dispensing andvacuum to save time‡‡Disposable, in-line filter prevents contamination‡‡Filters and pipet connectors can be autoclaved fordecontamination‡‡Easily disassembled for maintenance and cleaningOrdering informationPackaged in 100% recyclable material; for more informationon epGreen products, see pages 376 and 377.Description Catalog No. List USDPipet Helper, with filter disk 022230000 $76.00Filter Disk, 3 µm, pkg. of 10 022231103 50.00In the U.S.: Eppendorf North America 800-645-3050 · In Canada: Eppendorf Canada Ltd. 800-263-8715E-mail: info@eppendorf.com · www.eppendorf.com

74Bottle-top dispensers and buretsVarispenser® plus/Varispenser1 | Instruments | Pipetting | DispensingOrdering informationVarispenser: Bottletop dispenser, for external bottle threadsof 32 mm (sizes 1, 2 and 3) or 45 mm (sizes 4, 5 and 6),complete with telescopic tube, tool and 3 adapters (see below).Quality certificate. Varispenser plus: same as Varispenser,plus recirculation valve, valve toggle and media-specificfine adjustment.DescriptionOur Varispenser and Varispenser plus are ideal for dispensingaliquots of liquid from large supply bottles. Their smart designmake them capable of extracting reproducible dispensingvolumes with no reagent waste. Their tight seal provides the bestpossible protection against contact with caustic media, such assolvents, and they are chemically resistant and fully autoclavablefor the utmost in safety for you and your lab.Product features‡‡Extensive volume range (6 sizes)‡‡PFA-sealing of the slide piston prevents jamming‡‡Wiping piston design prevents crystallization of liquid‡‡Highly resistant to chemicals‡‡Rapid volume setting using precise graduation scale‡‡Easy disassembling and cleaning‡‡Telescopic filling tube for use with most bottles‡‡2-year warrantyOrdering informationSizeVolume rangeWith 3 adapters forouter diameters (mm)Dispensingsteps Inaccuracy 1 Imprecision 1 Catalog No. List USDVarispenser (adjustable-volume)1 0.5–2.5 mL 28, 40, 45 0.05 mL ±0.6% ≤0.1% 4960000019 $432.002 1–5 mL 28, 40, 45 0.1 mL ±0.5% ≤0.1% 4960000027 432.003 2–10 mL 28, 40, 45 0.2 mL ±0.5% ≤0.1% 4960000035 432.004 5–25 mL 32, 38, 40 0.5 mL ±0.5% ≤0.1% 4960000043 580.005 10–50 mL 32, 38, 40 1 mL ±0.5% ≤0.1% 4960000051 614.006 20–100 mL 32, 38, 40 2 mL ±0.5% ≤0.1% 4960000060 909.00Varispenser plus (adjustable-volume)1 0.5–2.5 mL 28, 40, 45 0.05 mL ±0.6% ≤0.1% 4961000012 456.002 1–5 mL 28, 40, 45 0.1 mL ±0.5% ≤0.1% 4961000020 456.003 2–10 mL 28, 40, 45 0.2 mL ±0.5% ≤0.1% 4961000039 456.004 5–25 mL 32, 38, 40 0.5 mL ±0.5% ≤0.1% 4961000047 629.005 10–50 mL 32, 38, 40 1 mL ±0.5% ≤0.1% 4961000055 634.006 20–100 mL 32, 38, 40 2 mL ±0.5% ≤0.1% 4961000063 978.001The data for imprecision (random measurement error) and inaccuracy (systematic measurement error) are given according to EN ISO 8655.Bottle-top dispenser is not a substitution for a bottle closure. Volatile liquids can disappear.For more information go to www.eppendorfna.com/varispenserOrder from your laboratory supply dealer.Product appearance, specifications, and/or prices are subject to change without notice.

Bottle-top dispensers and burets75Top Buret1 | Instruments | Pipetting | DispensingProduct features‡‡Patented recirculation valve for priming withoutloss of reagent‡‡Voltage supply with long-life primary cells andcharging display‡‡Dispensing range from 0.01 mL to 999.9 mL‡‡Removable electronics unit‡‡Modular, service-friendly construction‡‡Simple calibration program‡‡Discharge tube can be rotated a full 360°‡‡Adjustable filling tube horizontal: 142–220 mm;vertical: 10–200 mm‡‡Telescopic filling tube for automatically settingthe length from 210 mm to 370 mmDescriptionOur innovative Top Buret sets new standards for manualtitration. Its pulse-free dispensing technique allows the desiredvolumes to be dispensed continuously, thus making titrationsimpler and safer, with precision values well within requiredlimits. The volume is dispensed by simply rotating the wheels:Model M features a dispensing rate of 2,500 µL per rotation;Model H features a rate of 5,000 µL per rotation. The displayreadings on both models range from 0.01 mL to 999.9 mL.This bottle-top buret is extremely sturdy, and its user-friendlydisplay and control features provide simple, fatigue-freeoperation.Application‡‡2-year warranty ‡‡Continuous, pulse-free dispensing technique for rapid titrationOrdering informationDescription Inaccuracy 1 Imprecision 1 Catalog No. List USDBottle-top buret with recirculation valve, valve toggle, telescopic filling tube, adjustable discharge tube, 3 adapters for40/38/32 mm bottle threads, 2 x 1.5 V microbatteries. Quality certificate.Top Buret M, 2.5 mL per rotation ±0.2% ≤0.1% 4965000017 $1,029.00Top Buret H, 5 mL per rotation ±0.2% ≤0.1% 4965000025 1,029.00Dry tube 4960851000 69.001The data for imprecision (random measurement error) and inaccuracy (systematic measurement error) are given according to EN ISO 8655.Bottle-top dispenser is not a substitution for a bottle closure. Volatile liquids can disappear.In the U.S.: Eppendorf North America 800-645-3050 · In Canada: Eppendorf Canada Ltd. 800-263-8715E-mail: info@eppendorf.com · www.eppendorf.com

76Automated pipettingepMotion® automated pipetting system1 | Instruments | Pipetting | DispensingEasy-to-use‡‡Intuitive software design makes learning easy‡‡3D simulation software for checking methods‡‡Patented optical sensor examines the worktable forcorrect loading‡‡Pre-defined software files for all manufacturer’s labwareDescriptionepMotion automates all of your pipetting tasks, delivering themost accurate pipetting and relief from tedious bench work foreveryone, everyday. Whether it’s a sample or reagent transfer,assay setup or nucleic acid extraction, even with the smallestsample numbers any application will benefit from automation.Pipetting is more accurate, reproducible and completelystandardized.Accurate‡‡Superior automated liquid handling– Single- and eight-channel pipetting tools– Pipetting range from 1 to 1,000 µL, alwaysfrom air to minimize cross-contamination– Typical pipetting precision below 2% CV at 1 µL*Automated pipetting is totally hands-free, creating a moreefficient and ergonomic workplace. With six models, twoinstrument sizes and multiple pipetting deck combinationsto choose from, epMotion is the perfect companion for yourworkload. Transition from manual protocols is simple withpredefined protocols, and the powerful labware databaseensures that virtually any tube or plate can be used.Flexible‡‡Compatible with plates up to 384 wells and tubesup to 50 mL‡‡Modular design with multiple add-on’s allows forgrowth with the laboratory‡‡Easily automates most routine and complex liquidhandling tasks* Eppendorf application note 168: “Measuring the Accuracy and Precision of theepMotion 5070 Workstation Using the Artel Multichannel Verification System (MVS ® ).”Order direct from Eppendorf North America.Product appearance, specifications, and/or prices are subject to change without notice.

