GABA - Molecular Devices

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Summary and Conclusions• Electrophysiological assays for screening compounds against <strong>GABA</strong> Areceptors have been developed using two automated platforms andMillipore’s and cell lines.• These assays can detect agonists, antagonists and allosteric modulatorswith good resolution and reproducibility and can discriminate subtypeselective compounds• Despite the inability to resolve peak agonist responses, these datademonstrate that assays for improved throughput compound screeningagainst <strong>GABA</strong> Achannels can be developed on Ionworks• Using a similar approach, assays for other slowly gating LGICs are beingdeveloped, providing the opportunity for information-richelectrophysiological screening of compounds earlier in the screeningcascade
AcknowledgementsMillipore Ion Channel Group, Cambridge, UK:Umesh Patel Ray Helliwell Ed MaughlingLuanda Pym Reena Reehal Jonathan MannToni Cooper Helen Swain Louise WebdaleCelia Holdsworth Andrew CookCell line generation and assay developmentwas partly carried out incollaboration with GSK, Stevenage, UK
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