Patterned and switchable surfaces for biomaterial applications
Patterned and switchable surfaces for biomaterial applications
Patterned and switchable surfaces for biomaterial applications
You also want an ePaper? Increase the reach of your titles
YUMPU automatically turns print PDFs into web optimized ePapers that Google loves.
Andrew Hook – <strong>Patterned</strong> <strong>and</strong> <strong>switchable</strong> <strong>surfaces</strong> <strong>for</strong> <strong>biomaterial</strong> <strong>applications</strong>variety of gene functions <strong>and</strong> cell lines. Furthermore, the development of cDNA <strong>and</strong>RNAi genome wide libraries would enable the high-throughput, rapid <strong>and</strong>inexpensive analysis of entire genomes, with each gene either overexpressed orsilenced at defined cell clusters, all upon a single glass slide.However, a number of key issues must be resolved be<strong>for</strong>e TCMs can become thehigh-throughput tool that is desired. Methods to manage low transfection efficiencies<strong>and</strong> effective colony separation needs to be developed. Recent developmentsdiscussed in this chapter point to a solution of these problems. The greatest<strong>for</strong>eseeable challenge is the ability to process tens of thous<strong>and</strong>s of colonies, allpotentially with a varied phenotype. A number of automated fluorescence systemsare being developed, <strong>and</strong> further progress in this area is essential <strong>for</strong> the successfulimplementation of TCMs. The use of fluorescence tags <strong>and</strong> GFP as a reporter geneare pivotal to this analysis <strong>and</strong> have been used successfully <strong>for</strong> a number of genefunction studies. However, the further development of other screening methods thatinfer gene function would broaden the scope of TCM <strong>applications</strong> to studyphenotypes where fluorescence tagging or staining is not viable.1-61
Change language