Automated pipetting77epMotion® applicationsepMotion Capacity Applications5070 4-position deck and3 virtual positionsSystem description page 785070 CB 4-position deck and3 virtual positions,specially designed tofit into a laminar flowhood or fume hoodSystem description page 80General pipetting tasks‡ Serial dilutions‡ Tube to plate transfer‡ Reformatting of plates‡ Concentration normalization‡ Hit-picking‡ Reagent transfer‡ PoolingExamples: All routine pipetting, e.g. sample/reagent transfer, qPCR or immunoassaysCell culture‡ Cell separation‡ Change of media‡ Cytotoxicity assays‡ Growth curves‡ Soft agar assays‡ Cell-based assays‡ Apoptosis assaysExamples: Cell culture maintenanceand assays1 | Instruments | Pipetting | Dispensing5075 LH 12-position deck‡ Transporting‡ Stacking ability‡ 3 thermo modules(optional)Genomics applications‡ Real-time PCR set-up‡ Sequencing set-up‡ SNP detection‡ ForensicsExamples: All routine pipetting, e.g. sample/reagent transfer, qPCR or immunoassaysSystem description page 825075 VAC 11-position deck and1 vacuum stationSystem description page 845075 MC 9-position deckand 1 positionfor Mastercycler ® epNucleic acid purification‡ gDNA from various sample types‡ Total and viral RNA‡ Plasmid preparation‡ BAC purification‡ PCR and sequencing clean-up‡ Bacterial DNAExamples: Nucleic acid extraction, Plug‘n’Prep ®protocols, filtration, and solid phase extractionComplex assays‡ Luminex ® assay set-up‡ HLA genotyping‡ PCR set-up with automated amplificationExamples: Fully automated PCR: set-upand amplificationSystem description page 885075 TMX 11-position deck and1 TMX moduleSystem description page 86Typical applications‡ Immunoassays‡ Magnetic bead purification‡ Hit picking‡ Cell assays‡ Nucleic acid preparationExamples: Compound screening, PCR set-up,concentration normalizationsIn the U.S.: Eppendorf North America 800-645-3050 · In Canada: Eppendorf Canada Ltd. 800-263-8715E-mail: info@eppendorf.com · www.eppendorf.com

78 Automated pipettingepMotion® 50701 | Instruments | Pipetting | DispensingSafety doorepMotion is fully enclosed.Operation is paused when the dooris opened during operation.Optical sensorChecks loading of the worktable for tips(type and number), labware type, andliquid volumes in vessels.4-position worktableWith 3 virtual positionsfor increased throughputPipetting toolsHigh-precision pipetting tools based onthe Eppendorf ® air cushion liquid handlingtechnology. Unique free-jet dispensingminimizes the risk of cross-contamination.LabwareCompatible with plates up to 384-wellsand tubes from 0.2 mL to 50 mLSmall footprintOnly 65 cm × 48 cm. Fits on eventhe smallest available lab bench.Order direct from Eppendorf North America.Product appearance, specifications, and/or prices are subject to change without notice.

Automated pipetting79epMotion® 5070Product features‡‡4 labware positions, plus three virtual positions‡‡Pipetting range from 1 µL to 1,000 µL‡‡Compatible with micro- and PCR plates‡‡Compatible with plates up to 384 wells‡‡Compatible with tubes from 0.2 mL to 50 mL‡‡Liquid level detection without conductive tips‡‡Optical sensor* checks the loaded deck‡‡Safety hood with monitored front door‡‡Easily exchangeable pipetting tool* U.S. Pat. 6,819,437Applications‡‡Serial dilutions‡‡Reagent distribution‡‡Reformatting into plates‡‡Sample transfer from tubes to plates‡‡Sample normalization‡‡Media change for cell culture‡‡Real-time PCR set-up‡‡Cell-based assay set-up‡‡Immunoassay set-upDescriptionepMotion 5070 is the most compact solution for accurateand reproducible automated pipetting. It is a perfect matchfor all routine applications, such as serial dilutions, reagentdistribution, sample transfer from tubes to plates andsample normalization.1 | Instruments | Pipetting | DispensingDue to its excellent pipetting accuracy, epMotion is therecommended solution for real-time PCR—enabling setupswith less reagent volume and excellent reproducibility.Its pipetting technology is based on the classic Eppendorf ®piston-stroke pipettes; thus, protocols previously carriedout manually are easily transferred to epMotion 5070. Itscompatibility to a wide range of predefined consumables alsoallows established procedures to be maintained. Before everyrun the optical sensor checks that the required labware iscorrectly positioned on the deck, the liquid volumes in vesselsand it also checks tip type and quantity available.30 mL and 100 mL reagent reservoirsThe reagent reservoirs are “PCR clean” as well asautoclavable, and they can be easily placed in thereagent rack; their special geometry minimizes residualvolume and makes them ideal vessels for reagents.3D simulation software3D simulation software provided as a standard feature.Thermorack for Safe-Lock tubesRack for up to 24 micro test tubes in the formats0.5/1.5/2.0 mL.For epMotion ordering information, see page 96.For more epMotion accessories, see page 90.In the U.S.: Eppendorf North America 800-645-3050 · In Canada: Eppendorf Canada Ltd. 800-263-8715E-mail: info@eppendorf.com · www.eppendorf.com

80 Automated pipettingepMotion® 5070 CB1 | Instruments | Pipetting | DispensingLight barrier beamMonitors the closureof the bench hood.Optical sensorChecks loading of the worktable for tips (type and number),labware type, and even liquid volumes in vessels.4-position worktableSpecially designed for cell cultureand cell based assays.Compact designOnly 65 cm × 48 cm × 63 cm,(W × D × H) fits most laminar flow hoods.Order direct from Eppendorf North America.Product appearance, specifications, and/or prices are subject to change without notice.

Automated pipetting81epMotion® 5070 CBProduct features‡‡Compatible with almost all culture hoods‡‡Innovative light barrier safety lock technology‡‡Preprogrammed cell culture applications‡‡Sterile pipette tips‡‡Autoclavable pipetting tools and accessoriesApplications‡‡Cell culture maintenance‡‡Cell aliquoting‡‡Media exchange‡‡Apoptosis testing‡‡Cyto-toxicity testing‡‡Cell viability testing‡‡Soft agar testsDescriptionThe epMotion 5070 CB is the first automated pipetting systemfor cell culture applications allowing all of the benefits ofautomated pipetting to be available for liquid handling tasksinside a laminar flow or fume hood. Cell maintenance is oneof the most repetitive tasks in life science research. TheepMotion 5070 CB allows you to automate key processes suchas seeding cells, media changes, or performing cytotoxicitytests. It enables you to handle 6-, 24- or 48-well plates or evendenser formats from 96 to 384 wells. Almost all single tube sizessuch as cryotubes ® , Safe-Lock and blue cap tubes up to 50 mLare supported.1 | Instruments | Pipetting | DispensingepMotion 5070 CBUsed on a sterile workbench.Thermorack for cryotubesPre-cooled rack for up to 24 cryotubes.For epMotion ordering information, see page 96.For more epMotion accessories, see page 90.In the U.S.: Eppendorf North America 800-645-3050 · In Canada: Eppendorf Canada Ltd. 800-263-8715E-mail: info@eppendorf.com · www.eppendorf.com

82 Automated pipettingepMotion® 5075 LH1 | Instruments | Pipetting | DispensingGripperMoves labware to different positionson the deck.Automated tool exchangeEnables the execution of morecomplex pipetting patterns.3 thermal element optionsPeltier-based thermal element for heatingor cooling of samples or reagents from 0–110 °C.12-position worktableFor applications requiring higher throughput.epMotion PCIntuitive software guides you throughyour everyday pipetting tasks.Plate stackingUp to five microplates or two deepwell platescan be stacked on each other by the gripper.Order direct from Eppendorf North America.Product appearance, specifications, and/or prices are subject to change without notice.

