Inertsil ODS-3 - Winlab.com.au
Inertsil ODS-3 - Winlab.com.au
Inertsil ODS-3 - Winlab.com.au
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Introduction of a new product from®<strong>Inertsil</strong> Series
How to selectColumn packing* Difference and features of <strong>ODS</strong>* The First choice <strong>ODS</strong> columnswww.winlab.<strong>com</strong>.<strong>au</strong> Ph 61 07 3205 5233Fax 61 07 3205 1209 email: info@winlab.<strong>com</strong>.<strong>au</strong>1
*2 There are many kinds of <strong>ODS</strong>, do you know howdifference is?Waters - Acquity , Symmetry , Atlantis , etc.Agilent - Zorbax eclipse , zorbax extend , etc.Phenomenex- Luna , Gemini , Jupiter , Synergi , etcGLS- <strong>Inertsil</strong> <strong>ODS</strong>-4, <strong>ODS</strong>-3, <strong>ODS</strong>-P , <strong>ODS</strong>-SP , etcThere are many kinds of <strong>ODS</strong>.Chromatograms are difference even if the analytical conditions were the samewith the different columns.0 2 4 6Time (min)0 2 4 6Time (min)
<strong>Inertsil</strong> Series <strong>ODS</strong> columnsSpecification of various <strong>ODS</strong> columnsPacking Material<strong>Inertsil</strong> <strong>ODS</strong>-4<strong>Inertsil</strong> <strong>ODS</strong>-3<strong>Inertsil</strong> <strong>ODS</strong>-P<strong>Inertsil</strong> <strong>ODS</strong>-EP<strong>Inertsil</strong> <strong>ODS</strong>-SP<strong>Inertsil</strong> <strong>ODS</strong>-2<strong>Inertsil</strong> <strong>ODS</strong>-80ABase Silica gel (Spherical)SurfaceArea450m 2 /g450m 2 /g450m 2 /g450m 2 /g450m 2 /g320m 2 /g450m 2 /gPoreSize100 Å100Å100Å100Å100Å150ÅPurity99.999%99.999%99.999%99.999%99.999%99.999%CarbonLoadingChemical TreatmentBonded-phaseStructureEnd-capping11% Monomeric ★★★★★★15% Monomeric ★★★★★29% Polymeric No9% Embedded No8.5% Monomeric ★★★★18.5% Intermediate ★★★★80Å 99.99% 17.5% Monomeric ★★★★www.winlab.<strong>com</strong>.<strong>au</strong> Ph 61 07 3205 5233Fax 61 07 3205 1209 email: info@winlab.<strong>com</strong>.<strong>au</strong>3
Retention time is effected by the carbon loading<strong>Inertsil</strong> <strong>ODS</strong>-3Carbon loading:15%<strong>Inertsil</strong> <strong>ODS</strong>-4 11230 20Time (min)23 445 65677ConditionsColumnEluent: <strong>ODS</strong>(5 um, 250 x 4.6 mm I.D.): A) CH 3 OHB) H 2 OA/B = 80/20 , v/v: 1.0 mL/min.Flow rateCol. Temp. : 40 ℃Detection : UV 254 nmCarbon loading: 11%<strong>Inertsil</strong> <strong>ODS</strong>-SPCarbon loading: 8.5%0 20Time (min)721 34561) Uracil2) Caffein3) Phenol4) n-Butylbenzene5) o-Terphenyl6) n-Amylbenzene7) Triphenylene0 20Time (min)If the carbon loading is bigger, it trends to longer retention time.
