Blood Sampling from an Arterial Line - Southern Health and Social ...
Blood Sampling from an Arterial Line - Southern Health and Social ... Blood Sampling from an Arterial Line - Southern Health and Social ...
SOUTHERN HEALTH & SOCIAL CARE TRUSTProcedure for Blood Sampling from an Arterial lineStatement:All those involved in the procedure for sampling from an arterial line must betrained and competent in this technique.Standard precautions should be adopted when sampling from an arterial line: ie,non-sterile gloves, and disposable apron.ANTT must be usedStop the procedure and seek medical advice if any of following are noted Resistance is felt The infants displays symptoms of pain Swelling is observed around the entry site Leakage of fluid onto the protective towel Redness around the arterial line site or along the artery Blanching of the limbEquipment:Clean trolleyProtective sheet /paper towelHand held Blood glucose analyser (as blood glucose level is often done at thistime)2 x2ml syringes, 1 x5ml syringesBlood collection bottles as required1 pre heparinised syringe2% clinnell wipe (green)Disposable apron and non -sterile gloves
SOUTHERN HEALTH & SOCIAL CARE TRUSTACTIONDecontaminate hands as per localpolicy adhering to 7 step technique and5 moments of hand hygiene. Put onPPEPrepare trolley for collection of bloodsamples using ANTT and engage anassistant to helpPlace protective paper towel in theappropriate place surrounding thearterial access deviceClean hub of red 3 way tap with Clinellwipe and allow to dry for 30 secondsConnect a 2ml syringeOpen 3 way tap to infant and syringeand off to the baby and withdraw 1 mlof blood.Turn the 3 way tap off to theinfant/infusionRemove the syringeRe-access the device using a 2 or 5 mlsyringeOpen the 3 way tap to the infant andsyringe and off to the babyWithdraw the quantity of blood requiredfor sample analysis. If blood gas isrequired re- access hub attaching thepre heparinised syringe and withdraw0.2mls of blood for blood gas analysis.Throughout the process of removal ofblood observe closely the cannula siteand surrounding area for any redness,swelling, blanching.Turn the 3 way tap off to hub. Open toinfant and infusionRemove the syringe containing theblood sample or samplesRe-attach the syringe containing wasteblood, open the 3 way tap to the infantand slowly infuse.Remove syringe and turn 3 way tap offto the hub.RATIONALETo minimize risk of infectionTo reduce risk of transmission of microorganismsand contamination of sterilefieldTo prevent soiling of cot/incubator bedand unnecessary handling of infant asa resultTo minimise risk of infectionTo facilitate blood samplingThis is waste blood which withdrawsdead spacer, clears the cannula and 3way tap of heparin, old blood and smallemboliTo obtain blood samplesTo minimise blood sampling waste
SOUTHERN HEALTH & SOCIAL CARE TRUSTACTIONDecontaminate h<strong>an</strong>ds as per localpolicy adhering to 7 step technique <strong>an</strong>d5 moments of h<strong>an</strong>d hygiene. Put onPPEPrepare trolley for collection of bloodsamples using ANTT <strong>an</strong>d engage <strong>an</strong>assist<strong>an</strong>t to helpPlace protective paper towel in theappropriate place surrounding thearterial access deviceCle<strong>an</strong> hub of red 3 way tap with Clinellwipe <strong>an</strong>d allow to dry for 30 secondsConnect a 2ml syringeOpen 3 way tap to inf<strong>an</strong>t <strong>an</strong>d syringe<strong>an</strong>d off to the baby <strong>an</strong>d withdraw 1 mlof blood.Turn the 3 way tap off to theinf<strong>an</strong>t/infusionRemove the syringeRe-access the device using a 2 or 5 mlsyringeOpen the 3 way tap to the inf<strong>an</strong>t <strong>an</strong>dsyringe <strong>an</strong>d off to the babyWithdraw the qu<strong>an</strong>tity of blood requiredfor sample <strong>an</strong>alysis. If blood gas isrequired re- access hub attaching thepre heparinised syringe <strong>an</strong>d withdraw0.2mls of blood for blood gas <strong>an</strong>alysis.Throughout the process of removal ofblood observe closely the c<strong>an</strong>nula site<strong>an</strong>d surrounding area for <strong>an</strong>y redness,swelling, bl<strong>an</strong>ching.Turn the 3 way tap off to hub. Open toinf<strong>an</strong>t <strong>an</strong>d infusionRemove the syringe containing theblood sample or samplesRe-attach the syringe containing wasteblood, open the 3 way tap to the inf<strong>an</strong>t<strong>an</strong>d slowly infuse.Remove syringe <strong>an</strong>d turn 3 way tap offto the hub.RATIONALETo minimize risk of infectionTo reduce risk of tr<strong>an</strong>smission of microorg<strong>an</strong>isms<strong>an</strong>d contamination of sterilefieldTo prevent soiling of cot/incubator bed<strong>an</strong>d unnecessary h<strong>an</strong>dling of inf<strong>an</strong>t asa resultTo minimise risk of infectionTo facilitate blood samplingThis is waste blood which withdrawsdead spacer, clears the c<strong>an</strong>nula <strong>an</strong>d 3way tap of heparin, old blood <strong>an</strong>d smallemboliTo obtain blood samplesTo minimise blood sampling waste
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