Patterned and switchable surfaces for biomaterial applications

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Chapter 2 – Spatially controlled electro-stimulated DNA adsorption and desorption for biodeviceapplicationsindicating that a small amount of DNA remains adsorbed to the surface despite theapplication of a negative bias (-1.5 V).2-84
Andrew Hook – Patterned and switchable surfaces for biomaterial applications(A)(B)(C)(D)(E) (F) (G)Figure 2.6. Fluorescence microscopy images of 6-FAM labelled oligonucleotideadsorption on a patterned ALAPP-PEG surface detected through a 450-490nm excitation filter and a 515 nm suppression filter. Images A) - D) and E) -G) represent two separate experiments. A) 10 l of 290 ng/loligonucleotide solution injected over patterned surface and incubated for 5min. B) +2 V applied to substrate and incubated for 5 min. C) Surfacewashed with 100 l ultra pure water whilst still applying +2 V. D) Surfacewashed whilst 100 l ultra pure water whilst applying –2 V. E) 10 l of 290ng/l oligonucleotide solution injected over patterned surface and incubatedfor 5 min. F) +1.5 V applied to substrate and incubated for 5 min. G) -1.5 Vapplied to substrate and incubated for 5 min. Images A) and B) haveidentical exposure times. Images C) and D) have identical exposure times.Images E), F) and G) have identical exposure times. The scale bar equals100 m.2-85
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Patterned andswitchable surfacesfor
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TABLE OF CONTENTSTABLE OF CONTENTS
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Andrew Hook - Patterned and switcha
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CHAPTER 1.INTRODUCTION1The content
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Chapter 1 - Introductionconsiderabl
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Chapter 1 - Introductioninteraction
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Chapter 1 - IntroductionIn general,
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Chapter 1 - IntroductionFor some ap
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Chapter 1 - Introductionangles rang
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Chapter 1 - Introductionof understa
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Chapter 1 - IntroductionSeveral str
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Chapter 1 - Introductioncues to con
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Chapter 1 - Introductionby controll
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Chapter 1 - Introduction1.2.2. Soft
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Chapter 1 - Introductionsubstrate.
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Chapter 1 - Introduction(A)(B)Figur
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Chapter 1 - Introduction(A)(B)(C)(C
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Chapter 1 - Introductioncomplex gen
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Chapter 1 - Introductionmorphology
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Chapter 4 - Formation of a chemical
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CHAPTER 5.SURFACE PLASMON RESONANCE
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SPR signal intensity (counts)Andrew
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Reflectivity (%) Reflectivity (%)An
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Reflectivity (%)SPR signal intensit
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SPR signal intensity (counts)Andrew
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Thickness (nm)Thickness (nm)Thickne
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CHAPTER 6.OVERALL CONCLUSIONS6.6An
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Chapter 6 - Overall conclusionsThes
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Chapter 6 - Overall conclusionsnew
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Force (nN)Force (nN)Force (nN)Force
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Patterned and switchable surfaces for biomaterial applications

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