Patterned and switchable surfaces for biomaterial applications

More documents

Recommendations

Info

Andrew Hook – Patterned and switchable surfaces for biomaterial applications2.2.1. Substrate preparation....................................................................................... 2-672.2.2. Plasma polymerisation ..................................................................................... 2-672.2.3. Poly(ethylene glycol) grafting .......................................................................... 2-682.2.4. Excimer laser ablation ..................................................................................... 2-682.2.5. X-ray photoelectron spectroscopy..................................................................... 2-702.2.6. Fourier transform – infrared analysis ............................................................... 2-702.2.7. Plasmid propagation ........................................................................................ 2-702.2.8. DNA adsorption and desorption studies ............................................................ 2-712.2.9. Solid phase transfection ................................................................................... 2-732.3. RESULTS AND DISCUSSION ..................................................................................... 2-752.3.1. Characterisation of polymer films .................................................................... 2-752.3.2. DNA adsorption and desorption studies ............................................................ 2-822.3.3. Transfection experiments.................................................................................. 2-892.3.4. Formation of a transfected cell microarray....................................................... 2-942.4. CONCLUSION ........................................................................................................ 2-95CHAPTER 3. COMPARISON OF THE BINDING MODE OF PLASMID DNA TOALLYLAMINE PLASMA POLYMER AND POLY(ETHYLENE GLYCOL) SURFACES . 3-973.1. INTRODUCTION ..................................................................................................... 3-973.2. MATERIALS AND METHODS ...................................................................................3-1013.2.1. Substrate preparations ....................................................................................3-1013.2.2. Substrate characterisation ...............................................................................3-1023.2.3. Plasmid preparation .......................................................................................3-1033.2.4. DNA adsorption ..............................................................................................3-1043.2.5. Data analysis ..................................................................................................3-1053.2.6. Electrostimulated DNA adsorption and desorption ..........................................3-1063.3. RESULTS AND DISCUSSION ....................................................................................3-1083.3.1. Surface characterisation .................................................................................3-1083.3.2. Comparison of in situ QCM analysis and depletion of solution fluorescence ....3-1113.3.3. DNA adsorption. .............................................................................................3-1143.3.4. Electro-stimulated DNA adsorption and desorption .........................................3-1253.4. CONCLUSION .......................................................................................................3-127CHAPTER 4. ADVANCED SUBSTRATE FABRICATION FOR CELLMICROARRAYS ................................................................................................ 4-1294.1. INTRODUCTION ....................................................................................................4-1294.2. MATERIALS AND METHODS ...................................................................................4-1344.2.1. Substrate preparation......................................................................................4-1344.2.2. Array formation ..............................................................................................4-1344.2.3. Characterisation of polymer crosslinking ........................................................4-1374.2.4. Cell culture .....................................................................................................4-137II
Andrew Hook – Patterned and switchable surfaces for biomaterial applications4.3. RESULTS AND DISCUSSION ....................................................................................4-1384.3.1. Optimisation of polymer printing .....................................................................4-1384.3.2. Polymer microarray formation ........................................................................4-1394.3.3. Polymer microarray characterisation ..............................................................4-1434.3.4. Cell microarray formation...............................................................................4-1484.4. CONCLUSION .......................................................................................................4-152CHAPTER 5. SURFACE PLASMON RESONANCE IMAGING OF POLYMERMICROARRAYS ................................................................................................ 