Automated pipetting83epMotion® 5075 LHProduct features‡‡12 labware positions‡‡Automatic exchange of all tools‡‡Pipetting range from 1 µL to 1,000 µL‡‡Thermal module option‡‡Plate gripper option‡‡Compatible with tubes from 0.2 mL to 50 mL‡‡Compatible with plates up to 384 wells‡‡Plate stacking‡‡Optical sensor* checks the loaded deck‡‡Detection of tip size and tip quantity‡‡Safety hood with monitored front door‡‡Modular system: Upgrade to 5075 VAC, 5075 TMXand 5075 MC‡‡Liquid level detection with unique optical sensor* U.S. Pat. 6,819,437Applications‡‡Serial dilutions‡‡Reagent distribution‡‡Reformatting into plates‡‡Sample transfer from tubes to plates‡‡Sample normalization‡‡Pooling of samples‡‡Media change for cell culture‡‡Real-time PCR set-up‡‡Immuno- and cell-based assay set-up‡‡Compound testing‡‡Magnetic bead applicationsDescriptionepMotion 5075 LH is the ideal solution for advanced liquidhandling procedures. It offers the same outstanding accuracyand precision as the epMotion 5070, making it an ideal tool fordemanding small-volume applications, such as real-time PCRsetup, magnetic bead purification and all routine pipetting tasks.The 12-position deck and automatic tool exchange expand theapplication range to include complex pipetting patterns andhigher sample numbers. With heating and cooling options aswell as the availability of a gripper for labware transport on thedeck, the epMotion 5075 LH is a versatile and flexible automatedpipetting system that is able to meet the needs of the mostdemanding research.1 | Instruments | Pipetting | DispensingReservoir rack with tube modulesIndividually configured and temperature controlled.Thermal ModulesUp to three can be used for active heating and cooling ofThermal Racks, Thermal Blocks and Reagent Reservoir Modules.For epMotion ordering information, see page 96.For more epMotion accessories, see page 90.In the U.S.: Eppendorf North America 800-645-3050 · In Canada: Eppendorf Canada Ltd. 800-263-8715E-mail: info@eppendorf.com · www.eppendorf.com

84 Automated pipettingepMotion® 5075 VAC1 | Instruments | Pipetting | DispensingGripperMoves plates and assembles/disassemblesthe vacuum station without manual intervention.Integrated vacuum pumpNo tubing, wiring, and reservoirs to maintain.Silent operation.2 thermal element optionsPeltier-based thermal element forheating or cooling of samples or reagents.TMX thermal element optionFor shaking, heating and coolingof samples and reagents.11-position worktableDesigned to automate vacuum-based extractionsand downstream applications for up to 96 samples.Vacuum stationFully integrated.Adapts automatically to any filter plate type.Order direct from Eppendorf North America.Product appearance, specifications, and/or prices are subject to change without notice.

Automated pipetting85epMotion® 5075 VACProduct featuresSame as epMotion 5075 LH plus:‡‡11 labware positions‡‡One vacuum position‡‡Integrated vacuum pump‡‡Vacuum is fully software controlled‡‡Universal spring loaded vacuum frame‡‡Plug‘n’Prep ® validated protocolsApplicationsSame as epMotion 5075 LH plus:‡‡Vacuum based purification applications‡‡Solid phase extraction‡‡Filtration‡‡Predefined and tested methods for:– Plasmid and BAC purification– gDNA from blood and tissue– gDNA from plants– Sequencing and PCR clean-up– Total RNADescriptionComplex applications such as nucleic acid purification shouldnot tie lab personnel to the workbench. The epMotion 5075 VACallows true walk-away automation of purification protocols thatrely on vacuum filtration.Pre-validated protocols are available for most standardapplications, such as plasmid DNA, genomic DNA and RNApurification. The protocols are predefined on the systemand setup takes only a few minutes. Additional customizedapplications can be developed easily using epMotion epBluePC software.The unique Eppendorf ® silicon mat helps to save time andmoney by allowing you to use only portions of a 96-well platewhen working with smaller sample numbers. The VAC thermolid also reduces the extraction time substantially by acceleratingthe ethanol evaporation step.1 | Instruments | Pipetting | DispensingIntegrated vacuum stationCompletely integrated vacuum chamber and pump that adaptsto any filter plate.Labware handlingReconfiguration of the vacuum chamber and the transport oflabware takes place automatically with the gripper.For epMotion ordering information, see page 96.For more epMotion accessories, see page 90.In the U.S.: Eppendorf North America 800-645-3050 · In Canada: Eppendorf Canada Ltd. 800-263-8715E-mail: info@eppendorf.com · www.eppendorf.com

86 Automated pipettingepMotion® 5075 TMX1 | Instruments | Pipetting | DispensingAutomated tool exchangeEnables the execution of morecomplex pipetting patterns.GripperMoves plates onto the TMX positionwithout manual intervention.11-position worktableDesigned to automate vacuum-based extraction anddownstream applications for up to 96 samples.Integrated TMX moduleUnique 2-D mixing control improves yieldsand applications results.Vacuum station optionFor vacuum basedextraction procedures.TMX thermal elementPeltier-based thermal element forheating or cooling of samples or reagents.Order direct from Eppendorf North America.Product appearance, specifications, and/or prices are subject to change without notice.

