Patterned and switchable surfaces for biomaterial applications

More documents

Recommendations

Info

Chapter 2 – Spatially controlled electro-stimulated DNA adsorption and desorption for biodeviceapplicationsFigure 2.1. Schematic of the formation of a TCM. Silicon was modified sequentiallyby plasma polymerisation and PEG grafting. Laser ablation was used toform ALAPP wells, a robotic arrayer was then used to spot DNA into thesewells. Cells seeded onto this surface grew exclusively within the wells, on topof the DNA spots. Application of a negative voltage triggered the release ofDNA from the surface, making it available for uptake by nearby, adheredcells. Transfected cells are depicted as green. Schematic not drawn to scale.2-66
Andrew Hook – Patterned and switchable surfaces for biomaterial applicationsThe adsorption and desorption of oligonucleotides and plasmid DNA to ALAPPwas manipulated by the application of an appropriate DC voltage to the semiconductingsilicon substrate in order to study the spatially controlled electrostimulatedmanipulation of DNA. Furthermore, the subsequent growth andtransfection of mammalian cells was conducted as depicted in Figure 2.1. Here, theenhancement of transfection efficiency by electro-stimulated DNA desorption was ofparticular interest.The unique combination of patterned and switchable components of this deviceallows unprecedented control over the behaviour of cells and DNA in fourdimensions. This approach is useful for overcoming the current limitations of TCMsand is promising for broader applications in advanced biodevices.2.2. Materials and methods2.2.1. Substrate preparationBoron doped p ++ silicon wafers (Virginia Semiconductors, Inc.) were cut intoapproximately 10 x 10 mm 2 pieces, cleaned by sonication for 30 min in a 5%surfactant (RBS 35, Pierce USA) solution and oxidised under UV light for 30 min.2.2.2. Plasma polymerisationPlasma polymerisation reactions were preformed in a custom-built reactordescribed elsewhere [182]. In short, the plasma reactor consisted of two circularelectrodes separated by 125 mm in a cylindrical reactor being 350 mm high with adiameter of 170 mm. Allylamine (Aldrich, 98% purity) was used as a monomer.2-67
Page 1 and 2:
Patterned andswitchable surfacesfor
Page 3 and 4:
TABLE OF CONTENTSTABLE OF CONTENTS
Page 5 and 6:
Andrew Hook - Patterned and switcha
Page 7 and 8:
Page 9 and 10:
Page 12 and 13:
CHAPTER 1.INTRODUCTION1The content
Page 14 and 15:
Chapter 1 - Introductionconsiderabl
Page 16 and 17:
Chapter 1 - Introductioninteraction
Page 18 and 19:
Chapter 1 - IntroductionIn general,
Page 20 and 21:
Chapter 1 - IntroductionFor some ap
Page 22 and 23:
Chapter 1 - Introductionangles rang
Page 24 and 25:
Chapter 1 - Introductionof understa
Page 26 and 27: Chapter 1 - IntroductionSeveral str
Page 28 and 29: Chapter 1 - Introductioncues to con
Page 30 and 31: Chapter 1 - Introductionby controll
Page 32 and 33: Chapter 1 - Introduction1.2.2. Soft
Page 34 and 35: Chapter 1 - Introductionsubstrate.
Page 36 and 37: Chapter 1 - Introduction(A)(B)Figur
Page 38 and 39: Chapter 1 - Introduction(A)(B)(C)(C
Page 40 and 41: Chapter 1 - Introductioncomplex gen
Page 42 and 43: Chapter 1 - Introductionmorphology
Page 44 and 45: Chapter 1 - Introduction(A)(B)(C)(D
Page 46 and 47: Chapter 1 - Introductionthe LCST, a
Page 48 and 49: Chapter 1 - IntroductionHyun et al.
Page 50 and 51: Chapter 1 - Introductionmicelles. T
Page 52 and 53: Chapter 1 - Introduction1.4.1. DNA
Page 54 and 55: Chapter 1 - IntroductionA major pro
Page 56 and 57: Chapter 1 - Introductionattached to
Page 58 and 59: Chapter 1 - IntroductionTable 1.1.
Page 60 and 61: Chapter 1 - Introductionefficiencie
Page 62 and 63: Chapter 1 - Introductionachieved by
Page 64 and 65: Chapter 1 - IntroductionTable 1.2.
Page 66 and 67: Chapter 1 - IntroductionOnce releas
Page 68 and 69: Chapter 1 - Introductionimaging not
Page 70 and 71: Chapter 1 - IntroductionA number of
Page 73 and 74: CHAPTER 2.SPATIALLY CONTROLLED ELEC
Page 75: Andrew Hook - Patterned and switcha
Page 79 and 80: Andrew Hook - Patterned and switcha
Page 107 and 108: CHAPTER 3.COMPARISON OF THE BINDING
Page 123 and 124: DNA (mg/m 2 ) DNA (mg/m 2 )Andrew H
Page 127 and 128:
Page 129 and 130:
Page 131 and 132:
DNA (mg/m 2 )Andrew Hook - Patterne
Page 133 and 134:
Page 135 and 136:
Page 137:
Page 140 and 141:
Chapter 4 - Formation of a chemical
Page 142 and 143:
Page 144 and 145:
Page 146 and 147:
Page 148 and 149:
Page 150 and 151:
Page 152 and 153:
Page 154 and 155:
Page 156 and 157:
Page 158 and 159:
Page 160 and 161:
Page 162 and 163:
Page 165 and 166:
CHAPTER 5.SURFACE PLASMON RESONANCE
Page 167 and 168:
Page 169 and 170:
Page 171 and 172:
Page 173 and 174:
Page 175 and 176:
Page 177 and 178:
Page 179 and 180:
Page 181 and 182:
SPR signal intensity (counts)Andrew
Page 183 and 184:
Page 185 and 186:
Page 187 and 188:
Page 189 and 190:
Page 191 and 192:
Page 193 and 194:
Reflectivity (%) Reflectivity (%)An
Page 195 and 196:
Reflectivity (%)SPR signal intensit
Page 197 and 198:
SPR signal intensity (counts)Andrew
Page 199 and 200:
Page 201 and 202:
Page 203 and 204:
Page 205 and 206:
Thickness (nm)Thickness (nm)Thickne
Page 207 and 208:
Page 209 and 210:
Page 211 and 212:
Page 213 and 214:
Page 215 and 216:
Page 218 and 219:
CHAPTER 6.OVERALL CONCLUSIONS6.6An
Page 220 and 221:
Chapter 6 - Overall conclusionsThes
Page 222 and 223:
Chapter 6 - Overall conclusionsnew
Page 224 and 225:
Page 226 and 227:
Page 228 and 229:
Page 230 and 231:
Page 232 and 233:
Force (nN)Force (nN)Force (nN)Force
Page 234 and 235:
Force (nN)Force (nN)Andrew Hook - P
Page 236 and 237:
Page 238 and 239:
Page 240 and 241:
Page 242 and 243:
show all

Patterned and switchable surfaces for biomaterial applications

Create successful ePaper yourself

Delete template?

Save as template?