Patterned and switchable surfaces for biomaterial applications

Andrew Hook – Patterned and switchable surfaces for biomaterial applications(A)(B)(C)(D)(E) (F) (G)Figure 2.6. Fluorescence microscopy images of 6-FAM labelled oligonucleotideadsorption on a patterned ALAPP-PEG surface detected through a 450-490nm excitation filter and a 515 nm suppression filter. Images A) - D) and E) -G) represent two separate experiments. A) 10 l of 290 ng/loligonucleotide solution injected over patterned surface and incubated for 5min. B) +2 V applied to substrate and incubated for 5 min. C) Surfacewashed with 100 l ultra pure water whilst still applying +2 V. D) Surfacewashed whilst 100 l ultra pure water whilst applying –2 V. E) 10 l of 290ng/l oligonucleotide solution injected over patterned surface and incubatedfor 5 min. F) +1.5 V applied to substrate and incubated for 5 min. G) -1.5 Vapplied to substrate and incubated for 5 min. Images A) and B) haveidentical exposure times. Images C) and D) have identical exposure times.Images E), F) and G) have identical exposure times. The scale bar equals100 m.2-85