Patterned and switchable surfaces for biomaterial applications

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Chapter 5 – Surface plasmon resonance imaging of polymer microarraysR 2 values for graphs of d s /dt against s for the adsorption of CN type I, FN and BSAfrom various bulk concentrations to a polymer array of PAAc, PEIc and PLLc.CN type I FN BSA1 2 5 10 1 2 5 10 1 2 5 10g/ml g/ml g/ml g/ml g/ml g/ml g/ml g/ml g/ml g/ml g/ml g/mlPAA 0.526 0.991 0.993 0.991 0.975 0.986 0.944 0.998 0.463 0.954 0.803 0.935PEI 0.831 0.948 0.890 0.959 0.441 0.227 0.974 0.990 0.797 0.987 0.989 0.985PLL 0.787 0.967 0.897 0.978 0.905 0.944 0.993 0.986 0.372 0.994 0.993 0.9945-192
Andrew Hook – Patterned and switchable surfaces for biomaterial applicationsAdsorption curves at varied protein concentration were analysed using a one-stepkinetic process, as outlined in section 5.3.7. However, this model is simplistic for aprotein adsorption process, which may include complex processes such as proteinsurfaceinteractions, changes in protein conformation, re-orientation of proteins andsurface diffusion [281]. However, plots of d s /dt against s produced a linear curvewith high R 2 values, as shown for each biomolecular adsorption reaction in 0, withvalues typically >0.97, except when low s was measured such as when 1 g/mlprotein concentrations were used. This suggests that a one-step protein-adsorptionprocess dominates kinetics over the initial time period, thus, for simplicity, thismodel was used to analyse the kinetic data further. The resultant k a , k d , K, and s∞ , asdetermined from equation 5.5 and equation 5.7, for each polymer with each protein isshown in Table 5.1. Assuming monolayer coverage, the area per biomolecule(A biomol ) can be calculated for each s∞ using equation 5.8, where M is the molar massof the protein and N A is Avogadro’s constant = 6.02214x10 23 . The A biomol measuredfor CN, FN and BSA adsorption onto the polymer array are shown in Table 5.2.AbiomolM (5.8)NsA5-193
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TABLE OF CONTENTSTABLE OF CONTENTS
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CHAPTER 1.INTRODUCTION1The content
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Chapter 1 - Introductionconsiderabl
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Chapter 1 - Introductioninteraction
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Chapter 1 - IntroductionIn general,
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Chapter 1 - IntroductionFor some ap
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Chapter 1 - Introductionangles rang
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Chapter 1 - Introductionof understa
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Chapter 1 - IntroductionSeveral str
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Chapter 1 - Introductioncues to con
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Chapter 1 - Introductionby controll
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Chapter 1 - Introduction1.2.2. Soft
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Chapter 1 - Introductionsubstrate.
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Chapter 1 - Introduction(A)(B)Figur
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Chapter 1 - Introduction(A)(B)(C)(C
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Chapter 1 - Introductioncomplex gen
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Chapter 1 - Introductionmorphology
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Chapter 1 - Introduction(A)(B)(C)(D
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Chapter 1 - Introductionthe LCST, a
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Chapter 1 - IntroductionHyun et al.
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Chapter 1 - Introductionmicelles. T
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Chapter 1 - Introduction1.4.1. DNA
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Chapter 1 - IntroductionA major pro
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Chapter 1 - Introductionattached to
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Chapter 1 - IntroductionTable 1.1.
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Chapter 1 - Introductionefficiencie
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Chapter 1 - Introductionachieved by
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Chapter 1 - IntroductionTable 1.2.
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Chapter 1 - IntroductionOnce releas
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Chapter 1 - Introductionimaging not
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Chapter 1 - IntroductionA number of
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CHAPTER 2.SPATIALLY CONTROLLED ELEC
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CHAPTER 3.COMPARISON OF THE BINDING
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DNA (mg/m 2 ) DNA (mg/m 2 )Andrew H
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Chapter 4 - Formation of a chemical
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