5-1555.1. INTRODUCTION ....................................................................................................5-1555.2. THEORY BEHIND SURFACE PLASMON RESONANCE IMAGING ....................................5-1585.3. MATERIALS AND METHODS ...................................................................................5-1625.3.1. Substrate preparation......................................................................................5-1625.3.2. Polymerisation of poly(N-isopropylacrylamide) ...............................................5-1635.3.3. Array formation ..............................................................................................5-1635.3.4. SPR imaging ...................................................................................................5-1655.3.5. Study of switching of PNIPAAm by SPR imaging .............................................5-1665.3.6. Cell growth .....................................................................................................5-1675.3.7. Kinetic analysis ...............................................................................................5-1675.4. RESULTS AND DISCUSSION ....................................................................................5-1695.4.1. SPR imaging of a PNIPAAm microarray .........................................................5-1695.4.2. Standardising SPR signal on a polymer microarray .........................................5-1745.4.3. Kinetic analysis of protein adsorption to polymer array ...................................5-1885.4.4. Assessment of cell attachment to polymer arrays .............................................5-2035.5. CONCLUSION .......................................................................................................5-205CHAPTER 6. OVERALL CONCLUSIONS ...................................................... 6-208APPENDIX 1. LIST OF ABBREVIATIONS ...........................................................AAPPENDIX 2. AFM FORCE CURVES OF CROSS-LINKED POLYMER ARRAYS .CA2.1. INTRODUCTION .......................................................................................................... CA2.2. MATERIALS AND METHODS ......................................................................................... DA2.2.1. Plasma polymerisation .......................................................................................... DA2.2.2. Polymer array formation ....................................................................................... DA2.2.3. Polymer spin coating............................................................................................. EA2.2.4. AFM tip modification ............................................................................................ FA2.2.5. AFM force measurements ...................................................................................... FA2.3. RESULTS .................................................................................................................... GA2.3.1. Polymer spot characterisation ............................................................................... GBIBLIOGRAPHY ................................................................................................ MIII
Page 1 and 2: Patterned andswitchable surfacesfor
Page 3: TABLE OF CONTENTSTABLE OF CONTENTS
Page 7 and 8: Andrew Hook - Patterned and switcha
Page 9 and 10: Andrew Hook - Patterned and switcha
Page 12 and 13: CHAPTER 1.INTRODUCTION1The content
Page 14 and 15: Chapter 1 - Introductionconsiderabl
Page 16 and 17: Chapter 1 - Introductioninteraction
Page 18 and 19: Chapter 1 - IntroductionIn general,
Page 20 and 21: Chapter 1 - IntroductionFor some ap
Page 22 and 23: Chapter 1 - Introductionangles rang
Page 24 and 25: Chapter 1 - Introductionof understa
Page 26 and 27: Chapter 1 - IntroductionSeveral str
Page 28 and 29: Chapter 1 - Introductioncues to con
Page 30 and 31: Chapter 1 - Introductionby controll
Page 32 and 33: Chapter 1 - Introduction1.2.2. Soft
Page 34 and 35: Chapter 1 - Introductionsubstrate.
Page 36 and 37: Chapter 1 - Introduction(A)(B)Figur
Page 38 and 39: Chapter 1 - Introduction(A)(B)(C)(C
Page 40 and 41: Chapter 1 - Introductioncomplex gen
Page 42 and 43: Chapter 1 - Introductionmorphology
Page 44 and 45: Chapter 1 - Introduction(A)(B)(C)(D
Page 46 and 47: Chapter 1 - Introductionthe LCST, a
Page 48 and 49: Chapter 1 - IntroductionHyun et al.
Page 50 and 51: Chapter 1 - Introductionmicelles. T
Page 52 and 53: Chapter 1 - Introduction1.4.1. DNA
Page 54 and 55:
Chapter 1 - IntroductionA major pro
Page 56 and 57:
Chapter 1 - Introductionattached to
Page 58 and 59:
Chapter 1 - IntroductionTable 1.1.
Page 60 and 61:
Chapter 1 - Introductionefficiencie
Page 62 and 63:
Chapter 1 - Introductionachieved by
Page 64 and 65:
Chapter 1 - IntroductionTable 1.2.
Page 66 and 67:
Chapter 1 - IntroductionOnce releas
Page 68 and 69:
Chapter 1 - Introductionimaging not
Page 70 and 71:
Chapter 1 - IntroductionA number of
Page 73 and 74:
CHAPTER 2.SPATIALLY CONTROLLED ELEC
Page 75 and 76:
Andrew Hook - Patterned and switcha
Page 77 and 78:
Page 79 and 80:
Page 81 and 82:
Page 83 and 84:
Page 85 and 86:
Page 87 and 88:
Page 89 and 90:
Page 91 and 92:
Page 93 and 94:
Page 95 and 96:
Page 97 and 98:
Page 99 and 100:
Page 101 and 102:
Page 103 and 104:
Page 105 and 106:
Page 107 and 108:
CHAPTER 3.COMPARISON OF THE BINDING
Page 109 and 110:
Page 111 and 112:
Page 113 and 114:
Page 115 and 116:
Page 117 and 118:
Page 119 and 120:
Page 121 and 122:
Page 123 and 124:
DNA (mg/m 2 ) DNA (mg/m 2 )Andrew H
Page 125 and 126:
Page 127 and 128:
Page 129 and 130:
Page 131 and 132:
DNA (mg/m 2 )Andrew Hook - Patterne
Page 133 and 134:
Page 135 and 136:
Page 137:
Page 140 and 141:
Chapter 4 - Formation of a chemical
Page 142 and 143:
Page 144 and 145:
Page 146 and 147:
Page 148 and 149:
Page 150 and 151:
Page 152 and 153:
Page 154 and 155:
Page 156 and 157:
Page 158 and 159:
Page 160 and 161:
Page 162 and 163:
Page 165 and 166:
CHAPTER 5.SURFACE PLASMON RESONANCE
Page 167 and 168:
Page 169 and 170:
Page 171 and 172:
Page 173 and 174:
Page 175 and 176:
Page 177 and 178:
Page 179 and 180:
Page 181 and 182:
SPR signal intensity (counts)Andrew
Page 183 and 184:
Page 185 and 186:
Page 187 and 188:
Page 189 and 190:
Page 191 and 192:
Page 193 and 194:
Reflectivity (%) Reflectivity (%)An
Page 195 and 196:
Reflectivity (%)SPR signal intensit
Page 197 and 198:
SPR signal intensity (counts)Andrew
Page 199 and 200:
Page 201 and 202:
Page 203 and 204:
Page 205 and 206:
Thickness (nm)Thickness (nm)Thickne
Page 207 and 208:
Page 209 and 210:
Page 211 and 212:
Page 213 and 214:
Page 215 and 216:
Page 218 and 219:
CHAPTER 6.OVERALL CONCLUSIONS6.6An
Page 220 and 221:
Chapter 6 - Overall conclusionsThes
Page 222 and 223:
Chapter 6 - Overall conclusionsnew
Page 224 and 225:
Page 226 and 227:
Page 228 and 229:
Page 230 and 231:
Page 232 and 233:
Force (nN)Force (nN)Force (nN)Force
Page 234 and 235:
Force (nN)Force (nN)Andrew Hook - P
Page 236 and 237:
Page 238 and 239:
Page 240 and 241:
Page 242 and 243:
show all

Patterned and switchable surfaces for biomaterial applications

You also want an ePaper? Increase the reach of your titles

Delete template?

Save as template?