Patterned and switchable surfaces for biomaterial applications

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Andrew Hook – Patterned and switchable surfaces for biomaterial applicationsMAAMHAmRNANHSNIH3T3NSANBOCT-1OEGMAPAAPAAcPALAPBSPC12PCRPDMSPEGPEIPEIcpIPLAPLLPLLcPNIPAAmPSPVPIPVPQCMRADSRFPRGDRNARNAiSAMsSK-N-SHSPRSPRissDNATCMTEToF-SIMSUVXPSMethacrylic acid(16-mercapto)hexanoic acidMessenger RNAN-hydroxysuccinimideNormal mouse fibroblastN-succinimidyl-5-azido-2-nitrobenzoateOsteoblast-like cellsOligo(ethylene glycol) methacrylatePoly(acrylic acid)Covalently linked PAA spotPolyallylaminePhosphate buffer salinePheochromocytomaPolymerase chain reactionPoly(dimethylsiloxane)Poly(ethylene glycol)Poly(ethyleneimine)Covalently linked PEI spotIsoelectric pointPoly(lactic acid)Poly(L-lysine)Covalently linked PLL spotPoly(N-isopropylacrylamide)PolystyrenePoly(2-vinyl pyridine)PolyvinylpyrrolidoneQuartz crystal microbalanceArginine-alanine-asparagine-serineRed fluorescing proteinArginine-glycine-asparagineRibonucleic acidRNA interferenceSelf-assembled monolayersNeuroblastomaSurface plasmon resonanceSPR imagingSingle stranded DNATransfected cell microarrayTris-EDTATime of flight secondary ion mass spectroscopyUltravioletX-ray photoelectron spectroscopyB
Andrew Hook – Patterned and switchable surfaces for biomaterial applicationsAPPENDIX 2.AFM FORCE CURVES OF CROSS-LINKEDPOLYMER ARRAYS9.10.A2.1. IntroductionSurface modification has become of increasing importance for a wide range ofapplications. Techniques to modify the outer surface of a material whilst notcompromising the materials bulk properties or integrity are of interest. Of particularinterest are patterned surfaces that are able to offer spatial control over the behaviourof biomolecules at a particular surface. Surface patterning may incorporate bothchemical and topographical features, and can be achieved by a wide range ofapproaches including photolithography, soft lithography, robotic printing,microfluidics and microelectronics [66, 74-77, 79, 133, 197, 214]. A significantoutcome of the development of microfabrication is microarrays. DNA and proteinmicroarrays have revolutionised genomic studies and continue to bring considerableinsight ubiquitously in medical science [98, 127, 135, 309]. Development ofpatterned surface chemistry that precisely match the surface patterns of microarrayscould increase microarray capabilities by allowing higher densities by minimisingthe required separation between adjacent spots, and higher signal to noise ratios byincorporating low-fouling surfaces that minimise non-specific binding. This is ofparticular interest for microarrays containing living cells [1, 45].The formation of a patterned surface, as described in CHAPTER 4, is of particularsuitability for DNA microarray applications. Initially a polycationic polymer havingbioactive properties is functionalised with a phenylazide cross-linker then arrayedonto a low-fouling surface and UV cross-linked to form a covalently linked polymerC
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TABLE OF CONTENTSTABLE OF CONTENTS
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CHAPTER 1.INTRODUCTION1The content
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Chapter 1 - Introductionconsiderabl
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Chapter 1 - Introductioninteraction
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Chapter 1 - IntroductionIn general,
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Chapter 1 - IntroductionFor some ap
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Chapter 1 - Introductionangles rang
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Chapter 1 - Introductionof understa
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Chapter 1 - IntroductionSeveral str
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Chapter 1 - Introductioncues to con
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Chapter 1 - Introductionby controll
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Chapter 1 - Introduction1.2.2. Soft
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Chapter 1 - Introductionsubstrate.
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Chapter 1 - Introduction(A)(B)Figur
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Chapter 1 - Introduction(A)(B)(C)(C
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Chapter 1 - Introductioncomplex gen
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Chapter 1 - Introductionmorphology
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Chapter 1 - Introduction(A)(B)(C)(D
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Chapter 1 - Introductionthe LCST, a
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Chapter 1 - IntroductionHyun et al.
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Chapter 1 - Introduction1.4.1. DNA
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Chapter 1 - IntroductionTable 1.1.
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Chapter 1 - Introductionefficiencie
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Chapter 1 - IntroductionTable 1.2.
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Chapter 1 - IntroductionOnce releas
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Chapter 1 - IntroductionA number of
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CHAPTER 2.SPATIALLY CONTROLLED ELEC
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CHAPTER 3.COMPARISON OF THE BINDING
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DNA (mg/m 2 ) DNA (mg/m 2 )Andrew H
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Chapter 4 - Formation of a chemical
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CHAPTER 5.SURFACE PLASMON RESONANCE
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