Calibration Procedure - PDF

First Standard: 100 ppm Add 10-ml of solvent tothe 10-ml graduatedcylinder Using the syringe add10 microliters of oil to 10-ml of solvent in thegraduated cylinder

First Standard: 100 ppm Add 10-ml of solvent tothe 10-ml graduatedcylinder Using the syringe add10 microliters of oil to 10-ml of solvent in thegraduated cylinder Pour this standard intoa vial labeled “100 ppm” Shake to mix

Second Standard: 50 ppmPour 5-ml of the 100 ppm standard back into the10-ml graduated cylinder

Second Standard: 50 ppmPour 5-ml of the 100 ppm standard back into the10-ml graduated cylinderFill the cylinder to 10-ml with clean solvent

Second Standard: 50 ppmPour 5-ml of the 100 ppm standard back into the10-ml graduated cylinderFill the cylinder to 10-ml with clean solvent This will cut the 100 ppm standard in half to be 50ppmPour this standard into a vial labeled “50 ppm”Shake to mix

Third Standard: 25 ppmPour 5-ml of the 50 ppm standard back into the10-ml graduated cylinderFill the cylinder to 10-ml with clean solvent

Third Standard: 25 ppmPour 5-ml of the 50 ppm standard back into the10-ml graduated cylinderFill the cylinder to 10-ml with clean solventThis will cut the 50 ppm standard in half to be 25 ppmPour this standard into a vial labeled “25 ppm”

Calibration to Prepared StandardsMake sure InfraCal has been on for1 hourMake sure cuvette is cleanZero InfraCal

ZeroingFill the cuvette with clean solvent and place inthe sample stage with the frosted side facingfront.

ZeroingPress and hold the ZERO button until thedisplay reads bAL.bALRUNCALZERORecall

ZeroingA multiplier value to 3 decimal places will bedisplayed when zero is established. The actualvalue is only of interest when reportingproblems to the factory1.267RUNCALZERORecall

Zeroing Press RUN. The display should read 00 + 02If not, repeat the zero process00RUNCALZERORecall

Calibration to Prepared StandardsRun each standard at least 3 times on theInfraCalAverage results for each standardEnter averaged results under the “A” in themanual on page 13 or 14 and thecorresponding calibration value in the Ccolumn

Example Calibration TableAAbsorbanceCCalibrationStandardAO1= 42 CO1= 25AO2= 81 CO2= 50AO3= 149 CO3= 100

Entering Values into EDIT programMake sure Cal Lock switch on the back of the InfraCal isin the “I” positionEN 61010-1EN 55022 Class BEN 50082-1+Serial #Made In U.S.A.

Press the CAL button for two seconds,until CAL appears on the displayCALRUNCALZERORecall

Press RECALL until Edit is displayedEditRUNCALZERORecall

Press CAL and the display will read n=for a short timen=33RUNCALZERORecall

Set n= to the number standards using theup and down arrowsn=33RUNCALZERORecall

Set n= to the number standards using theup and down arrows (for our example itwill be 3)3RUNCALZERORecall

Press CAL and the display will read A01=for a short timeA01RUNCALZERORecall

Using the up and down arrows, scroll to thefirst absorbance value (for our example it will be42)AbsorbanceCalibrationStandardAO1= 42 CO1= 25AO2= 81 CO2= 50AO3= 149 CO3= 100

Using the up and down arrows, scroll to thefirst absorbance value (for our example it will be42)42RUNCALZERORecall

Press CAL again and the display will read C01=C01RUNCALZERORecall

Using the up and down arrows, scroll to the firstcalibration value (for our example it will be 25)AbsorbanceCalibrationStandardAO1= 42 CO1= 25AO2= 81 CO2= 50AO3= 149 CO3= 100

Using the up and down arrows, scroll to the firstcalibration value (for our example it will be 25)25RUNCALZERORecall

Press CAL and the display will read A02= for ashort time. Using the up and down arrows,scroll to the first calibration value (for ourexample it will be 81)AbsorbanceCalibrationStandardAO1= 42 CO1= 25AO2= 81 CO2= 50AO3= 149 CO3= 100

Press CAL again and the display will read C02=Scroll this to the second calibration valueAbsorbanceCalibrationStandardAO1= 42 CO1= 25AO2= 81 CO2= 50AO3= 149 CO3= 100

Do the same for AO3 and CO3AbsorbanceCalibrationStandardAO1= 42 CO1= 25AO2= 81 CO2= 50AO3= 149 CO3= 100

Press CAL again and the display will read IDLEIDLERUNCALZERORecallThe calibration is now stored andthe calibration is now active in the InfraCal

InfraCal TOG/TPH Analyzer,Model CVHCalibrationFor questions please contact us at:tech@WilksIR.com, or 203-855-9136