Patterned and switchable surfaces for biomaterial applications

Andrew Hook – Patterned and switchable surfaces for biomaterial applicationsFigure 5.8. Schematic depicting the method used to normalise a measured change inreflectivity to the shift in the resonance angle. On a given SPR chip surfacean adsorption event would result in a shift in the reflectivity versus angle ofincidence curve from the solid to the dotted curve, resulting in a shift in theresonance curve corresponding to the arrow labelled A. However, for afixed angle SPR measurement, at an angle of incidence β, the change inreflectivity, corresponding to the arrow labelled B, is measured. Using theinitial (solid) reflectivity versus angle of incidence curve the correspondingshift in resonance angle C can be determined, whereupon C is found to beequal to A. Using this approach every change in reflectivity measuredduring a fixed angle experiment can be converted to the corresponding shiftin resonance angle.5-181