Patterned and switchable surfaces for biomaterial applications

Andrew Hook – Patterned and switchable surfaces for biomaterial applicationsF-D curves were measured for all polymers and the control samples under fluid inTE buffer. Measurements on the control samples were identical to measurementsdone on individual spots, suggesting the preservation of polymer chemistry whenusing the arraying approach. As expected, a greater pull off force was seen forAOLG-modified tips with polycationic polymers than with unmodified tips or withmodified tips with either PAA or PVP. Representative F-D curves are shown asFigure A1.1. Using the unmodified tip the F-D curve showed no distinctive featurefor any of the polymers (Figure A1.1A, C and E). However, when using the modifiedtip a small repulsive force was observed for the interaction between the tip and PAAas compared with the unmodified tip (Figure A1.1A and B), whilst an attractive forcewas observed for PLL as a pull off force (Figure A1.1D). Similar results were alsoseen with PALA and PEI. The interaction with PVP was unchanged when comparingthe modified and unmodified tip (Figure A1.1E and F).I