Automated pipetting87epMotion® 5075 TMXProduct featuresSame as epMotion 5075 LH plus:‡‡11 labware positions‡‡TMX module—active mixing/vortexing andtemperature control‡‡Temperature range: 4 °C to 95 °C‡‡Automatic lock-in of the labware on the TMX position‡‡Pipetting in other deck positions is possible duringthe mixing stepsApplicationsSame as epMotion 5075 LH plus:‡‡Integrated shaker/vortexer with heating and cooling‡‡MagBead based extractions‡‡Pre-programmed methods for gDNA and RNA‡‡gDNA from blood and fabric‡‡Sequencing PCR Clean up from plants‡‡Total RNA extractions‡‡Unique 2D Mix-Control for optimal resuspension‡‡DNA/RNA library sample preparationDescriptionThe epMotion 5075 TMX is the most flexible member of theepMotion family of automated pipetting systems. It includesan integrated TMX module to shake, heat or cool samples andreagents. The TMX module can be loaded and unloaded withthe gripper and pipetting to the mixer is possible before andafter mixing steps. The TMX module is fully software controlledenabling pipetting onto other worktable positions while themixer is in operation.1 | Instruments | Pipetting | DispensingTMX mixing is based on Eppendorf’s 2D Mix-Control technology.Orbital shaking combined with the perfect balance of speedand mixing radius assures the best stirring results, for examplein resuspending bacteria pellets or magnetic beads. The2DMix-Control eliminates the need for additional centrifugationsteps as the orbital shaking prevents droplets from collectingon the sides of the tube or plate wells.Plate handling capabilitiesA plate gripper allows for the transport of labware to and fromthe TMX module.Integrated TMX moduleEnables the mixing, heating and cooling of samples and reagents.For epMotion ordering information, see page 96.For more epMotion accessories, see page 90.In the U.S.: Eppendorf North America 800-645-3050 · In Canada: Eppendorf Canada Ltd. 800-263-8715E-mail: info@eppendorf.com · www.eppendorf.com

88 Automated pipettingepMotion® 5075 MC1 | Instruments | Pipetting | DispensingAutomated sealingSealing of plates with reusablesilicon-based cycle lock system.GripperMoves the plateto the Mastercycler ® ep.Integrated Mastercycler ep96- or 384-wellblock options.Order direct from Eppendorf North America.Product appearance, specifications, and/or prices are subject to change without notice.

Automated pipetting89epMotion® 5075 MCProduct featuresSame as epMotion 5075 LH plus:‡‡9 labware positions‡‡Integrated Mastercycler ® ep gradient* with motorized lid‡‡96-or 384-well PCR block options‡‡Robust, high-quality aluminum or silver block‡‡Integrated Mastercycler ep software‡‡Unique cycle-lock silicon sealing system‡‡Simultaneous PCR reaction and liquid handling possible* Mastercycler ep gradient sold separatelyPractice of the patented polymerase chain reaction (PCR) process requires a license.The Mastercycler is an Authorized Thermal Cycler and may be used with PCR licensesavailable from Applied Biosystems. Its use with Authorized Reagents also provides alimited PCR license in accordance with the label rights accompanying such reagents.ApplicationsSame as epMotion 5075 LH plus:‡‡Fully automated sample preparation and subsequentamplification in the Mastercycler ep‡‡Automated PCR in 96-/384-well plate formats‡‡Automated sequencing reactions in 96-/384-wellplate formats‡‡In vitro plasmid amplification‡‡Automated DNA extraction and profiling of forensicFTA ® samplesDescriptionepMotion 5075 MC combines sample preparation and PCR inan enclosed system for automated “walk-away” amplificationreactions. This version can be equipped with all motorizedlid models of the Eppendorf ® Mastercycler ep line ofthermal cyclers.1 | Instruments | Pipetting | DispensingThe cycler software is integrated in the epMotion software, soboth system components can easily be programmed with thesame innovative controls. Deck positions can be used for liquidhandling protocols while the cycler is running.Ready to goThe epMotion 5075 MC and Mastercycler ep provide a greatcombination for the automation and execution of PCR reactions.Dispensing without cross-contaminationAmplification of the 535 bp fragment of the human b-globin genein a 384-well format, where every other well contains a negativecontrol in a checkerboard pattern.For epMotion ordering information, see page 96.For more epMotion accessories, see page 90.In the U.S.: Eppendorf North America 800-645-3050 · In Canada: Eppendorf Canada Ltd. 800-263-8715E-mail: info@eppendorf.com · www.eppendorf.com

90Automated pipettingepMotion® automated pipetting system accessories1 | Instruments | Pipetting | DispensingReservoir rackEnables the use of up to 7reagent rack modules or reservoirs.Thermoracks and adaptersFor use with 96- and 384-wellPCR plates on thermal elements.Reservoir rack modules TCModules for the reservoir rack.Seven module sizes are availableto use tubes from 0.2 mL to 50 mL.Temperature-controlled when usedwith a thermal module.Height adapterVarious height adapters enableexact height level adjustmentand accelerated processingof microplates.30 mL and 100 mL reagent reservoirThe reagent reservoirs are both“PCR clean,” autoclavable, andcan be placed in the reservoir rack.Their special geometry minimizesresidual volume.Thermoblock for PCR platesThermal block holds 96-or 384-well plates and can betransported by gripper. Allowsfor temperature control whenused with a thermal module.Racks for single test tubesFor micro test tubes, glass,or plastic tubes of various sizes.Gripper for epMotion 5075For the transport of labwareon the worktable and for automaticoperation of the vacuum manifold.Thermoadapter DWP 96For heating or cooling ofdeepwell plates. Plates canbe exchanged by the gripper.Thermal module(only epMotion 5075)For thermoracks and 96- and 384-wellPCR plates. Cool or heat up to3 positions on the deck.Thermoracks for 24 ×Safe-Lock 0.5/1.5/2 mL tubesFor 24 micro test tubes.Dispensing tools3 different dispensing tools areavailable from either 1–50 µL,20–300 µL or 40–1,000 µL in eithersingle- or 8-channel formats.Order direct from Eppendorf North America.Product appearance, specifications, and/or prices are subject to change without notice.For ordering information,see pages 97 to 99.

Automated pipetting91epMotion® tools and tipsAccuracy and reproducibility deliveredBased on Eppendorf ® classic air cushion pipetting technology‡‡No tubes, no wires, no air bubbles‡‡As simple to operate and maintain as a manual pipette‡‡Excellent pipetting precision, typically below 2% CV at 1 µL*‡‡Automatic alert when pipetting tools need calibration‡‡Ultra-low dead volume pipetting‡‡Every tool tested and certified to ISO 85540* Eppendorf application note 168: “Measuring the Accuracyand Precision of the epMotion ® 5070 Workstationusing the Artel Multichannel Verification System (MVS ® ).”1 | Consumables | Pipetting | DispensingepT.I.P.S. ® MotionSpecially selected tips for use in automated systems. Each tipis inspected for straightness prior to packaging. This ensuresa perfect handling of 96- and 384-well micro and PCR plates.‡‡Box packaging ensures contamination-free tip loading‡‡Non-carbonized, pure virgin polypropylene, both tip andbox are recyclable‡‡Standard, filter, and sterile tips available‡‡PCR-clean quality grade for filter tips‡‡50 µL filter tip‡‡50 µL tip without filter‡‡300 µL filter tip‡‡300 µL tip without filter‡‡1,000 µL filter tip‡‡1,000 µL tip without filterIn the U.S.: Eppendorf North America 800-645-3050 · In Canada: Eppendorf Canada Ltd. 800-263-8715E-mail: info@eppendorf.com · www.eppendorf.com

92Automated pipettingepMotion® Control: epMotion PC version1 | Instruments | Pipetting | DispensingProduct features‡‡The network interface of the integrated PC allowsthe remote control of the epMotion via direct ethernetconnection. Method files can be shared using thecomputer network or USB port.‡‡The epMotion PC version defines a new dimensionin ease-of-use and safety. It’s the first to include anIndustrial PC which ensures long term reliability throughstandardization and the multiple quality checks of eachindividual PC that takes place.epMotion Control: control panelProduct features‡‡Graphical user interface‡‡Drag and drop programming‡‡Windows ® explorer style organization‡‡User management‡‡Run reports‡‡Software preinstalled on the control panelepMotion Control: editor version—easy off-line program managementepMotion editorA separate, off-line software package for generating andediting methods as well as printing and archiving programprocesses on your PC.Product features‡‡Programming functions analogous to the control panel‡‡Graphical user interface identical to epBlue‡‡Print function‡‡Archiving function‡‡Transfer of methods using the MMC cardOrder direct from Eppendorf North America.Product appearance, specifications, and/or prices are subject to change without notice.

