Patterned and switchable surfaces for biomaterial applications

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Thickness (nm)Thickness (nm)Thickness (nm)Chapter 5 – Surface plasmon resonance imaging of polymer microarrays(A)0.5(B)0.50.30.30.10.1-0.10 1000 2000-0.10 1000 2000Time (s)Time (s)(C)0.50.30.1-0.10 1000 2000Time (s)Figure 5.16. SPR sensorgrams for the adsorption and desorption of BSA to (A)PAAc, (B) PEIc and (C) PLLc at concentrations of (x) 10 µg/ml, (▲) 5µg/ml, (□) 2 µg/ml and (-) 1 µg/ml at pH 7.4. All curves have had thebackground subtracted (SPR signal on the unmodified HDI PEG coating).Protein was injected once the surface had stabilised in phosphate buffer.Protein injection has been standardised to time=0 for ease of comparison.Buffer was injected as indicated by the arrows.5-196
Andrew Hook – Patterned and switchable surfaces for biomaterial applicationsTable 5.1. Association and dissociation rates, binding constant and total bindingcapacity of CN type I, FN and BSA to PAAc, PEIc and PLLc obtained by fittinga one step kinetic process to kinetic data obtained (Figure 5.14, Figure 5.15and Figure 5.16) by SPRi of a polymer array.k a (s -1 M -1 )(x10 3 )CN type I FN BSAk d (s -1 ) K (M) s∞ k a (s -1 M -1 ) k d (s -1 ) K (M) s∞ k a (s -1 M -1 )(x10 -4 ) (x10 6 ) (mg/m 2 ) (x10 3 ) (x10 -4 ) (x10 6 ) (mg/m 2 ) (x10 3 )k d (s -1 )(x10 -4 )K (M)(x10 6 ) s∞(mg/m 2 )PAA 102.40 0.401 2555.5 15.7 69.94 2.55 274.4 15.3 0.49 26.09 0.2 NAPEI 15.33 2.999 51.1 0.5 35.87 8.67 41.4 3.2 17.38 12.68 13.7 3.8PLL 8.32 3.546 23.5 0.1 88.60 3.55 249.9 0.3 5.81 4.61 12.6 3.2Table 5.2. A biomol (nm 2 ) calculated from the s∞ measured for CN type I, FN and BSAadsorption to PAAc, PEIc and PLLc.CN FN BSAPAA 43 49 NAPEI 1352 234 30PLL 6758 2490 355-197
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Patterned andswitchable surfacesfor
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TABLE OF CONTENTSTABLE OF CONTENTS
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Andrew Hook - Patterned and switcha
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CHAPTER 1.INTRODUCTION1The content
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Chapter 1 - Introductionconsiderabl
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Chapter 1 - Introductioninteraction
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Chapter 1 - IntroductionIn general,
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Chapter 1 - IntroductionFor some ap
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Chapter 1 - Introductionangles rang
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Chapter 1 - Introductionof understa
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Chapter 1 - IntroductionSeveral str
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Chapter 1 - Introductioncues to con
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Chapter 1 - Introductionby controll
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Chapter 1 - Introduction1.2.2. Soft
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Chapter 1 - Introductionsubstrate.
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Chapter 1 - Introduction(A)(B)Figur
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Chapter 1 - Introduction(A)(B)(C)(C
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Chapter 1 - Introductioncomplex gen
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Chapter 1 - Introductionmorphology
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Chapter 1 - Introduction(A)(B)(C)(D
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Chapter 1 - Introductionthe LCST, a
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Chapter 1 - IntroductionHyun et al.
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Chapter 1 - Introductionmicelles. T
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Chapter 1 - Introduction1.4.1. DNA
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Chapter 1 - IntroductionA major pro
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Chapter 1 - Introductionattached to
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Chapter 1 - IntroductionTable 1.1.
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Chapter 1 - Introductionefficiencie
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Chapter 1 - Introductionachieved by
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Chapter 1 - IntroductionTable 1.2.
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Chapter 1 - IntroductionOnce releas
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Chapter 1 - Introductionimaging not
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Chapter 1 - IntroductionA number of
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CHAPTER 2.SPATIALLY CONTROLLED ELEC
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CHAPTER 3.COMPARISON OF THE BINDING
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DNA (mg/m 2 ) DNA (mg/m 2 )Andrew H
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DNA (mg/m 2 )Andrew Hook - Patterne
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Patterned and switchable surfaces for biomaterial applications

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