<strong>Inertsil</strong> Series <strong>ODS</strong> columnsSpecification of various <strong>ODS</strong> columnsPacking Material<strong>Inertsil</strong> <strong>ODS</strong>-4<strong>Inertsil</strong> <strong>ODS</strong>-3<strong>Inertsil</strong> <strong>ODS</strong>-P<strong>Inertsil</strong> <strong>ODS</strong>-EP<strong>Inertsil</strong> <strong>ODS</strong>-SP<strong>Inertsil</strong> <strong>ODS</strong>-2<strong>Inertsil</strong> <strong>ODS</strong>-80ABase Silica gel (Spherical)SurfaceArea450m 2 /g450m 2 /g450m 2 /g450m 2 /g450m 2 /g320m 2 /g450m 2 /gPoreSize100 Å100Å100Å100Å100Å150ÅPurity99.999%99.999%99.999%99.999%99.999%99.999%CarbonLoadingChemical TreatmentBonded-phaseStructureEnd-capping11% Monomeric ★★★★★★15% Monomeric ★★★★★29% Polymeric No9% Embedded No8.5% Monomeric ★★★★18.5% Intermediate ★★★★80Å 99.99% 17.5% Monomeric ★★★★www.winlab.<strong>com</strong>.<strong>au</strong> Ph 61 07 3205 5233Fax 61 07 3205 1209 email: info@winlab.<strong>com</strong>.<strong>au</strong>5
Analysis of CaroteneCH 3CH 3 CH 3 CH 3H 3 CCH 3CH 3CH 3alpha -caroteneCH 3CH 3CH 3CH 3 CH 3 CH 3H 3 CCH 3CH 3CH 3Different ofstructureConditionsColumn : <strong>ODS</strong>(5 um, 250 x 4.6 mm I.D.)Eluent : A) CH 3 OHB) C 2 H 5 OHFlow rate : 1.0 mL/min.Col. Temp. : 40 ℃Detection : UV 455 nmInjection vol. : 20 uLbeta-caroteneCH 3CH 31. α-Carotene ( 2.0mg/L )2. β-Carotene ( 2.0mg/L )mAU0 2 4 6 8 10 12 14Polymeric <strong>ODS</strong>(<strong>Inertsil</strong> <strong>ODS</strong>-P)1 2A/B = 90/10, v/v0 10 20Ti me ( mi n)mAU0 2 4 6 8 10 12 14Monomeric <strong>ODS</strong>(<strong>Inertsil</strong> <strong>ODS</strong>-3)12A/B = 45/55, v/v0 10 20Ti me ( mi n)
Monomeric <strong>ODS</strong> vs Polymeric <strong>ODS</strong>Silica-gel surfacePlanar<strong>com</strong>poundsC18 groupMonomeric <strong>ODS</strong>Non-planar<strong>com</strong>poundsPolymeric <strong>ODS</strong>www.winlab.<strong>com</strong>.<strong>au</strong> Ph 61 07 3205 5233Fax 61 07 3205 1209 email: info@winlab.<strong>com</strong>.<strong>au</strong>: Hydrophobic interaction
<strong>Inertsil</strong> Series <strong>ODS</strong> columnsSpecification of various <strong>ODS</strong> columnsPacking Material<strong>Inertsil</strong> <strong>ODS</strong>-4<strong>Inertsil</strong> <strong>ODS</strong>-3<strong>Inertsil</strong> <strong>ODS</strong>-P<strong>Inertsil</strong> <strong>ODS</strong>-EP<strong>Inertsil</strong> <strong>ODS</strong>-SP<strong>Inertsil</strong> <strong>ODS</strong>-2<strong>Inertsil</strong> <strong>ODS</strong>-80ABase Silica gel (Spherical)SurfaceArea450m 2 /g450m 2 /g450m 2 /g450m 2 /g450m 2 /g320m 2 /g450m 2 /gPoreSize100 Å100Å100Å100Å100Å150ÅPurity99.999%99.999%99.999%99.999%99.999%99.999%CarbonLoadingChemical TreatmentBonded-phaseStructureEnd-capping11% Monomeric ★★★★★★15% Monomeric ★★★★★29% Polymeric No9% Embedded No8.5% Monomeric ★★★★18.5% Intermediate ★★★★80Å 99.99% 17.5% Monomeric ★★★★www.winlab.<strong>com</strong>.<strong>au</strong> Ph 61 07 3205 5233Fax 61 07 3205 1209 email: info@winlab.<strong>com</strong>.<strong>au</strong>8