Automated pipetting 93epMotion® PC software: epBlue—intuitive operation and programmingDescriptionSimple and clear, epBlue guides you through your everydaypipetting tasks: Choose your objective in the home sectionand follow the epBlue commands from left to right with uniqueguiding menus and the consistent, tab-based structure. Withthe unmatched ease-of-use of epBlue, even complex methodscan be generated in minutes.Pipetting pattern recognition, labware database, and liquidclasses are built-in and organized for intuitive use. Simply set upyour worktable, select the pipetting commands, simulate, andlaunch. Operating and programming epBlue is fast and easy.1 | Software | Pipetting | Dispensing‡‡The home screen provides fast access to the mainfunctions and personal list of the recently used methods,while the intuitive graphical user interface providesa consistent, tab-based workflow from left to right‡‡A consistant tab-based structure guides the userthrough creating a methodepBlue ID —epMotion PC softwareDescriptionepBlue ID is a software module for epMotion PC versionsthat allows the epMotion to be employed in application areasrequiring process documentation.epBlue ID allows for safe data exchange with laboratoryinformation management systems (LIMS) and simplifies externalcommunication. The barcode scanner enables the user to recordbarcodes on every container type, including a wide range oftube types and microplates. Reagents can be documented withtype and lot number. After scanning the barcodes, epBlue IDwill store the scanning data in its database from where datacan be rechecked at any time. A result file containing sampleID’s and their final location is generated by epBlue ID when theliquid handling process is completed. The result file can then beexported to a network drive and uploaded from a LIMS.Receipt/registration ofdelivered samplesStorageLIMS/ELNCustomer databaseWorklistResultfilesCSVXMLepBlue IDCSVXMLScanning oflabeled vesselsBarcodeReadingSampleProcessingProtocol/DocumentationIn the U.S.: Eppendorf North America 800-645-3050 · In Canada: Eppendorf Canada Ltd. 800-263-8715E-mail: info@eppendorf.com · www.eppendorf.com

94Automated pipettingepMotion® automated pipetting system1 | Instruments | Pipetting | DispensingTechnical specifications 5070 5075 LH 5075 VACSet Eppendorf Quality epMotion® 5070 5075 LH epMotion® 5075 VACSurfaceSystem: Width 39 in/98 cm 56 in/140 cm 56 in/140 cmDepth 30 in/75 cm 30 in/75 cm 30 in/75 cmDimensions (W x D x H)Device: Width 26 in/65 cm 43 in/107 cm 43 in/107 cmDepth 19 in/48 cm 24 in/61 cm 24 in/61 cmHeight 25 in/63 cm 27 in/67 cm 27 in/67 cmControl panel: Width 10 in/25 cm 10 in/25 cm 10 in/25 cmDepth 6 in/15 cm 6 in/15 cm 6 in/15 cmHeight 4 in/11 cm 4 in/11 cm 4 in/11 cmWeightDevice: 99 lb/45 kg 187 lb/85 kg 198 lb/90 kgControl panel: 3 lb/1.2 kg 3 lb/1.2 kg 3 lb/1.2 kgPower supplyVoltage: 110–130 V ±10% 110–130 V ±10% 110–130 V ±10%Frequency: 50–60 Hz ±5 % 50–60 Hz ±5% 50–60 Hz ±5%Max. output: 80 W 1,000 W 1,000 WDispensing toolsVolume: 1 µLSystematic measurement error: ±15% ±15% ±15%Random measurement error: ≤5% ≤5% ≤5%Volume: 50 µLSystematic measurement error: ±1.2% ±1.2% ±1.2%Random measurement error: ≤0.4% ≤0.4% ≤0.4%Volume: 1,000 µLSystematic measurement error: ±0.7% ±0.7% ±0.7%Random measurement error: ≤0.15% ≤0.15% ≤0.15%in pipetting mode, free jet, without pre-wetting, with distilled water, at 20 °CConductorX, Y, Z Positioning:Systematic measurement error: ±0.3 mm ±0.3 mm ±0.3 mmRandom measurement error: ±0.1 mm ±0.1 mm ±0.1 mmPositions in MTP-Format: 4 12 12DetectorOptical confocal infrared detector:contact-free detection of liquid levels, tools used, labware worktables,tip types and quantitiesOptical sensor:*liquid surface must be 90 ±3° to the vertical plane of the optical sensorGripperCarrying capacity: ≤1,200 g ≤1,200 g ≤1,200 gVacuum unitMax. output: – – 35 L/minSuction range: – – 0.1–0.85 ±0.05 PaSuction time: – – 1–99 min* U.S. Pat. 6,819,437Order direct from Eppendorf North America.Product appearance, specifications, and/or prices are subject to change without notice.

Automated pipetting95epMotion® automated pipetting systemTechnical specifications 5075 TMX 5075 MC 5070 CBSet Eppendorf Quality epMotion® 5075 LH epMotion® 5075 MCSurfaceSystem: Width 56 in/140 cm 56 in/140 cm 39 in/98 cmDepth 30 in/75 cm 30 in/75 cm 24 in/62 cmDimensions (W x D x H)Device: Width 43 in/107 cm 43 in/107 cm 26 in/65 cmDepth 24 in/61 cm 24 in/61 cm 19 in/48 cmHeight 27 in/67 cm 27 in/67 cm 25 in/63 cmControl panel: Width 10 in/25 cm 10 in/25 cm 10 in/25 cmDepth 6 in/15 cm 6 in/15 cm 6 in/15 cmHeight 4 in/11 cm 4 in/11 cm 4 in/11 cmWeightDevice: 209 lb/95 kg 224 lb/102 kg 99 lb/45 kgControl panel: 3 lb/1.2 kg 3 lb/1.2 kg 3 lb/1.2 kgPower supplyVoltage: 110–130 V ±10% 110–130 V ±10% 110–130 V ±10%Frequency: 50–60 Hz ±5% 50–60 Hz ±5 % 50–60 Hz ±5%Max. output: 1,000 W 1,000 W 70 WDispensing toolsVolume: 1 µLSystematic measurement error: ±15% ±15% ±15%Random measurement error: ≤5% ≤5% ≤5%Volume: 50 µLSystematic measurement error: ±1.2% ±1.2% ±1.2%Random measurement error: ≤0.4% ≤0.4% ≤0.4%Volume: 1,000 µLSystematic measurement error: ±0.7% ±0.7% ±0.7%Random measurement error: ≤0.15% ≤0.15% ≤0.15%in pipetting mode, free jet, without pre-wetting, with distilled water, at 20 °CConductorX, Y, Z Positioning:Systematic measurement error: ±0.3 mm ±0.3 mm ±0.3 mmRandom measurement error: ±0.1 mm ±0.1 mm ±0.1 mmPositions in MTP-Format: 10 10 4DetectorOptical confocal infrared detector:contact-free detection of liquid levels, tools used, labware worktables,tip types and quantitiesOptical sensor:*liquid surface must be 90° ±3° to the vertical plane of the optical sensorGripperCarrying capacity: ≤1,200 g ≤1,200 g ≤1,200 gVacuum unitMax. output: – – –Suction range: – – –Suction range: – – –1 | Instruments | Pipetting | Dispensing* U.S. Pat. 6,819,437In the U.S.: Eppendorf North America 800-645-3050 · In Canada: Eppendorf Canada Ltd. 800-263-8715E-mail: info@eppendorf.com · www.eppendorf.com

