GABA - Molecular Devices

Use of Ionworks for ligand-gated channel profiling‣ Ligand-gated channels provide technical challenges for high throughputscreening with Ionworks:– rapid activation and desensitisation requires minimal delay between ligand addition and currentrecording - limited by independent electronics and fluidics heads– no compound washout so control responses and different concentrations have to be compared inseparate wells (c.f. PatchXpress, manual patch clamp where same cell can be used, i.e. intra-wellcontrols)‣ Improvements with Iwks Quattro now allow possibility of recording ligand-gatedchannels with slower kinetics– 48 channel fluidics head (shorter delay before E-head can record)– dual compound addition capability (e.g. for measuring antagonists)– greater well-to-well reproducibility of PPC - allows more reliable inter-well comparisonGABA6 sCurrent measured by IonWorks Quattro2 nA5 s(Trace from PatchXpress: V H= -60 mV)