Patterned and switchable surfaces for biomaterial applications

More documents

Recommendations

Info

Chapter 1 - Introductionby controlling the addition of a particular nucleotide after each activation step, ahigh-density array of tailored sequences can be grown at the surface. This approachhas also been achieved with inkjet technology, enabling probe growth without therigorous protocols required of the photolithography strategy [82, 83]. In situ DNAgrowth enables the formation of an array with millions of probes per cm 2 , however,probes are limited to 25 bases due to low synthesis yields at higher lengths. Thisapproach also requires clean rooms and specialised equipment [82].Laser ablation is another useful approach for micropatterning and can beconsidered a specialised case of photolithography. Here, a high-energy laser beam isdirected on a surface through a patterned mask which leads to ablation of the surfaceunderneath the transparent regions of the photomask. This method suffers from thesame limitations as all photolithographic techniques, but is able to produce highlyresolved patterns of controlled depth at a fast rate, thus, being applicable to theproduction of patterned surface topographies as well as patterned surface chemistries.Thissen, et al. utilised laser ablation with the development of a plasma polymer basedsystem that was shown to spatially confine cell growth [33, 35]. A schematic of theapproach is shown in Figure 1.3. An allylamine plasma polymer (ALAPP) withamine functionality was formed and aldehyde terminated PEG was grafted to thesurface by reductive amination. Subsequent laser ablation produced micronresolution patterned PEG and ALAPP regions (Figure 1.3A and B). Cells wereshown to be confined to the ALAPP region to micron resolution over a four dayperiod using a bovine corneal epithelial cell line (BCEp) (Figure 1.3C) [33]. Thissystem was expanded to include a human embryonic kidney cell (HEK) line [35],and has recently been shown to spatially control protein adsorption [84].1-20
Andrew Hook – Patterned and switchable surfaces for biomaterial applications(A)MASKPEG LAYERPLASMAPOLYMERABLATION(B)(C)200mFigure 1.3. (A) Schematic of two-dimensional patterning of the surface chemistry viaexcimer laser ablation. (B) Light microscopy image of laser ablated Si-ALAPP sample and (C) confocal scanning laser microscopy image of laserablated Si–ALAPP–PEG sample after BCEp cell attachment and stainingwith PicoGreen®. From [33].1-21
Page 1 and 2: Patterned andswitchable surfacesfor
Page 3 and 4: TABLE OF CONTENTSTABLE OF CONTENTS
Page 5 and 6: Andrew Hook - Patterned and switcha
Page 12 and 13: CHAPTER 1.INTRODUCTION1The content
Page 14 and 15: Chapter 1 - Introductionconsiderabl
Page 16 and 17: Chapter 1 - Introductioninteraction
Page 18 and 19: Chapter 1 - IntroductionIn general,
Page 20 and 21: Chapter 1 - IntroductionFor some ap
Page 22 and 23: Chapter 1 - Introductionangles rang
Page 24 and 25: Chapter 1 - Introductionof understa
Page 26 and 27: Chapter 1 - IntroductionSeveral str
Page 28 and 29: Chapter 1 - Introductioncues to con
Page 32 and 33: Chapter 1 - Introduction1.2.2. Soft
Page 34 and 35: Chapter 1 - Introductionsubstrate.
Page 36 and 37: Chapter 1 - Introduction(A)(B)Figur
Page 38 and 39: Chapter 1 - Introduction(A)(B)(C)(C
Page 40 and 41: Chapter 1 - Introductioncomplex gen
Page 42 and 43: Chapter 1 - Introductionmorphology
Page 44 and 45: Chapter 1 - Introduction(A)(B)(C)(D
Page 46 and 47: Chapter 1 - Introductionthe LCST, a
Page 48 and 49: Chapter 1 - IntroductionHyun et al.
Page 50 and 51: Chapter 1 - Introductionmicelles. T
Page 52 and 53: Chapter 1 - Introduction1.4.1. DNA
Page 54 and 55: Chapter 1 - IntroductionA major pro
Page 56 and 57: Chapter 1 - Introductionattached to
Page 58 and 59: Chapter 1 - IntroductionTable 1.1.
Page 60 and 61: Chapter 1 - Introductionefficiencie
Page 62 and 63: Chapter 1 - Introductionachieved by
Page 64 and 65: Chapter 1 - IntroductionTable 1.2.
Page 66 and 67: Chapter 1 - IntroductionOnce releas
Page 68 and 69: Chapter 1 - Introductionimaging not
Page 70 and 71: Chapter 1 - IntroductionA number of
Page 73 and 74: CHAPTER 2.SPATIALLY CONTROLLED ELEC
Page 81 and 82:
Andrew Hook - Patterned and switcha
Page 83 and 84:
Page 85 and 86:
Page 87 and 88:
Page 89 and 90:
Page 91 and 92:
Page 93 and 94:
Page 95 and 96:
Page 97 and 98:
Page 99 and 100:
Page 101 and 102:
Page 103 and 104:
Page 105 and 106:
Page 107 and 108:
CHAPTER 3.COMPARISON OF THE BINDING
Page 109 and 110:
Page 111 and 112:
Page 113 and 114:
Page 115 and 116:
Page 117 and 118:
Page 119 and 120:
Page 121 and 122:
Page 123 and 124:
DNA (mg/m 2 ) DNA (mg/m 2 )Andrew H
Page 125 and 126:
Page 127 and 128:
Page 129 and 130:
Page 131 and 132:
DNA (mg/m 2 )Andrew Hook - Patterne
Page 133 and 134:
Page 135 and 136:
Page 137:
Page 140 and 141:
Chapter 4 - Formation of a chemical
Page 142 and 143:
Page 144 and 145:
Page 146 and 147:
Page 148 and 149:
Page 150 and 151:
Page 152 and 153:
Page 154 and 155:
Page 156 and 157:
Page 158 and 159:
Page 160 and 161:
Page 162 and 163:
Page 165 and 166:
CHAPTER 5.SURFACE PLASMON RESONANCE
Page 167 and 168:
Page 169 and 170:
Page 171 and 172:
Page 173 and 174:
Page 175 and 176:
Page 177 and 178:
Page 179 and 180:
Page 181 and 182:
SPR signal intensity (counts)Andrew
Page 183 and 184:
Page 185 and 186:
Page 187 and 188:
Page 189 and 190:
Page 191 and 192:
Page 193 and 194:
Reflectivity (%) Reflectivity (%)An
Page 195 and 196:
Reflectivity (%)SPR signal intensit
Page 197 and 198:
SPR signal intensity (counts)Andrew
Page 199 and 200:
Page 201 and 202:
Page 203 and 204:
Page 205 and 206:
Thickness (nm)Thickness (nm)Thickne
Page 207 and 208:
Page 209 and 210:
Page 211 and 212:
Page 213 and 214:
Page 215 and 216:
Page 218 and 219:
CHAPTER 6.OVERALL CONCLUSIONS6.6An
Page 220 and 221:
Chapter 6 - Overall conclusionsThes
Page 222 and 223:
Chapter 6 - Overall conclusionsnew
Page 224 and 225:
Page 226 and 227:
Page 228 and 229:
Page 230 and 231:
Page 232 and 233:
Force (nN)Force (nN)Force (nN)Force
Page 234 and 235:
Force (nN)Force (nN)Andrew Hook - P
Page 236 and 237:
Page 238 and 239:
Page 240 and 241:
Page 242 and 243:
show all

Patterned and switchable surfaces for biomaterial applications

You also want an ePaper? Increase the reach of your titles

Delete template?

Save as template?