Izit User Guide - Hampton Research

Izit Crystal DyeSolutions for Crystal GrowthUser Guide HR4-710 (pg 1)ApplicationDifferentiate protein crystals from salt crystals.Figure 1Protein crystals before Izit (left) and after Izit (right)Features• 0.22 micron sterile filtered solution• Small molecule dye penetrates solvent channels ofmacromolecular crystals, coloring the crystals blue.• Salt crystals do not absorb the dye and remain colorlessDescriptionIzit is a small molecule dye designed to differentiate biological macromoleculecrystals from small molecule and inorganic crystals. 1-2 Biological macromoleculecrystals contain large solvent channels allowing Izit to penetrateand color the crystal blue. Small molecule and inorganic crystals do notpossess large solvent channels and cannot absorb Izit; hence they will nottake up the blue color.Application• To an existing drop with crystal(s) to differentiate salt from protein:Place a small amount of Izit into the drop containing the crystal(s). Example;pipette 1 ml of Izit into a 10 microliter drop or 0.5 ml into a 4 microliterdrop. Izit does not need to be added to the reservoir.For smaller drops (

Izit Crystal DyeUser Guide HR4-710 (pg 2)3. Crystallization and preliminary X-ray analysis of Alicyclobacillus acidocaldariusendoglucanase CelA. K. Eckert, H. A. Ernst, E. Schneider, S. Larsenand Lo Leggio. Acta Cryst. (2003). D59, 139-141.4. Searching for silver bullets: An alternative strategy for crystallizing macromolecules.Alexander McPherson and Bob Cudney. Journal of StructuralBiology 156 (2006) 387-406.Technical SupportInquiries regarding Izit and general inquiries regarding crystallization arewelcome. Please email, fax, or telephone your request to Hampton Research.Fax and e-mail Technical Support are available 24 hours a day. Telephonetechnical support is available 8:00 a.m. to 5:00 p.m. USA Pacific StandardTime.Solutions for Crystal Growth