Patterned and switchable surfaces for biomaterial applications
Patterned and switchable surfaces for biomaterial applications
Patterned and switchable surfaces for biomaterial applications
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Andrew Hook – <strong>Patterned</strong> <strong>and</strong> <strong>switchable</strong> <strong>surfaces</strong> <strong>for</strong> <strong>biomaterial</strong> <strong>applications</strong>4.3. RESULTS AND DISCUSSION ....................................................................................4-1384.3.1. Optimisation of polymer printing .....................................................................4-1384.3.2. Polymer microarray <strong>for</strong>mation ........................................................................4-1394.3.3. Polymer microarray characterisation ..............................................................4-1434.3.4. Cell microarray <strong>for</strong>mation...............................................................................4-1484.4. CONCLUSION .......................................................................................................4-152CHAPTER 5. SURFACE PLASMON RESONANCE IMAGING OF POLYMERMICROARRAYS ................................................................................................ 5-1555.1. INTRODUCTION ....................................................................................................5-1555.2. THEORY BEHIND SURFACE PLASMON RESONANCE IMAGING ....................................5-1585.3. MATERIALS AND METHODS ...................................................................................5-1625.3.1. Substrate preparation......................................................................................5-1625.3.2. Polymerisation of poly(N-isopropylacrylamide) ...............................................5-1635.3.3. Array <strong>for</strong>mation ..............................................................................................5-1635.3.4. SPR imaging ...................................................................................................5-1655.3.5. Study of switching of PNIPAAm by SPR imaging .............................................5-1665.3.6. Cell growth .....................................................................................................5-1675.3.7. Kinetic analysis ...............................................................................................5-1675.4. RESULTS AND DISCUSSION ....................................................................................5-1695.4.1. SPR imaging of a PNIPAAm microarray .........................................................5-1695.4.2. St<strong>and</strong>ardising SPR signal on a polymer microarray .........................................5-1745.4.3. Kinetic analysis of protein adsorption to polymer array ...................................5-1885.4.4. Assessment of cell attachment to polymer arrays .............................................5-2035.5. CONCLUSION .......................................................................................................5-205CHAPTER 6. OVERALL CONCLUSIONS ...................................................... 6-208APPENDIX 1. LIST OF ABBREVIATIONS ...........................................................AAPPENDIX 2. AFM FORCE CURVES OF CROSS-LINKED POLYMER ARRAYS .CA2.1. INTRODUCTION .......................................................................................................... CA2.2. MATERIALS AND METHODS ......................................................................................... DA2.2.1. Plasma polymerisation .......................................................................................... DA2.2.2. Polymer array <strong>for</strong>mation ....................................................................................... DA2.2.3. Polymer spin coating............................................................................................. EA2.2.4. AFM tip modification ............................................................................................ FA2.2.5. AFM <strong>for</strong>ce measurements ...................................................................................... FA2.3. RESULTS .................................................................................................................... GA2.3.1. Polymer spot characterisation ............................................................................... GBIBLIOGRAPHY ................................................................................................ MIII