*2 There are many kinds of <strong>ODS</strong>, do you know howdifference is?Waters - Acquity , Symmetry , Atlantis , etc.Agilent - Zorbax eclipse , zorbax extend , etc.Phenomenex- Luna , Gemini , Jupiter , Synergi , etcGLS- <strong>Inertsil</strong> <strong>ODS</strong>-4, <strong>ODS</strong>-3, <strong>ODS</strong>-P , <strong>ODS</strong>-SP , etcThere are many kinds of <strong>ODS</strong>.Chromatograms are difference even if the analytical conditions were the samewith the different columns.0 2 4 6Time (min)0 2 4 6Time (min)
Adsorption of analyte (1)* Basic Compounds :* Acidic Compounds :C<strong>au</strong>sed by residual hydroxyl group(silanol) on silica surface.C<strong>au</strong>sed by reaction reagent.These reagent includes amine groupfor the purpose of resistance tobasic <strong>com</strong>pounds adsorption.Adsorption mechanismof basic <strong>com</strong>poundsC18C18C18C18C18C18OHOH OH OH OH OHOHOHSi SiO SiOSi OOOSilica<strong>ODS</strong>OHSiOOH OHSi SiOOOSilicaOHSiOOHEnd-CappingC1SiOC1 OHSi SiOO OSilicaOC1SiC1www.winlab.<strong>com</strong>.<strong>au</strong> Ph 61 07 3205 5233Fax 61 07 3205 1209 email: info@winlab.<strong>com</strong>.<strong>au</strong>10
Worse peak shape is c<strong>au</strong>sed by adsorption of thepacking material.1Column :<strong>ODS</strong> 5um, 4.6×250 mmEluent :CH 3 OH / H2O = 30 / 70Flow Rate :1.0 mL/minCol.Temp. :40 ℃Detector :UV254 nmSample Size : 4 uLSamples :1)Pyridine 0.09 mg/mL2 2)Phenol 0.41 mg/mL210 2 4 6 8 10 12 14 16 18Time (min)<strong>Inertsil</strong> <strong>ODS</strong>-3V0 2 4 6 8 10 12 14 16 18Time (min)S:Symmetry C18www.winlab.<strong>com</strong>.<strong>au</strong> Ph 61 07 3205 5233Fax 61 07 3205 1209 email: info@winlab.<strong>com</strong>.<strong>au</strong>
<strong>Inertsil</strong> Series HPLC Column・High Purity, Spherical Silica-gel→ High Theoretical Plates・Highly Uniformed Particle Size Distribution → Low Back Pressure・Highly Stable Chemical Bonding→ High Durability・Extreme Endcapping Treatment→ Lower Adsorption・Rigorous Inspection Tests (More than 10 Tests) → Batch-to-Batch ReproducibilityThe biggest feature is…We synthesize the material of thesilica-gel and conduct chemicalbonding, inspection and thepacking of the column by ourselves.Highly StableExcellent SupportContinue to Evolvewww.winlab.<strong>com</strong>.<strong>au</strong> Ph 61 07 3205 523312Fax 61 07 3205 1209 email: info@winlab.<strong>com</strong>.<strong>au</strong>
Highly Pure Base Silica-gel・ Completely spherical・ Smooth surface・ Synthesized from pure ingredients・ Focused only for chromatography・ Whole manufacturing process within GLS<strong>Inertsil</strong> Silica-gelwww.winlab.<strong>com</strong>.<strong>au</strong> Ph 61 07 3205 523313Fax 61 07 3205 1209 email: info@winlab.<strong>com</strong>.<strong>au</strong>One <strong>com</strong>petitor's Silica-gel
hat makes the column back pressure different ?It depends on the…size of the silica geluniformity of the particle sizeparticle shape5μmUniformedSphericalBack pressure = Low3μmUniformedSphericalBack pressure = High5μmNot uniformedSphericalBack pressure=High5μmUniformedIrregularBack pressure=High14
