Patterned and switchable surfaces for biomaterial applications

Andrew Hook – Patterned and switchable surfaces for biomaterial applicationsAdsorption curves at varied protein concentration were analysed using a one-stepkinetic process, as outlined in section 5.3.7. However, this model is simplistic for aprotein adsorption process, which may include complex processes such as proteinsurfaceinteractions, changes in protein conformation, re-orientation of proteins andsurface diffusion [281]. However, plots of d s /dt against s produced a linear curvewith high R 2 values, as shown for each biomolecular adsorption reaction in 0, withvalues typically >0.97, except when low s was measured such as when 1 g/mlprotein concentrations were used. This suggests that a one-step protein-adsorptionprocess dominates kinetics over the initial time period, thus, for simplicity, thismodel was used to analyse the kinetic data further. The resultant k a , k d , K, and s∞ , asdetermined from equation 5.5 and equation 5.7, for each polymer with each protein isshown in Table 5.1. Assuming monolayer coverage, the area per biomolecule(A biomol ) can be calculated for each s∞ using equation 5.8, where M is the molar massof the protein and N A is Avogadro’s constant = 6.02214x10 23 . The A biomol measuredfor CN, FN and BSA adsorption onto the polymer array are shown in Table 5.2.AbiomolM (5.8)NsA5-193