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<strong>mosquito</strong> LCP ®A fast and fully automated solutionfor Lipidic Cubic Phase screeningJoby Jenkins, Product Manager
<strong>mosquito</strong> LCP: Key Elementsspool of disposable<strong>mosquito</strong> pipette tipsLCP syringedispensing pump<strong>mosquito</strong> pipetting headLCP syringe2 or 4 position plateX/Y stage
<strong>mosquito</strong>: Pipette Tips• volume range: 25 – 1,200nL• disposable: zero cross-contamination• true positive displacement• accurate for solvents and viscous liquids• negligible dead volume• cannot get blocked• ability to mix• ability to pierce foil plate seals• multi-aspirate/multi-dispense capabilityPolyesterbacking tapeStainlesssteel pistonHigh densitypolyethylenebarrel
<strong>mosquito</strong> LCP: Syringe Dispenser• syringe types: 50µL or 100µL• dispense range: 25nL – 2µL• true positive displacement• accurate for highly viscous LCP material• low dead volume• precise drop positioning• simple syringe changing mechanism• well proven LCP dispensing technologyHigh precisionsyringe pump driverGas tight positivedisplacement syringeDispense needle
<strong>mosquito</strong> LCP: Features• Developed in collaboration with The MRC,Cambridge, UK• Enables the automated set-up of In mesocrystallisation experiments• Compact bench top instrument• Positive displacement syringe foraccurate, low volume LCP dispense• Auto calibrating syringe needle for highlyaccurate LCP drop placement• Disposable, positive displacement<strong>mosquito</strong> tips for accurate screen addition• High throughput,
<strong>mosquito</strong> LCP: Auto-Calibration• LCP needle tip position isautomatically measured and calibrated• High precision photoelectric sensorsdetect position in X, Y and Z axes• Positioning to within ± 5 µm isachieved, resulting in:‣ Precise placement of LCP drops‣ Accurate overlay of screensolutions‣ Known drop position forrepeatable automated imaging• Facilitates quick and easy syringechanging with no manual recalibration
<strong>mosquito</strong> LCP: Drop Set-up<strong>mosquito</strong> LCP syringe pipetting 100nL of highly viscous LCP sample on to an LCP Sandwich Plate• High precision Z axis control for accurate contact dispensing and sample ‘touch of’• Automatic Z height dispense offsets throughout LCP dispense volume range
<strong>mosquito</strong> LCP: Drop Set-up<strong>mosquito</strong> tips pipetting 800nL of screen buffers directly on to LCP drops on an LCP Sandwich Plate• Screen solutions are quickly pipetted on to LCP drops, minimising exposure time• High precision Z axis control ensures LCP drops are not touched by the pipette tips• Precise X/Y positioning ensures screen buffer drops surround the LCP drops
<strong>mosquito</strong> LCP: CrystalsLCP drop imaged in Sandwich PlateHigh magnification LCP drop with crystalsImage supplied by Pat Edwards/Tony Warne, MRC Cambridge, UK
<strong>mosquito</strong> LCP: CrystalsLCP drops with crystals imaged in Sandwich PlateImages supplied by Jim Fairman/Susan Buchanan, NIDDK, NIH, USA
<strong>mosquito</strong> LCP: CrystalsLCP drops with crystals imaged in Sandwich PlateImages supplied by Jim Fairman/Susan Buchanan, NIDDK, NIH, USA
<strong>mosquito</strong> LCP: CrystalsLCP Hanging DropHigh magnification LCP drop with crystalsImage supplied by Reinhard Grisshammer, NINDS, NIH, USA
<strong>mosquito</strong> LCP: Accessories
<strong>mosquito</strong> LCP: Key Benefits• specifically designed for automated, low volume, LCP screening• based on <strong>mosquito</strong> – extremely reliable and very low maintenance– proven in many, many, crystallography labs around the world• simple and robust technology• positive displacement, disposable tips for screen buffer dispensing• volumetrically precise and accurate throughout volume and viscosity ranges• highly accurate drop placement• self calibrating LCP syringe• compact bench-top instrument• versatile; Hanging Drop, Sitting Drop & Microbatch and LCP set ups• multi-aspirate and mixing options give added flexibility (seeding, additives etc)• very easy to use, user friendly software and hardware
<strong>mosquito</strong> LCP ®A fast and fully automated solutionfor Lipidic Cubic Phase screeningJoby Jenkins, Product Manager