Patterned and switchable surfaces for biomaterial applications

Andrew Hook – Patterned and switchable surfaces for biomaterial applicationsexpressing pEGFP-N1 and the total cell population was calculated and thetransfection efficiency was determined by dividing the number of transfected cellsby the total number of cells.2.3. Results and discussion2.3.1. Characterisation of polymer filmsChemical characterisation of untreated and surface modified p ++ Si wafers wascarried out by XPS analysis. The elemental composition of each surface wasdetermined from survey spectra (Table 2.1). High-resolution XPS spectra of the C 1speak are shown in Figure 2.4. Both C 1s spectra could be de-convoluted into fourcomponents. In order of ascending binding energy, the four peaks correspond toaliphatic (C-H and C-C) carbon at 285.0 eV, C-O and C-N groups at 286.5 eV,amide and carbonyl groups at 288.0 eV, and ester and carboxylic acid groups at289.0 eV. The quantification of the C 1s high-resolution spectra is summarised inTable 2.2.2-75