What makes the column back pressure different?Reasons why <strong>Inertsil</strong> can offer the lowest back pressure column in the world!Packing-Low back pressure-High theoretical plates-Lot-to-Lot reproducibility<strong>Inertsil</strong>Packing-High back pressure-Low theoretical plates-Poor Lot-to-Lot reproducibility15Competitor Y’s silica gelwww.winlab.<strong>com</strong>.<strong>au</strong> Ph 61 07 3205 5233Fax 61 07 3205 1209 email: info@winlab.<strong>com</strong>.<strong>au</strong>
Back pressure of <strong>Inertsil</strong> is very lowWhen selecting a low-pressure column…Easy to reduce running costs.Can keep yourself healthy.Back pressure of <strong>com</strong>mercially available columns200Pressure (kgf/cm2)180160140120100806040●:<strong>Inertsil</strong> <strong>ODS</strong>-3●:<strong>Inertsil</strong> <strong>ODS</strong>-40 20 40 60 80 100CH3OH conc.(%)16Analytical ConditionsColumn Size: 250x4.6mmIDFlow Rate: 1.0 mL/minCol.Temp. : 40C
Press. (MPa)Back pressure of <strong>com</strong>mercially available columns16Column size : 5μm, 250×4.6mmI.D.Eluent : CH 3 OH/H2O = 80/20Flow Rate : 1.0mL/min15Col. Temp. : 40℃14131211109A : Zorbax Eclipse plusB : Phenomenex Luna C18(2)C : Hypersil GOLD C18D : SunfireE : Symmetry C18F : Atlantis T3G : YMC Pro C18Verylowlevel876www.winlab.<strong>com</strong>.<strong>au</strong> Ph 61 07 3205 5233<strong>Inertsil</strong><strong>ODS</strong>-3A B C D E F GFax 61 07 3205 1209 email: info@winlab.<strong>com</strong>.<strong>au</strong>
Durability of <strong>Inertsil</strong>Durability Test:Introducing 500 volumes (times) of0.1% TFA against the column volume.Upper Chromatogram: BeforeLower Chromatogram: AfterPhenolPyridine0 2 4 6 8 10 12 14 16Time (min)Retention Time Volatility (%)100908070600Comparison ofDurability Test with othermanufacturer’s column<strong>Inertsil</strong> <strong>ODS</strong>-3100 200 300 400 500 600Immersed time of TFA (hrs)Column AColumn BColumn CColumn DColumn EColumn FColumn G0 2 4 6 8 10 12 14 16Time (min)18www.winlab.<strong>com</strong>.<strong>au</strong> Ph 61 07 3205 5233Fax 61 07 3205 1209 email: info@winlab.<strong>com</strong>.<strong>au</strong>
®<strong>Inertsil</strong> <strong>ODS</strong>-4 is a C18 column developed focusing on superbinertness to basic, acidic, neutral and chelating <strong>com</strong>pounds, whichcan virtually provide perfect peak shapes for any type of <strong>com</strong>pounds.Physical PropertiesSilicaPurityParticle SizeSurface AreaPore VolumePore SizeBonded PhaseEnd-cappingCarbon LoadingUSP Codewww.winlab.<strong>com</strong>.<strong>au</strong> Ph 61 07 3205 5233: High Purity Spherical Silica Gel: 99.999%: 5um: 450 m2/g: 100A: 1.05 mL/g: Octadecyl groups: Yes: 11%: L1Fax 61 07 3205 1209 email: info@winlab.<strong>com</strong>.<strong>au</strong>19
Adsorption Performance1~ Basic Compounds ~1) Pyridine2) Phenol20 2 4 6 8 10 12Time (min)<strong>Inertsil</strong> <strong>ODS</strong>-412www.winlab.<strong>com</strong>.<strong>au</strong> Ph 61 07 3205 5233Fax 61 07 3205 1209 email: info@winlab.<strong>com</strong>.<strong>au</strong>0 2 4 6 8 10 12Time (min)Zorbax Eclipse plus C1820
Adsorption Performance- Strong Basic Compounds -DextromethorphanN = 9,600Assy. = 1.500 2 4 6 8 10Time (min)<strong>Inertsil</strong> <strong>ODS</strong>-4N = 1,500Assy. = 8.99www.winlab.<strong>com</strong>.<strong>au</strong> Ph 61 07 3205 5233Fax 61 07 3205 1209 email: info@winlab.<strong>com</strong>.<strong>au</strong>0 2 4 6 8 10 12Time (min)Zorbax Eclipse plus C1821
