PEGRx HT ™ - HR2-086 Scoring Sheet - Hampton Research

Sample:Sample Buffer:Reservoir Volume:Sample Concentration:Date:Temperature:Drop Volume: Total ml Sample ml Reservoir ml Additive ml1 Clear Drop2 Phase Separation3 Regular Granular Precipitate4 Birefringent Precipitate orMicrocrystals5 Posettes or Spherulites6 Needles (1D Growth)7 Plates (2D Growth)8 Single Crystals (3D Growth < 0.2 mm)9 Single Crystals (3D Growth > 0.2 mm)PEGRx HT - HR2-086 Scoring Sheet Date: Date: Date: Date:1. (A1) 0.1 M Citric acid pH 3.5, 34% v/v Polyethylene glycol 2002. (A2) 0.1 M Sodium citrate tribasic dihydrate pH 5.5, 38% v/v Polyethylene glycol 2003. (A3) 0.1 M HEPES pH 7.5, 42% v/v Polyethylene glycol 2004. (A4) 0.1 M Sodium acetate trihydrate pH 4.5, 30% v/v Polyethylene glycol 3005. (A5) 0.1 M BIS-TRIS pH 6.5, 25% v/v Polyethylene glycol 3006. (A6) 0.1 M BICINE pH 8.5, 20% v/v Polyethylene glycol 3007. (A7) 0.1 M Sodium acetate trihydrate pH 4.0, 15% v/v Polyethylene glycol 4008. (A8) 0.1 M MES monohydrate pH 6.0, 22% v/v Polyethylene glycol 4009. (A9) 0.1 M Tris pH 8.0, 30% v/v Polyethylene glycol 40010. (A10) 0.1 M Sodium citrate tribasic dihydrate pH 5.0, 30% v/v Polyethylene glycol monomethyl ether 55011. (A11) 0.1 M Imidazole pH 7.0, 25% v/v Polyethylene glycol monomethyl ether 55012. (A12) 0.1 M BIS-TRIS propane pH 9.0, 20% v/v Polyethylene glycol monomethyl ether 55013. (B1) 0.1 M Sodium acetate trihydrate pH 4.0, 10% v/v Jeffamine ® M-600 ® pH 7.014. (B2) 0.1 M MES monohydrate pH 6.0, 20% v/v Jeffamine ® M-600 ® pH 7.015. (B3) 0.1 M Tris pH 8.0, 30% v/v Jeffamine ® M-600 ® pH 7.016. (B4) 0.1 M Citric acid pH 3.5, 14% w/v Polyethylene glycol 1,00017. (B5) 0.1 M Sodium citrate tribasic dihydrate pH 5.5, 22% w/v Polyethylene glycol 1,00018. (B6) 0.1 M HEPES pH 7.5, 30% w/v Polyethylene glycol 1,00019. (B7) 0.1 M Sodium acetate trihydrate pH 4.5, 30% w/v Polyethylene glycol 1,50020. (B8) 0.1 M BIS-TRIS pH 6.5, 20% w/v Polyethylene glycol 1,50021. (B9) 0.1 M BICINE pH 8.5, 15% w/v Polyethylene glycol 1,50022. (B10) 0.1 M Sodium acetate trihydrate pH 4.0, 10% w/v Polyethylene glycol monomethyl ether 2,00023. (B11) 0.1 M MES monohydrate pH 6.0, 20% w/v Polyethylene glycol monomethyl ether 2,00024. (B12) 0.1 M Tris pH 8.0, 30% w/v Polyethylene glycol monomethyl ether 2,00025. (C1) 0.1 M Sodium citrate tribasic dihydrate pH 5.0, 30% v/v Jeffamine ® ED-2001 pH 7.026. (C2) 0.1 M Imidazole pH 7.0, 20% v/v Jeffamine ® ED-2001 pH 7.027. (C3) 0.1 M BIS-TRIS propane pH 9.0, 10% v/v Jeffamine ® ED-2001 pH 7.028. (C4) 0.1 M Citric acid pH 3.5, 25% w/v Polyethylene glycol 3,35029. (C5) 0.1 M Sodium citrate tribasic dihydrate pH 5.5, 18% w/v Polyethylene glycol 3,35030. (C6) 0.1 M HEPES pH 7.5, 12% w/v Polyethylene glycol 3,35031. (C7) 0.1 M Sodium acetate trihydrate pH 4.0, 10% w/v Polyethylene glycol 4,00032. (C8) 0.1 M MES monohydrate pH 6.0, 14% w/v Polyethylene glycol 4,00033. (C9) 0.1 M Tris pH 8.0, 28% w/v Polyethylene glycol 4,00034. (C10) 0.1 M Sodium acetate trihydrate pH 4.5, 30% w/v Polyethylene glycol monomethyl ether 5,00035. (C11) 0.1 M BIS-TRIS pH 6.5, 20% w/v Polyethylene glycol monomethyl ether 5,00036. (C12) 0.1 M BICINE pH 8.5, 8% w/v Polyethylene glycol monomethyl ether 