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Patterned and switchable surfaces for biomaterial applications

Patterned and switchable surfaces for biomaterial applications

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Thickness (nm)Thickness (nm)Thickness (nm)Andrew Hook – <strong>Patterned</strong> <strong>and</strong> <strong>switchable</strong> <strong>surfaces</strong> <strong>for</strong> <strong>biomaterial</strong> <strong>applications</strong>(A)(B)1.01.00.60.60.20.2-0.20 1000 2000 3000Time (s)-0.20 1000 2000 3000Time (s)(C)1.00.60.2-0.20 1000 2000 3000Time (s)Figure 5.15. SPR sensorgrams <strong>for</strong> the adsorption <strong>and</strong> desorption of FN to (A)PAAc, (B) PEIc <strong>and</strong> (C) PLLc at concentrations of (x) 10 µg/ml, (▲) 5µg/ml, (□) 2 µg/ml <strong>and</strong> (-) 1 µg/m at pH 7.4. All curves have had thebackground subtracted (SPR signal on the unmodified HDI PEG coating).Protein was injected once the surface had stabilised in phosphate buffer.Protein injection has been st<strong>and</strong>ardised to time=0 <strong>for</strong> ease of comparison.Buffer was injected as indicated by the arrows.5-195

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