The Production of Therapeutic Proteins in Plants: Apo AI and Insulin

The Production of Therapeutic Proteins in Plants: Apo AI and Insulin The Production of Therapeutic Proteins in Plants: Apo AI and Insulin

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Chemical Characterization of Purified Apo AI MilanoMS & N-terminal SequencingApo AI StandardMassExpected 28078.6 DaObserved 28076.0 DaN-terminal SequenceExpected DEPPQObserved DEPPQMassSafflower Apo AI MilanoMassExpected 27781.3 DaObserved 27780.0 DaN-terminal SequenceExpected PPQSPObserved PPQSPMass

Functional Characterization of Purified Apo AI MilanoDMPC Clearance Assay1.2001.0000.800Abs @ 325 nm0.6000.4000.2000.0000 1 2 3 4 5 6 7 8 9 10 11 12 14 16 18 20 22 24 26 28 30Time (min)DMPC (non red) ApoAI (non red) SBS Milano (non red)

Functional Characterization <strong>of</strong> Purified <strong>Apo</strong> <strong>AI</strong> MilanoDMPC Clearance Assay1.2001.0000.800Abs @ 325 nm0.6000.4000.2000.0000 1 2 3 4 5 6 7 8 9 10 11 12 14 16 18 20 22 24 26 28 30Time (m<strong>in</strong>)DMPC (non red) <strong>Apo</strong><strong>AI</strong> (non red) SBS Milano (non red)

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