5,00037. (D1) 0.1 M Sodium citrate tribasic dihydrate pH 5.0, 10% w/v Polyethylene glycol 6,00038. (D2) 0.1 M Imidazole pH 7.0, 20% w/v Polyethylene glycol 6,00039. (D3) 0.1 M BIS-TRIS propane pH 9.0, 30% w/v Polyethylene glycol 6,00040. (D4) 0.1 M Citric acid pH 3.5, 28% w/v Polyethylene glycol 8,00041. (D5) 0.1 M Sodium citrate tribasic dihydrate pH 5.5, 16% w/v Polyethylene glycol 8,00042. (D6) 0.1 M HEPES pH 7.5, 4% w/v Polyethylene glycol 8,00043. (D7) 0.1 M Sodium acetate trihydrate pH 4.5, 10% w/v Polyethylene glycol 10,00044. (D8) 0.1 M BIS-TRIS pH 6.5, 16% w/v Polyethylene glycol 10,00045. (D9) 0.1 M BICINE pH 8.5, 20% w/v Polyethylene glycol 10,00046. (D10) 0.1 M Sodium citrate tribasic dihydrate pH 5.0, 18% w/v Polyethylene glycol 20,00047. (D11) 0.1 M Imidazole pH 7.0, 12% w/v Polyethylene glycol 20,00048. (D12) 0.1 M BIS-TRIS propane pH 9.0, 8% w/v Polyethylene glycol 20,000HR2-086 - Page 1 of 2

Sample:Sample Buffer:Reservoir Volume:Sample Concentration:Date:Temperature:Drop Volume: Total ml Sample ml Reservoir ml Additive ml1 Clear Drop2 Phase Separation3 Regular Granular Precipitate4 Birefringent Precipitate orMicrocrystals5 Posettes or Spherulites6 Needles (1D Growth)7 Plates (2D Growth)8 Single Crystals (3D Growth < 0.2 mm)9 Single Crystals (3D Growth > 0.2 mm)PEGRx HT - HR2-086 Scoring Sheet Date: Date: Date:49. (E1) 0.8 M Lithium sulfate monohydrate, 0.1 M Sodium acetate trihydrate pH 4.0, 4% v/v Polyethylene glycol 20050. (E2) 0.2 M Lithium sulfate monohydrate, 0.1 M Sodium citrate tribasic dihydrate pH 5.0, 26% v/v Polyethylene glycol 20051. (E3) 0.05 M Calcium chloride dihydrate, 0.1 M MES monohydrate pH 6.0, 45% v/v Polyethylene glycol 20052. (E4) 28% v/v 2-Propanol, 0.1 M BIS-TRIS pH 6.5, 3% v/v Polyethylene glycol 20053. (E5) 20% v/v Tacsimate pH 7.0, 0.1 M HEPES pH 7.5, 2% v/v Polyethylene glycol 20054. (E6) 10% v/v 2-Propanol, 0.1 M Sodium citrate tribasic dihydrate pH 5.0, 26% v/v Polyethylene glycol 40055. (E7) 0.2 M Ammonium acetate, 0.1 M Sodium citrate tribasic dihydrate pH 5.5, 24% v/v Polyethylene glycol 40056. (E8) 0.2 M Ammonium sulfate, 0.1 M BIS-TRIS pH 6.5, 18% v/v Polyethylene glycol 40057. (E9) 0.19 mM CYMAL ® -7, 0.1 M HEPES pH 7.5, 40% v/v Polyethylene glycol 40058. (E10) 6% v/v 2-Propanol, 0.1 M Sodium acetate trihydrate pH 4.5, 26% v/v Polyethylene glycol monomethyl ether 55059. (E11) 1.8 M Ammonium sulfate, 0.1 M BIS-TRIS pH 6.5, 2% v/v Polyethylene glycol monomethyl ether 55060. (E12) 0.15 M DL-Malic acid pH 7.0, 0.1 M Imidazole pH 7.0, 22% v/v Polyethylene glycol monomethyl ether 55061. (F1) 0.1 M Succinic acid pH 7.0, 0.1 M BICINE pH 8.5, 30% v/v Polyethylene glycol monomethyl ether 55062. (F2) 0.1 M Lithium sulfate monohydrate, 0.1 M Sodium citrate tribasic dihydrate pH 5.5, 20% w/v Polyethylene glycol 1,00063. (F3) 0.1 M Sodium malonate pH 8.0, 0.1 M Tris pH 8.0, 30% w/v Polyethylene glycol 1,00064. (F4) 4% v/v (+/-)-2-Methyl-2,4-pentanediol, 0.1 M Citric acid pH 3.5, 20% w/v Polyethylene glycol 1,50065. (F5) 0.2 M L-Proline, 0.1 M HEPES pH 7.5, 24% w/v Polyethylene glycol 1,50066. (F6) 10% v/v 2-Propanol, 0.1 M BICINE pH 8.5, 30% w/v Polyethylene glycol 1,50067. (F7) 0.1 M Sodium chloride, 0.1 M BIS-TRIS propane pH 9.0, 25% w/v