Patterned and switchable surfaces for biomaterial applications

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Chapter 5 – Surface plasmon resonance imaging of polymer microarraysThis approach was conducted for a larger polymer array consisting of PAA, PEI,and PLL (for structures see Figure 4.3B-D) spotted at concentrations of 2.0, 1.0, 0.5,0.25, 0.1, 0.05 and 0.01 mg/ml, resulting in an array of 21 polymer spots of variedthickness and refractive index. Similar to the experiment described above, this arraywas first equilibrated in phosphate buffer and the reflectivity for a range of angles ofincidence was measured for each spot. Then a fixed angle experiment at 48.9° wasconducted to monitor the change in SPR signal intensity versus time for the exchangeof phosphate buffer with 1% ethanol. The resultant sensorgram is shown as Figure5.12A. Subsequently, the measurement of reflectivity for a range of angles ofincidence was repeated after ethanol injection and the measured change in theresonance angle was used to convert the change in SPR signal intensity measured forthe fixed angle experiment (Figure 5.12A) to a change in resonance angle againsttime, shown as Figure 5.12B. Significantly, the resultant shift in resonance anglemeasured for the majority of spots converged to -0.141° as expected. Only four spotsdiffered significantly from the expected value, which had thicknesses greater than 15nm.5-186
SPR signal intensity (counts)Andrew Hook – Patterned and switchable surfaces for biomaterial applications(A)50Ethanol injectedEthanol injectedEthanol injected-5-10-15-20-25-300 1000 2000 3000Time (s)(B)0.040Ethanol injectedresonance angle ( o )-0.04-0.08-0.12-0.160 1000 2000 3000Time (s)Figure 5.12. SPRi measurements of a polymer array including PAA, PEI and PLL;(A) sensorgram for each spot. 1% ethanol solution was added at 1000 s, (B)resultant resonance angle versus time curve for the addition of 1% ethanolsolution after converting the measured change in SPR signal intensity from(A) into a shift in the resonance angle.5-187
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Patterned andswitchable surfacesfor
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TABLE OF CONTENTSTABLE OF CONTENTS
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Andrew Hook - Patterned and switcha
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CHAPTER 1.INTRODUCTION1The content
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Chapter 1 - Introductionconsiderabl
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Chapter 1 - Introductioninteraction
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Chapter 1 - IntroductionIn general,
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Chapter 1 - IntroductionFor some ap
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Chapter 1 - Introductionangles rang
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Chapter 1 - Introductionof understa
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Chapter 1 - IntroductionSeveral str
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Chapter 1 - Introductioncues to con
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Chapter 1 - Introductionby controll
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Chapter 1 - Introduction1.2.2. Soft
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Chapter 1 - Introductionsubstrate.
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Chapter 1 - Introduction(A)(B)Figur
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Chapter 1 - Introduction(A)(B)(C)(C
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Chapter 1 - Introductioncomplex gen
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Chapter 1 - Introductionmorphology
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Chapter 1 - Introduction(A)(B)(C)(D
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Chapter 1 - Introductionthe LCST, a
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Chapter 1 - IntroductionHyun et al.
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Chapter 1 - Introductionmicelles. T
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Chapter 1 - Introduction1.4.1. DNA
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Chapter 1 - IntroductionA major pro
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Chapter 1 - Introductionattached to
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Chapter 1 - IntroductionTable 1.1.
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Chapter 1 - Introductionefficiencie
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Chapter 1 - Introductionachieved by
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Chapter 1 - IntroductionTable 1.2.
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Chapter 1 - IntroductionOnce releas
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Chapter 1 - Introductionimaging not
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Chapter 1 - IntroductionA number of
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CHAPTER 2.SPATIALLY CONTROLLED ELEC
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CHAPTER 3.COMPARISON OF THE BINDING
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DNA (mg/m 2 ) DNA (mg/m 2 )Andrew H
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DNA (mg/m 2 )Andrew Hook - Patterne
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Chapter 4 - Formation of a chemical
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