Patterned and switchable surfaces for biomaterial applications

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Reflectivity (%)Chapter 5 – Surface plasmon resonance imaging of polymer microarrays(A)(B)10080604020050 51 52 53 54Angle of incidence ( o )Figure 5.13. (A) SPR image of representative polymer spots. From top to bottom,spots correspond to PAAc, PEIc, PLLc. Spot diameter is approximately 400m. (B) Reflectivity (%) versus angle of incidence spectra for (■) PAAc, (■)PEIc and (■) PLLc on (■) HDI-PEG coating.5-190
Andrew Hook – Patterned and switchable surfaces for biomaterial applicationsKinetic plots for the adsorption of CN type I, FN and BSA to the polymer arrayare shown in Figure 5.14-Figure 5.16. In order for s∞ to be quantitativelycomparable the measured change in reflectivity must be converted to a shift inresonance angle, as discussed in section 5.4.2. Thus, immediately before and aftersensorgrams were measured, reflectivity versus the angle of incidence was measuredfor each spot. This enabled the measurement of the shift in the resonance angle foreach spot due to the biomolecular interactions and enabled the measured change inreflectivity to be correlated with a shift in the resonance angle. Using the Winspallanalysis software, the shift in resonance angle from each spot could be converted toan increase in biomolecular thickness, assuming a refractive index of this layer of1.45. Assuming a monolayer coverage with a density of 1.35 g/cm 3 [268], thebiomolecular thickness was converted to units of mg/m 2 . This approach enables thestandardisation of the reported SPRi response for an inhomogeneous surface coating.5-191
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Patterned andswitchable surfacesfor
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TABLE OF CONTENTSTABLE OF CONTENTS
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CHAPTER 1.INTRODUCTION1The content
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Chapter 1 - Introductionconsiderabl
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Chapter 1 - Introductioninteraction
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Chapter 1 - IntroductionIn general,
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Chapter 1 - IntroductionFor some ap
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Chapter 1 - Introductionangles rang
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Chapter 1 - Introductionof understa
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Chapter 1 - IntroductionSeveral str
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Chapter 1 - Introductioncues to con
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Chapter 1 - Introductionby controll
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Chapter 1 - Introduction1.2.2. Soft
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Chapter 1 - Introductionsubstrate.
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Chapter 1 - Introduction(A)(B)Figur
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Chapter 1 - Introduction(A)(B)(C)(C
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Chapter 1 - Introductioncomplex gen
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Chapter 1 - Introductionmorphology
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Chapter 1 - Introduction(A)(B)(C)(D
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Chapter 1 - Introductionthe LCST, a
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Chapter 1 - IntroductionHyun et al.
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Chapter 1 - Introductionmicelles. T
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Chapter 1 - Introduction1.4.1. DNA
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Chapter 1 - IntroductionA major pro
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Chapter 1 - Introductionattached to
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Chapter 1 - IntroductionTable 1.1.
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Chapter 1 - Introductionefficiencie
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Chapter 1 - Introductionachieved by
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Chapter 1 - IntroductionTable 1.2.
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Chapter 1 - IntroductionOnce releas
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Chapter 1 - Introductionimaging not
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Chapter 1 - IntroductionA number of
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CHAPTER 2.SPATIALLY CONTROLLED ELEC
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CHAPTER 3.COMPARISON OF THE BINDING
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DNA (mg/m 2 ) DNA (mg/m 2 )Andrew H
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DNA (mg/m 2 )Andrew Hook - Patterne
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Chapter 4 - Formation of a chemical
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Patterned and switchable surfaces for biomaterial applications

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