Polyethylene glycol 1,50068. (F8) 0.02 M Nickel(II) chloride hexahydrate, 0.02 M Magnesium chloride hexahydrate, 0.02 M Cadmium chloride hydrate,0.1 M Sodium acetate trihydrate pH 4.5, 24% w/v Polyethylene glycol monomethyl ether 2,00069. (F9) 20% v/v 2-Propanol, 0.1 M MES monohydrate pH 6.0, 20% w/v Polyethylene glycol monomethyl ether 2,00070. (F10) 0.2 M Ammonium citrate tribasic pH 7.0, 0.1 M Imidazole pH 7.0, 20% w/v Polyethylene glycol monomethyl ether 2,00071. (F11) 4.0 M Potassium formate, 0.1 M BIS-TRIS propane pH 9.0, 2% w/v Polyethylene glycol monomethyl ether 2,00072. (F12) 50% v/v Tacsimate pH 4.0, 0.1 M Sodium acetate trihydrate pH 4.5, 1% w/v Polyethylene glycol 3,35073. (G1) 0.10% w/v n-Octyl-b-D-glucoside, 0.1 M Sodium citrate tribasic dihydrate pH 5.5, 22% w/v Polyethylene glycol 3,35074. (G2) 2% v/v Tacsimate pH 7.0, 5% v/v 2-Propanol, 0.1 M Imidazole pH 7.0, 8% w/v Polyethylene glycol 3,35075. (G3) 2% v/v 1,4-Dioxane, 0.1 M Tris pH 8.0, 15% w/v Polyethylene glycol 3,35076. (G4) 18% v/v 2-Propanol, 0.1 M Sodium citrate tribasic dihydrate pH 5.5, 20% w/v Polyethylene glycol 4,00077. (G5) 6% v/v Tacsimate pH 6.0, 0.1 M MES monohydrate pH 6.0, 25% w/v Polyethylene glycol 4,00078. (G6) 0.2 M Magnesium formate dihydrate, 0.1 M Sodium acetate trihydrate pH 4.0, 18% w/v Polyethylene glycol monomethyl ether 5,00079. (G7) 2% v/v Polyethylene glycol 400, 0.1 M Imidazole pH 7.0, 24% w/v Polyethylene glycol monomethyl ether 5,00080. (G8) 0.2 M Sodium formate, 0.1 M BICINE pH 8.5, 20% w/v Polyethylene glycol monomethyl ether 5,00081. (G9) 4% v/v 2-Propanol, 0.1 M BIS-TRIS propane pH 9.0, 20% w/v Polyethylene glycol monomethyl ether 5,00082. (G10) 6% v/v Ethylene glycol, 0.1 M Citric acid pH 3.5, 10% w/v Polyethylene glycol 6,00083. (G11) 0.15 M Lithium sulfate monohydrate, 0.1 M Citric acid pH 3.5, 18% w/v Polyethylene glycol 6,00084. (G12) 10% v/v 2-Propanol, 0.1 M Sodium acetate trihydrate pH 4.0, 22% w/v Polyethylene glycol 6,00085. (H1) 0.2 M Sodium chloride, 0.1 M Sodium acetate trihydrate pH 4.0, 22% w/v Polyethylene glycol 8,00086. (H2) 20% v/v 2-Propanol, 0.1 M Tris pH 8.0, 5% w/v Polyethylene glycol 8,00087. (H3) 10% v/v Polyethylene glycol 200, 0.1 M BIS-TRIS propane pH 9.0, 18% w/v Polyethylene glycol 8,00088. (H4) 15% v/v 2-Propanol, 0.1 M Sodium citrate tribasic dihydrate pH 5.0, 10% w/v Polyethylene glycol 10,00089. (H5) 0.4 M Sodium malonate pH 6.0, 0.1 M MES monohydrate pH 6.0, 0.5% w/v Polyethylene glycol 10,00090. (H6) 0.2 M Potassium sodium tartrate tetrahydrate, 0.1 M BIS-TRIS pH 6.5, 10% w/v Polyethylene glycol 10,00091. (H7) 5% v/v (+/-)-2-Methyl-2,4-pentanediol, 0.1 M HEPES pH 7.5, 10% w/v Polyethylene glycol 10,00092. (H8) 0.2 M Ammonium acetate, 0.1 M Tris pH 8.0, 16% w/v Polyethylene glycol 10,00093. (H9) 5% v/v 2-Propanol, 0.1 M Citric acid pH 3.5, 6% w/v Polyethylene glycol 20,00094. (H10) 1.0 M Sodium malonate pH 5.0, 0.1 M Sodium acetate trihydrate pH 4.5, 2% w/v Polyethylene glycol 20,00095. (H11) 0.2 M Magnesium chloride hexahydrate, 0.1 M Sodium citrate tribasic dihydrate pH 5.0, 10% w/v Polyethylene glycol 20,00096. (H12) 3% w/v Dextran sulfate sodium salt, 0.1 M BICINE pH 8.5, 15% w/v Polyethylene glycol 20,000HR2-086 - Page 2 of 2