mosquito LCP® - Hampton Research

mosquito LCP ®A fast and fully automated solutionfor Lipidic Cubic Phase screeningJoby Jenkins, Product Manager

mosquito LCP: Key Elementsspool of disposablemosquito pipette tipsLCP syringedispensing pumpmosquito pipetting headLCP syringe2 or 4 position plateX/Y stage

mosquito: Pipette Tips• volume range: 25 – 1,200nL• disposable: zero cross-contamination• true positive displacement• accurate for solvents and viscous liquids• negligible dead volume• cannot get blocked• ability to mix• ability to pierce foil plate seals• multi-aspirate/multi-dispense capabilityPolyesterbacking tapeStainlesssteel pistonHigh densitypolyethylenebarrel

mosquito LCP: Syringe Dispenser• syringe types: 50µL or 100µL• dispense range: 25nL – 2µL• true positive displacement• accurate for highly viscous LCP material• low dead volume• precise drop positioning• simple syringe changing mechanism• well proven LCP dispensing technologyHigh precisionsyringe pump driverGas tight positivedisplacement syringeDispense needle

mosquito LCP: Features• Developed in collaboration with The MRC,Cambridge, UK• Enables the automated set-up of In mesocrystallisation experiments• Compact bench top instrument• Positive displacement syringe foraccurate, low volume LCP dispense• Auto calibrating syringe needle for highlyaccurate LCP drop placement• Disposable, positive displacementmosquito tips for accurate screen addition• High throughput,

mosquito LCP: Auto-Calibration• LCP needle tip position isautomatically measured and calibrated• High precision photoelectric sensorsdetect position in X, Y and Z axes• Positioning to within ± 5 µm isachieved, resulting in:‣ Precise placement of LCP drops‣ Accurate overlay of screensolutions‣ Known drop position forrepeatable automated imaging• Facilitates quick and easy syringechanging with no manual recalibration

mosquito LCP: Drop Set-upmosquito LCP syringe pipetting 100nL of highly viscous LCP sample on to an LCP Sandwich Plate• High precision Z axis control for accurate contact dispensing and sample ‘touch of’• Automatic Z height dispense offsets throughout LCP dispense volume range

mosquito LCP: Drop Set-upmosquito tips pipetting 800nL of screen buffers directly on to LCP drops on an LCP Sandwich Plate• Screen solutions are quickly pipetted on to LCP drops, minimising exposure time• High precision Z axis control ensures LCP drops are not touched by the pipette tips• Precise X/Y positioning ensures screen buffer drops surround the LCP drops

mosquito LCP: CrystalsLCP drop imaged in Sandwich PlateHigh magnification LCP drop with crystalsImage supplied by Pat Edwards/Tony Warne, MRC Cambridge, UK

mosquito LCP: CrystalsLCP drops with crystals imaged in Sandwich PlateImages supplied by Jim Fairman/Susan Buchanan, NIDDK, NIH, USA

mosquito LCP: CrystalsLCP drops with crystals imaged in Sandwich PlateImages supplied by Jim Fairman/Susan Buchanan, NIDDK, NIH, USA

mosquito LCP: CrystalsLCP Hanging DropHigh magnification LCP drop with crystalsImage supplied by Reinhard Grisshammer, NINDS, NIH, USA

mosquito LCP: Accessories

mosquito LCP: Key Benefits• specifically designed for automated, low volume, LCP screening• based on mosquito – extremely reliable and very low maintenance– proven in many, many, crystallography labs around the world• simple and robust technology• positive displacement, disposable tips for screen buffer dispensing• volumetrically precise and accurate throughout volume and viscosity ranges• highly accurate drop placement• self calibrating LCP syringe• compact bench-top instrument• versatile; Hanging Drop, Sitting Drop & Microbatch and LCP set ups• multi-aspirate and mixing options give added flexibility (seeding, additives etc)• very easy to use, user friendly software and hardware

mosquito LCP ®A fast and fully automated solutionfor Lipidic Cubic Phase screeningJoby Jenkins, Product Manager