Adsorption Performance- Strong Chelating Compounds -β- Thujaplicin (Hinokitiol)It is ideal to have a peakshape that provides a highquantitative peak shapefrom the 1 st injection.0 2 4 6 8 10 12Time (min)<strong>Inertsil</strong> <strong>ODS</strong>-40 2 4 6 8 10 12Time (min)www.winlab.<strong>com</strong>.<strong>au</strong> Ph 61 07 3205 5233Fax 61 07 3205 1209 email: info@winlab.<strong>com</strong>.<strong>au</strong>Zorbax Eclipse plus C1822
Features of <strong>Inertsil</strong> <strong>ODS</strong>-4* Perfect End-capping techniqueA newly developed state-of-the-art end-capping technique ideallydeactivates the residual silanol groups, resulting in preventing adsorptionof highly basic <strong>com</strong>pounds. In addition, since the surface of the packingmaterial is neutral, adsorption of neutral and highly acidic <strong>com</strong>poundsdoes not occur.* Intensive elimination of trace metals on the silica gelsurfaceWhen analyzing chelating <strong>com</strong>pounds, it can be analyzed without anymasking operations to those metals that are exposed on the surface ofthe packing material.* Extreme durability to acidic mobile phase conditions* High stability to 100% aqueous mobile phaseconditions100% aqua is used with the proper chemical bonded.23
Ordering InformationAnalytical / Preparative ColumnID (mm) 1.0 1.5 2.1 3.0 4.0 4.6Length (mm) Cat.No Cat.No Cat.No Cat.No Cat.No Cat.No30 5020-81011 5020-81021 5020-03911 5020-03921 5020-03931 5020-0394150 5020-81012 5020-81022 5020-03912 5020-03922 5020-03932 5020-0394275 5020-81013 5020-81023 5020-03913 5020-03923 5020-03933 5020-03943100 5020-81014 5020-81024 5020-03914 5020-03924 5020-03934 5020-03944150 5020-81015 5020-81025 5020-03915 5020-03925 5020-03935 5020-03945250 5020-81016 5020-81026 5020-03916 5020-03926 5020-03936 5020-03946ID (mm) 6.0 7.6 10.0 20.0Length (mm) Cat.No Cat.No Cat.No Cat.NoGuard 50 5020-03957 5020-03967 5020-81057 5020-81067100 5020-03954 5020-03964 5020-81054 5020-81064150 5020-03955 5020-03965 5020-81055 5020-81065250 5020-03956 5020-03966 5020-81056 5020-81066* End-fitting is Waters 1/16” type.* For other column sizes, please send us an enquiry. (Upon request)We released2um of <strong>Inertsil</strong> <strong>ODS</strong>-4www.winlab.<strong>com</strong>.<strong>au</strong> Ph 61 07 3205 5233Fax 61 07 3205 1209 email: info@winlab.<strong>com</strong>.<strong>au</strong>24
The First choice <strong>ODS</strong> columns is...Please re<strong>com</strong>mend an <strong>ODS</strong> column that will show aseparation behavior based on hydrophobic interaction,which will be a … Monomeric type <strong>ODS</strong>Excellent planar molecular recognition, a Polymeric type <strong>ODS</strong> (<strong>Inertsil</strong><strong>ODS</strong>-P) or excellent selectivity for polar <strong>com</strong>pounds.GL Sciences’ Re<strong>com</strong>mended First Choice Column- <strong>Inertsil</strong> <strong>ODS</strong>-4or- <strong>Inertsil</strong> <strong>ODS</strong>-325www.winlab.<strong>com</strong>.<strong>au</strong> Ph 61 07 3205 5233Fax 61 07 3205 1209 email: info@winlab.<strong>com</strong>.<strong>au</strong>
