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TROUBLESHOOTING TIPS - ELISA - Abcam

TROUBLESHOOTING TIPS - ELISA - Abcam

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Ensure plates are at room temperature and that the reagents are at room temperature before useConjugate too weakPrepare the substrate solutions immediately before use. Ensure the stock solutions are in date andhave been stored correctly, and are being used at the correct concentration. Ensure the reagents areused as directed, at the correct concentration.Contamination of solutionsPresence of contaminants, such as sodium azide and peroxidise can affect the substrate reaction.Avoid using reagents containing these preservatives.www.abcam.com/technical

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