96Automated pipettingepMotion® automated pipetting system1 | Instruments | Pipetting | DispensingOrdering informationDescription Catalog No. List USDepMotion automated pipetting systemsepMotion 5070 with control panel, basic device incl. control panel, mouse, software, waste box, 960000005 InquireMMC, and reader, 50/60 Hz, 100–240 VepMotion 5070 CB with control panel, epMotion to be operated inside closed containers, basic 960000021 Inquiredevice incl. control panel, mouse, software, waste box, MMC, and reader, 50/60 Hz, 100–240 VepMotion 5075 LH with control panel, basic device incl. control panel, software, waste box,960020006 InquireMMC, and reader, 50/60 Hz, 100–240 VepMotion 5075 VAC with control panel, basic device incl. control panel, software, vacuum system, 960020014 Inquiregripper, waste box, MMC, and reader, 50/60 Hz, 100–240 VepMotion 5075 MC with control panel, basic device incl. control panel, software, cycler bay,960020022 Inquiregripper, waste box, MMC, and reader, 50/60 Hz, 100–240 V. Mastercycler ® not included.epMotion 5075 TMX with control panel, basic device incl. Thermomixer ® , control panel, mouse, 960020033 Inquiresoftware, waste box, MMC and reader, 50/60 Hz, 100–240 VepMotion with integrated PCepMotion 5070 PC version960000111 InquireSame as 5070 catalog no. 960000005 but with integrated industrial PC, keyboard, and mouseinstead of control panel. Includes monitor and epBlue software.epMotion 5070 CB PC version960000200 InquireSame as 5070 catalog no. 960000021 but with integrated industrial PC, keyboard, and mouseinstead of control panel. Software and monitor not included.epMotion 5075 LH PC version960020111 InquireSame as 5075 catalog no. 960020006 but with integrated industrial PC, keyboard, and mouseinstead of control panel. Includes monitor and epBlue software.epMotion 5075 VAC PC version960020222 InquireSame as 5075 catalog no. 960020014 but with integrated industrial PC, keyboard, and mouseinstead of control panel. Includes monitor and epBlue software.epMotion 5075 MC PC version960020333 InquireSame as 5075 catalog no. 960020022 but with integrated industrial PC, keyboard, and mouseinstead of control panel. Includes monitor and epBlue software.epMotion 5075 TMX PC version960020444 InquireSame as 5075 catalog no. 960020033 but with integrated industrial PC, keyboard, and mouseinstead of control panel. Includes monitor and epBlue software.Monitor, 22" TFT monitor to be used with epMotion versions with integrated PC 960021044 InquireepBlue—epMotion PC software,960000309 Inquireoperating software for epMotion versions with integrated PC, preinstalledUpgrade and retrofit setsMC, for retrofitting an LH version into an MC version 960021002 InquireVAC, for retrofitting an LH version into a VAC version 960021011 InquireTMX, for retrofitting an LH version into a TMX version 960021055 InquireSoftware and hardware upgrade set 1,960021022 Inquireupgrade kit for epMotion 5075 with serial numbers

Automated pipetting97epMotion® automated pipetting systemOrdering informationDescription Catalog No. List USDDispensing toolsHighly precise pipetting heads, for use in the tool holder of the epMotion workstation. Each dispensing tool is completelyautoclavable at 121 °C, 1 bar for 20 min. A quality certificate for the measurement results accompanies each tool.TS 50, single-channel dispensing tool for the volume range 1–50 µL 960001010 $2,280.00TS 300, single-channel dispensing tool for the volume range 20–300 µL 960001028 2,280.00TS 1000, single-channel dispensing tool for the volume range 40–1,000 µL 960001036 2,280.00TM 50-8, 8-channel dispensing tool for the volume range 1–50 µL 960001044 4,030.00TM 300-8, 8-channel dispensing tool for the volume range 20–300 µL 960001052 4,030.00TM 1000-8, 8-channel dispensing tool for the volume range 40–1,000 µL 960001061 4,030.00Holder, for 6 dispensing tools 960001109 290.00epT.I.P.S. ® Motion pipette tipsPipette tips for automated systems in individual racks, for use with the epMotion. Tip type and size are automatically recognizedon the device. 96 epT.I.P.S./rack, 15 racks per set.Without filter, sterile50 µL, sterile, volume range 1–50 μl, 15 × 96 tips in racks 960050200 141.00300 µL, sterile, volume range 20–300 μl, 15 × 96 tips in racks 960050202 141.001,000 µL, sterile, volume range 40–1,000 μl, 15 × 96 tips in racks 960050204 141.00Without filter, Eppendorf ® Quality50 µL, volume range 1–50 µL, 15 x 96 tips in racks 960050002 120.00300 µL, volume range 20–300 µL, 15 x 96 tips in racks 960050045 120.001,000 µL, volume range 40–1,000 µL, 15 x 96 tips in racks 960050088 120.00Filtertips, sterile and PCR clean50 µL, sterile, volume range 1–50 μl, 15 × 96 tips in racks 960050201 279.00300 µL, sterile, volume range 20–300 μl, 15 × 96 tips in racks 960050203 279.001,000 µL, sterile, volume range 40–1,000 μl, 15 × 96 tips in racks 960050205 279.00Filtertips, PCR clean50 µL, volume range 1–50 µL, 15 x 96 Filtertips in racks 960050029 235.00300 µL, volume range 20–300 µL, 15 x 96 Filtertips in racks 960050061 235.001,000 µL, volume range 40–1,000 µL, 15 x 96 Filtertips in racks 960050100 235.00Reservoir rack modulesReservoir rack modules are inserted in a reservoir rack.They can be temperature controlled with a thermal module for heating and cooling.PCR 0.2 mL, for 8 x 0.2 mL PCR tubes 960002601 375.00Safe-Lock, for 4 x 0.5/1.5/2 mL tubes 960002620 390.00Tubes Ø 12 mm, for 4 x Ø 12 mm tubes 960002630 395.00Tubes Ø 16 mm, for 4 x Ø 16 mm tubes 960002640 395.0015 mL Tubes “Blue Cap,” for 4 x Ø 17 mm tubes 960002650 390.0050 mL Tubes “Blue Cap,” for 2 x Ø 29 mm tubes 960002660 395.00Reservoir adapter 30 mL, for 1 x epMotion reservoir 30 mL 960002670 157.00Reservoir adapter 100 mL, for 1 x epMotion reservoir 100 mL 960002680 157.001 | Instruments | Pipetting | DispensingPlease contact Eppendorf North America to receive a quotation for epMotion automated pipetting systems and accessories.In the U.S.: Eppendorf North America 800-645-3050 · In Canada: Eppendorf Canada Ltd. 800-263-8715E-mail: info@eppendorf.com · www.eppendorf.com