The First Choice <strong>ODS</strong> columnWhen and How to re<strong>com</strong>mend <strong>Inertsil</strong> <strong>ODS</strong>-4* Simultaneous analysis of Pesticides or AntibacterialsStrong adsorptive <strong>com</strong>pounds may be included in these samples.In this case, <strong>Inertsil</strong> <strong>ODS</strong>-4 will be the best column to re<strong>com</strong>mend.* Analyzing trace amount of samples using LC/MS(/MS)If the targeted <strong>com</strong>pounds c<strong>au</strong>ses even those slight adsorptions, the precisionof quantification will decrease. Therefore, please re<strong>com</strong>mend <strong>Inertsil</strong> <strong>ODS</strong>-4.* Unsatisfied with the peak shape that the current column is producingSome type of <strong>com</strong>pounds are probably being adsorbed to the packing material.Please re<strong>com</strong>mend <strong>Inertsil</strong> <strong>ODS</strong>-4. as it provides extremely low adsorption.* Analysis of 100% aqueous mobile phase in gradient modeSince high carbon loading columns doesn’t blend well with water, it takes timeto equilibrate the column to the initial mobile phase.Please re<strong>com</strong>mend <strong>Inertsil</strong> <strong>ODS</strong>-4 as the equilibration time is more less.www.winlab.<strong>com</strong>.<strong>au</strong> Ph 61 07 3205 5233Fax 61 07 3205 1209 email: info@winlab.<strong>com</strong>.<strong>au</strong>26
The First Choice <strong>ODS</strong> columnWhen and How to re<strong>com</strong>mend other <strong>Inertsil</strong> columns* Mutual separation of isomers (cis/trans, o-/m-/p-, n-/iso-)* Simultaneous analysis of PAH (Poly Aromatic Hydrocarbons)Please re<strong>com</strong>mend <strong>Inertsil</strong> <strong>ODS</strong>-P as it has excellent planar molecularrecognition.* Analysis of highly hydrophobic <strong>com</strong>poundsPlease re<strong>com</strong>mend <strong>Inertsil</strong> <strong>ODS</strong>-SP as it has low carbon loading.Or re<strong>com</strong>mending <strong>Inertsil</strong> C8-3 as it has short alkyl chains.* Analysis of highly hydrophobic contaminants (Lipids, Natural Colorants)Column deterioration can be generated by the accumulation of highlyhydrophobic <strong>com</strong>pounds to the packing material.In this case, please re<strong>com</strong>mend <strong>Inertsil</strong> <strong>ODS</strong>-SP as it has low carbon loading.* Analysis of MetabolitesWhen finding difficulty separating those metabolites using a C18 column,please try <strong>Inertsil</strong> Ph-3. Many pharmaceutical researchers are finding<strong>Inertsil</strong> Ph-3 useful for the analysis of metabolites.www.winlab.<strong>com</strong>.<strong>au</strong> Ph 61 07 3205 5233Fax 61 07 3205 1209 email: info@winlab.<strong>com</strong>.<strong>au</strong>27
If you have any questions about <strong>Inertsil</strong> columns,please do not hesitate to contact us.E-mail: info@winlab.<strong>com</strong>.<strong>au</strong>PH 61 7 3205 5233 FX 61 7 3205 120928
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