Patterned and switchable surfaces for biomaterial applications

Andrew Hook – Patterned and switchable surfaces for biomaterial applicationsKinetic plots for the adsorption of CN type I, FN and BSA to the polymer arrayare shown in Figure 5.14-Figure 5.16. In order for s∞ to be quantitativelycomparable the measured change in reflectivity must be converted to a shift inresonance angle, as discussed in section 5.4.2. Thus, immediately before and aftersensorgrams were measured, reflectivity versus the angle of incidence was measuredfor each spot. This enabled the measurement of the shift in the resonance angle foreach spot due to the biomolecular interactions and enabled the measured change inreflectivity to be correlated with a shift in the resonance angle. Using the Winspallanalysis software, the shift in resonance angle from each spot could be converted toan increase in biomolecular thickness, assuming a refractive index of this layer of1.45. Assuming a monolayer coverage with a density of 1.35 g/cm 3 [268], thebiomolecular thickness was converted to units of mg/m 2 . This approach enables thestandardisation of the reported SPRi response for an inhomogeneous surface coating.5-191