98Automated pipettingepMotion® automated pipetting system1 | Instruments | Pipetting | DispensingOrdering informationDescription Catalog No. List USDRacksFor 24 glass or plastic tubes, no temperature controlØ 17 mm x 100 mm max. length 960002024 $ 360.00Ø 17 mm x 60 mm max. length 960002156 360.00Ø 16 mm x 100 mm max. length 960002032 360.00Ø 16 mm x 60 mm max. length 960002164 360.00Ø 15 mm x 100 mm max. length 960002377 360.00Ø 15 mm x 60 mm max. length 960002326 360.00Ø 14 mm x 100 mm max. length 960002369 360.00Ø 14 mm x 60 mm max. length 960002334 360.00Ø 13 mm x 100 mm max. length 960002041 360.00Ø 13 mm x 60 mm max. length 960002342 360.00Ø 12 mm x 100 mm max. length 960002059 360.00Ø 12 mm x 60 mm max. length 960002351 360.00Rack for 24 x HPLC tubes, Ø 12 mm x 40 mm max. length 960002380 350.00Rack for 96 x 1.5/2.0 mL screw-cap tubes,960002318 920.00requires 2 positions on the deckThermorack for 24 × cryotubes ® or labelled Safe-Lock tubes 960002491 1,305.00Height adaptersFor uniform levels of labware; enables faster processing of the plate85 mm 960002105 515.0055 mm 960002113 515.0040 mm (for epT.I.P.S. ® Motion pipette tips) 960002121 515.00Accessories for epMotion 5070/5075epMotion editor, includes editor key, software package for creating and editing methods960000269 2,810.00and for printing out and archiving program sequences on a PCepMotion editor, additional license 960000300 1,095.00MultiMediaCard, for archiving parameters and transporting data960002008 87.00between control panel and PCWaste container, receptacle for used pipette tips, with lid 960002016 115.00Thermorack for 24 x 0.5 mL Safe-Lock tubes,960002067 1,045.00for a supply of 24 test tubes, temperature controlThermorack for 24 x 1.5/2 mL Safe-Lock tubes,960002075 1,045.00for a supply of 24 test tubes, temperature controlThermoadapter for Eppendorf ® Deepwell Plates 96 960002391 1,480.00Adapter sleeves, 1 set = 25 pieces960002172 330.00for reconfiguration of the thermo rack (1.5/2.0 mL) for use with 0.5 mL tubesAssembly plate, to be used with epMotion 5070 CB, supports laminar flow worktable 960002570 490.00Temperature controlled shaker, upgrade option for the 5075 LH and 5075 VAC 960021055 8,300.00Please contact Eppendorf North America to receive a quotation for epMotion automated pipetting systems and accessories.Order direct from Eppendorf North America.Product appearance, specifications, and/or prices are subject to change without notice.

Automated pipetting99epMotion® automated pipetting systemOrdering informationDescription Catalog No. List USDAccessories for PCR/real-time PCRRack Smart, for holding one tube rack of SmartCycler ® reaction tubes 960002520 $ 335.00Rack LC 20 µL/100 µL, for holding up to 96 x 20 μl or 100 μl LightCycler ® capillaries,960002511 2,550.00for use with, e.g., Centrifuge 5804/5804 R or 5810/5810 R, set of 2Thermorack CB 100 µL,960002500 1,365.00for holding up to 384 x 0.1 mL strip tubes in the Corbett Research Rotor-Gene 3000Thermoadapter for PCR plates, 96-well, skirted,960002199 805.00for use with 96 x 0.2 mL tubes or a 96-well PCR plateThermoadapter for PCR plates, 384-well, skirted,960002202 900.00for use with a 384-well PCR plateThermoadapter Frosty, combination of height adapter and960002300 790.00PCR-Cooler for cooling of skirted PCR platesThermoblock for PCR plates, 96-well,960002083 810.00for heating or cooling of PCR plates; plates are exchangeable using the gripperThermoblock for PCR plates, 384-well,960002091 899.00for heating or cooling of PCR plates; plates are exchangeable using the gripperCycleLock starter set, 1 frame and 8 mats for automated sealing of Eppendorf ® PCR plates,960002288 359.00PCR clean; can only be used with Mastercyler ® epCycleLock mats, 5 sealing mats, PCR clean, frame not included 960002296 103.00AccessoriesReservoir rack, for use with 30 mL and 100 mL reagent reservoirs,960002148 170.00holds max. seven reservoirs of 30 mL or 100 mL filling volumesepMotion ReservoirLarge volume reservoir, must be used with a reservoir rack.Two sizes: 30 mL or 100 mL maximum volume. 5 reservoirs packed in separate bags, 10 bags per set.All reservoirs are PCR clean (free of human DNA, DNase, RNase and PCR inhibitors) and made ofpolypropylene. Production batch-tested, certified.30 mL, set of 50 960051009 212.00100 mL, set of 50 960051017 309.00Accessories for nucleic acid purificationVac frame holder 960002237 515.00Vac lid 960002245 350.00Mat, for vacuum lid 960002407 228.00Vac frame 1 960002253 1,045.00Vac frame 2 960002261 1,045.00Collection Plate Adapter, for handling collection microtubes in microtube racks 960002531 127.00Channeling Plate Adapter, for vacuum-processing foaming solutions through960002540 58.00multiwell plates, set of 10Vac Thermo Lid, for effective drying of filter plates in epMotion 5075 VAC vacuum chamber960002551 1,900.00Note: additional thermal module requiredAccessories for epMotion 5075 onlyThermal module, for heating and cooling microplates and thermal racks 960002181 6,780.00Gripper, for transporting plates on the deck and for automatic operation960002270 7,815.00of the vacuum chamberGripper holder 960002211 580.001 | Instruments | Pipetting | DispensingIn the U.S.: Eppendorf North America 800-645-3050 · In Canada: Eppendorf Canada Ltd. 800-263-8715E-mail: info@eppendorf.com · www.eppendorf.com

100Service productsPipette Calibration and Repair Services1 | Service | Pipetting | DispensingFor over 65 years Eppendorf ® has been an innovator andleading provider of liquid handling systems, which includepipetting devices and consumables. This total approachphilosophy extends beyond products to our quality pipettecalibration, certification, maintenance and repair services—for all major brands of pipettes, not just our own.We offer a range of service options from basic calibrationat an economical price to GLP/GMP custom-designedcalibration services.Your results depend on the integrity of your pipettes.Trust in Eppendorf, your satisfaction is guaranteed!We deliver on our promises and your peace of mind isour utmost concern. We approach every pipette as theinstrument of your success. We know the pipette weservice today will be needed for a breakthrough discoveryor critical result. It has to be right. Attention to detail andexceptional quality and care highlight every plan we offer.Speed. Accuracy. Reliability.‡‡Full flexibility—any mix to meet your needs– Our value plans for your custom protocols– One lab or global multinational service– On site and in our state of the art facilities‡‡Full service consultation services– Technician training and seminars– Custom protocol development– Documentation management– PhysioCare ® ergonomics and pipette clinics‡‡Fully equipped and trained calibration professionals– Complete on site calibration and repair available– Proprietary Eppendorf calibration software– Environmentally robust, semi-micro,NIST traceable balances– ISO 9001:2000 RegisteredGet started today at: www.pipettecalibration.comProceduresQuickCheck PM SMInternal and external inspection of pipette 3Internal and external cleaning of pipette 3Replacement of worn or broken parts, if needed* 3Pipette calibration performed using NIST-traceable, semi-micro (0.00001g) balance –Number of measuring pointssingle-channel, fixed-volume: –single-channel, adjustable-volume: –multichannel: –Calibration label applied to pipette –Individual weighing results, mean volume, inaccuracy and imprecision valuesmeasured: –recorded: –Calibration report supplied –Single-channel manual or electronic pipette*List USD $20.00+ shippingCatalog No. 901510186Multichannel manual or electronic pipette*List USD $48.00+ shippingCatalog No. 901510187*1 Handgrip, motor and PCB are priced separately.*2 Is included in the 10 measurements*3 Standard value, deviations possible, excl. shipping duration*4 Other services may be available upon requestOrder direct from Eppendorf North America.Product appearance, specifications, and/or prices are subject to change without notice.

