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Patterned and switchable surfaces for biomaterial applications

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Thickness (nm)Thickness (nm)Thickness (nm)Chapter 5 – Surface plasmon resonance imaging of polymer microarrays(A)4.0(B)4.02.52.51.01.0-0.50 2000 4000-0.50 2000 4000Time (s)Time (s)(C)4.02.51.0-0.50 2000 4000Time (s)Figure 5.14. SPR sensorgrams <strong>for</strong> the adsorption <strong>and</strong> desorption of CN type I to(A) PAAc, (B) PEIc <strong>and</strong> (C) PLLc at concentrations of (x) 10 µg/ml, (▲) 5µg/ml, (□) 2 µg/ml <strong>and</strong> (-) 1 µg/ml at pH 7.4. All curves have had thebackground subtracted (SPR signal on the unmodified HDI PEG coating).Protein was injected once the surface had stabilised in phosphate buffer.Protein injection has been st<strong>and</strong>ardised to time=0 <strong>for</strong> ease of comparison.Buffer was injected as indicated by the arrows.5-194

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