Patterned and switchable surfaces for biomaterial applications

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Force (nN)Force (nN)Force (nN)Force (nN)Deflection (V)Deflection (V)Andrew Hook – Patterned and switchable surfaces for biomaterial applications(A)50 200 400 600 800(B)50 200 400 600 8004ApproachingReceeding4ApproachingReceeding33221100-10 200 400 600 800 1000 1200-10 200 400 600 800 1000 1200Z-position (nm)Z-position (nm)0 200 400 600 8000 200 400 600 800(C)80(D)8060ApproachingReceeding60ApproachingReceeding4040202000-200 200 400 600 800-200 200 400 600 800Separation (nm)Separation (nm)0 200 400 600 8000 200 400 600 800(E)80(F)8060ApproachingReceeding60ApproachingReceeding40402020000 200 400 600 8000 200 400 600 800Separation (nm)Separation (nm)Figure A1.1. F-D curve measurement for (A, C and E) unmodified and (B, D andF) DNA coated OT 28 tips approaching (A and B) PAA, (C and D) PLL and (Eand F) PVP..J
Andrew Hook – Patterned and switchable surfaces for biomaterial applicationsIn order to characterise the extent of hydrophobic interactions between theAOLG-modified tip and the polymer coatings F-D curves were conducted in TEbuffer supplemented with different % (v/v) of ethanol. The added ethanol decreasesthe free energy of the liquid/solid interface by decreasing the thermodynamic penaltyfor having a polar solvent exposed to hydrophobic moieties on the surface [213].Thus, increasing ethanol content should decrease the driving force for DNAadsorption if hydrophobic interactions play a role [188].A summary of the pull off forces measured between the polymer samples and theDNA is shown as Figure A1.2. Interestingly, no polymer showed a decrease in thepull off force at higher ethanol concentrations, suggesting that hydrophobicinteractions do not play a significant role for the interactions being studied. However,the force between the tip and PALA, PEI and PLL was observed to greatly increaseat higher ethanol concentrations. Ethanol weakly solvates charge compared withwater, thus, at higher ethanol concentrations it is entropically favourable for theformation of polar interactions. Thus, the increase in ethanol concentration enhancedthe electrostatic interactions between the AOLG-modified tip and the polycationicpolymers. This clearly demonstrates the presence of the strong electrostaticinteractions possible between DNA and PALA, PEI and PVP, whilst the ability ofPAA and PVP to repel DNA.K
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Patterned andswitchable surfacesfor
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TABLE OF CONTENTSTABLE OF CONTENTS
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Andrew Hook - Patterned and switcha
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CHAPTER 1.INTRODUCTION1The content
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Chapter 1 - Introductionconsiderabl
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Chapter 1 - Introductioninteraction
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Chapter 1 - IntroductionIn general,
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Chapter 1 - IntroductionFor some ap
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Chapter 1 - Introductionangles rang
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Chapter 1 - Introductionof understa
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Chapter 1 - IntroductionSeveral str
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Chapter 1 - Introductioncues to con
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Chapter 1 - Introductionby controll
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Chapter 1 - Introduction1.2.2. Soft
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Chapter 1 - Introductionsubstrate.
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Chapter 1 - Introduction(A)(B)Figur
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Chapter 1 - Introduction(A)(B)(C)(C
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Chapter 1 - Introductioncomplex gen
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Chapter 1 - Introductionmorphology
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Chapter 1 - Introduction(A)(B)(C)(D
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Chapter 1 - Introductionthe LCST, a
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Chapter 1 - IntroductionHyun et al.
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Chapter 1 - Introductionmicelles. T
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Chapter 1 - Introduction1.4.1. DNA
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Chapter 1 - IntroductionA major pro
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Chapter 1 - Introductionattached to
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Chapter 1 - IntroductionTable 1.1.
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Chapter 1 - Introductionefficiencie
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Chapter 1 - Introductionachieved by
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Chapter 1 - IntroductionTable 1.2.
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Chapter 1 - IntroductionOnce releas
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Chapter 1 - Introductionimaging not
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Chapter 1 - IntroductionA number of
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CHAPTER 2.SPATIALLY CONTROLLED ELEC
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CHAPTER 3.COMPARISON OF THE BINDING
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DNA (mg/m 2 ) DNA (mg/m 2 )Andrew H
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DNA (mg/m 2 )Andrew Hook - Patterne
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Chapter 4 - Formation of a chemical
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CHAPTER 5.SURFACE PLASMON RESONANCE
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Page 181 and 182: SPR signal intensity (counts)Andrew
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Patterned and switchable surfaces for biomaterial applications

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