Patterned and switchable surfaces for biomaterial applications

Chapter 1 - Introduction(A)(B)(C)(C)Figure 1.5. The procedure of dielectrophoretic separation for a monocytic cell andperipheral blood mononuclear cell mixture. (A) The mixture is introduced tothe array. (B) Monocytic cells are separated from peripheral bloodmononuclear cells on array by dielectrophoresis 5 min after an ac voltage of500 kHz frequency and 7 V amplitude is applied. Monocytic cells arecollected on the electrodes and peripheral blood mononuclear cells areaccumulated at the space between the electrodes. (C) Buffer is introducedfrom a reservoir to the array by fluid flow of 40 L/min while the voltage iskept on. Peripheral blood mononuclear cells are carried away with the fluidstream. (D) Peripheral blood mononuclear cells are washed off from thearray and monocytic cells are retained on the electrodes after 10 min ofwashing. From [77].1-28