Ordinance and Syllabus - Bhagat Phool Singh Mahila ...
Ordinance and Syllabus - Bhagat Phool Singh Mahila ...
Ordinance and Syllabus - Bhagat Phool Singh Mahila ...
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Paper Code: BPL-206Pharmaceutics-V (Pharmaceutical Microbiology)L -- T -- P Total Credits: 044 -- -- Total Marks: 100External Marks: 50Internal Marks: 50Paper Objectives:Students are taught the principles of Microbiology with special reference to PharmaceuticalMicrobiology including environmental Microbiology <strong>and</strong> other relevant aspects. The modernconcepts of microbiological application are taught to students. The staining of slides <strong>and</strong>preparation of culture media, etc., including microbiological assays of pharmaceuticals is alsotaught. Sensitivity test <strong>and</strong> other necessary pharmaceutical tests are also included. Thestudents are trained about sterilization, disinfection <strong>and</strong> fermentation with reference to theiruse <strong>and</strong> application in the pharmaceutical industry. Knowledge of immune system is alsoimparted.UNIT IIntroduction to the science of microbiology: ancient theories concerning the originof life, contribution of great scientists to this science, with particular reference to thecontributions of the following scientists: A.V. Leeuwenhoek, Louis Pasteur, EdwardJenner, Robert Koch, Alex<strong>and</strong>er Fleming, Joseph Lister.Microscopy: Microscopes, their magnification, resolution, illumination <strong>and</strong> filters,working of different types of microscopes, micrometry.UNIT IIClassification of microbes <strong>and</strong> their taxonomy.Bacteria, fungi <strong>and</strong> viruses: Nutrition, cultivation, isolation <strong>and</strong> identification.Bacterial enzymes – classification, nomenclature, production by fermentation,extraction methods, immobilization techniques <strong>and</strong> applications of bacterial enzymesin general <strong>and</strong> detailed account of following bacterial enzymes: alpha amylase(diastase) <strong>and</strong> proteases.UNIT IIIDisinfection: factors affecting disinfection, dynamics of disinfection, disinfectants<strong>and</strong> antiseptics <strong>and</strong> their evaluation.Sterilization: Different methods, applications <strong>and</strong> evaluation of sterilization methods,aseptic technique.UNIT IVMicrobial st<strong>and</strong>ardization: Microbial st<strong>and</strong>ardization of antibiotics (ampicillin,streptomycin), Vitamins (Vitamin B-12, Niacin) <strong>and</strong> calcium pentothenate.Fermentation: Types of media used; factors affecting, control of various parametersduring fermentation. A detailed account of the industrial fermentation process formanufacture of penicillin, streptomycin, glutamic acid; Lysine, citric acid, Vit.B 12 .65
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