Service products101Online epExpress SM Pipette Calibration CenterNow it’s easier than ever to send us your pipettes forcalibration and repair. Visit our new online epExpress PipetteCalibration Center at www.pipettecalibration.com. Here youcan complete our online Service Authorization Form andget a real-time quotation based on your specific calibrationservice requirements.As a registered Eppendorf North America pipette calibration/repair customer, you will not only enjoy fast and easy onlineordering, but also gain access to a private log-in area whereyou can:1 | Service | Pipetting | Dispensing‡‡View calibration and repair service historyfor individual pipettes‡‡Get reminders for pipettes that are coming duefor calibration‡‡View your mail-in pipette service order history‡‡And more!For more information visit www.pipettecalibration.comAdvantageCalibrationAdvantageCertificationGLP/GMPCustom Calibration3 3 33 3 33 3 33 3 38 8 As requested, based on customer4 low/4 high 4 low/4 highrequirements; may include1 reading per channel low/high 1 reading per channel low/high“as found” data3 3 33 3 3– 3 3– 3 3$25.00+ shipping$35.00+ shipping901510184 901510180Determined based uponlevel of service required$60.00+ shipping$75.00+ shipping901510185 901510181Determined based uponlevel of service requiredIn the U.S.: Eppendorf North America 800-645-3050 · In Canada: Eppendorf Canada Ltd. 800-263-8715E-mail: info@eppendorf.com · www.eppendorf.com

102Service productsepMotion® Performance Plans1 | Service | Pipetting | DispensingPerformance Plan ValueChoose a plan that’s right for you. Preventive Maintenanceis at the heart of every epMotion Performance Plan. It’s aschedule of services designed to check your system andpreserve its maximum performance quality. With AdvantageService a preventive maintenance is combined with a singleservice repair visit including any parts, labor and travel, fora value packaged offering. Our Premium Service Plan isa full service agreement to cover everything on unlimitedvisits and an annual Preventive Maintenance.epMotion Tool CalibrationDispensing tools are precision devices. For this reasonthey should be regularly maintained and calibrated,which will help to ensure that they continue to performwith high precision and accuracy.Calibration of dispensing tool (gravimetric), thermal moduleand vacuum chamber S.Calibration of the dispensing tools can be performedaccording to ISO 8655 with NIST-traceable standardssupporting GLP documentation.Performance plans(1-year contract)PreventiveMaintenanceAdvantageServicePremiumCleaning and lubricating the device and components ‡ ‡ ‡Mechanical inspection and adjustment, if necessary ‡ ‡ ‡Software update (upon customer request) ‡ ‡ ‡Replacement of all dispensing tool seals ‡ ‡ ‡Function test and calibration of level sensor and‡ ‡ ‡carrier on all models; plus vacuum chamber ofepMotion 5075 VACFunction test according to service test methods ‡ ‡ ‡Full documentation ‡ ‡ ‡Number of emergency visits included 0 1 unlimitedWarranty extension (parts and labor) – 12 Months 12 monthsPreventive maintenance visits per plan year 1 1 1Please contact Eppendorf Customer Support at 800-645-3050, menu option 1, for a quotation.Order direct from Eppendorf North America.Product appearance, specifications, and/or prices are subject to change without notice.

Service products 103Long term benefits of Performance Plans‡‡Confidence in results‡‡Assurance of performance over an extended lifetime‡‡GLP complianceFeatures of Eppendorf ® Performance Plans‡‡Complete satisfaction and quality delivered byEppendorf experts‡‡Complete programs—easy to understand, easy tobudget, no surprises‡‡Complete documentation management—everythingyou need to be in complianceFor more information go to www.eppendorfna.com/service1 | Service | Pipetting | DispensingepMotion Tool Calibration Service SummaryAdvantage epMotionProceduresTool CertificationInternal/external inspection and cleaning of pipetting tool Yes YesReplacement of worn or broken parts, if needed* Yes YesYesYesPipette calibration performed using NIST-traceable,semi-micro (0.00001g) balanceNumber of measuring points:single-channel toolmultichannel tool* Spare parts additional if needed.4 low/mid/high4 low/mid/high perchannelepMotion Tool GLP/GMP CustomAs requested,based on customerrequirements;may include“as found” data.Min 4/channelMax 10/channelIndividual weighing results:measured Yes Yesrecorded Yes YesMean volume, inaccuracy and imprecision values measured and recorded Yes YesCalibration label applied to pipetting tool Yes YesCalibration report supplied Yes YesSingle-channel pipetting tool (spare parts additional if needed)Catalog No. 901510597 901510600List USD (shipping not included) $200.00(includes 4/4/4 asreturned only)Multichannel pipetting tool (spare parts additional if needed)$200.00 plus $1.00per extra readingCatalog No. 901510599 901510602List USD (shipping not included) $300.00(includes 4/4/4 asreturned only)$300.00 plus $1.00per extra readingIn the U.S.: Eppendorf North America 800-645-3050 · In Canada: Eppendorf Canada Ltd. 800-263-8715E-mail: info@eppendorf.com · www.eppendorf.com

Support and Services DirectoryContact InformationUnited StatesCanadaBusiness Hours: 8:30 a.m. to 6:00 p.m. EST 8:30 a.m. to 5:00 p.m. ESTPhone: 800-645-3050516-334-7500800-263-8715905-826-5525Fax: 516-334-7506 905-826-5424Address:Eppendorf North America, Inc.102 Motor ParkwayHauppauge, NY 11788-5178Eppendorf Canada Ltd.2810 Argentia RoadUnit #2Mississauga, ONT L5N 8L2Website: www.eppendorfna.com www.eppendorf.caEmail: info@eppendorf.com canada@eppendorf.comCustomer Support: 800-645-3050, menu option 1custserv@eppendorf.com800-263-8715, menu option 1canadacustserv@eppendorf.comTechnical Service: 800-645-3050, menu option 2techserv@eppendorf.com800-263-8715, ext. 3231canadaservice@eppendorf.com800-645-3050, menu option 2techserv@eppendorf.comwww.eppendorf.com • Email: info@eppendorf.comIn the U.S.: Eppendorf North America, Inc. 800-645-3050 • In Canada: Eppendorf Canada Ltd. 800-263-8715